In vitro ACTIVITY OF THE ANTIBIOTIC BATUMIN AGAINST Candida albicans BIOFILM
The aim of this work was to study action of batumin on the strains of Candida albicans and Candida krusei in planktonic and biofilm form and also to obtain more detailed insights into the influence of batumin on biofilm formation by using atomic-force microscopy. The Minimum Inhibitory Concentrat...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2020-06-01
|
| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/3_2020/churkina_3_2020.pdf |
| _version_ | 1797709169283825664 |
|---|---|
| author | L. N. Churkina N. B. Perunova О. V. Bukharin Е. V. Ivanova L. V. Yaroshenko |
| author_facet | L. N. Churkina N. B. Perunova О. V. Bukharin Е. V. Ivanova L. V. Yaroshenko |
| author_sort | L. N. Churkina |
| collection | DOAJ |
| description | The aim of this work was to study action of batumin on the strains of Candida albicans and
Candida krusei in planktonic and biofilm form and also to obtain more detailed insights into the
influence of batumin on biofilm formation by using atomic-force microscopy. The Minimum
Inhibitory Concentration (MIC) of batumin was studied according to CLSI standards. Formation of
a biofilm was studied by the photometric O’Toole method by means of a plate photometer ELx808
(BioTek, USA) at wavelength of 630 nanometers.
The batumin has a high selective activity against staphylococci (MIC 0,25 μg/ml), at the same
time, antibiotic, being not active concerning yeast of the genus Candida (MIC 512 μg/ml) showed
the inhibiting action on biofilm formation of these microorganisms. Batumin influence on biofilm
formation was studied in type, collection strains С. albicans, C. krusei and clinical isolates.
Presence 0,125 μg/ml of batumin in the broth (1/2 MIC for staphylococci) reduced the biofilm
formation at 55.6% of the studied strains. Their biofilm formation values varied for C. albicans
from 1.5–3.9 CU (conventional unit: OD630 in experimental samples/OD630 in control samples), for
C. krusei of 2.3–3.0. Batumin was more effective against Candida strains with strong biofilm
formation.
Atomic force microscopy revealed qualitative changes in the exopolymeric matrix due to
batumin treatment, as well as a significant reduction in the number of cells adhered to the
coverslip, preventing formation of C. albicans 127 biofilm, However, C. albicans ATCC 24433 a
significant reduction in the number of cells adhered to the coverslip weren’t observed.
The data obtained by an Аtomic force microscopy confirm ability of a batumin to prevent
formation of a biofilm at the studied strains that allows to consider it as the preventive agent at
treatment of the infections caused by yeast-like fungi of the genus Candida. |
| first_indexed | 2024-03-12T06:32:29Z |
| format | Article |
| id | doaj.art-da2a2d8a3675407cab26fc3574974151 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T06:32:29Z |
| publishDate | 2020-06-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-da2a2d8a3675407cab26fc35749741512023-09-03T01:34:03ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2020-06-01133738010.15407/biotech13.03.073In vitro ACTIVITY OF THE ANTIBIOTIC BATUMIN AGAINST Candida albicans BIOFILML. N. Churkina 0 N. B. Perunova 1 О. V. Bukharin 2Е. V. Ivanova 3L. V. Yaroshenko 4Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, KyivInstitute of Cellular and Intracellular Symbiosis, Ural Branch of Russian Academy of Sciences, Orenburg, Russian FederationInstitute of Cellular and Intracellular Symbiosis, Ural Branch of Russian Academy of Sciences, Orenburg, Russian FederationInstitute of Cellular and Intracellular Symbiosis, Ural Branch of Russian Academy of Sciences, Orenburg, Russian FederationZabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, KyivThe aim of this work was to study action of batumin on the strains of Candida albicans and Candida krusei in planktonic and biofilm form and also to obtain more detailed insights into the influence of batumin on biofilm formation by using atomic-force microscopy. The Minimum Inhibitory Concentration (MIC) of batumin was studied according to CLSI standards. Formation of a biofilm was studied by the photometric O’Toole method by means of a plate photometer ELx808 (BioTek, USA) at wavelength of 630 nanometers. The batumin has a high selective activity against staphylococci (MIC 0,25 μg/ml), at the same time, antibiotic, being not active concerning yeast of the genus Candida (MIC 512 μg/ml) showed the inhibiting action on biofilm formation of these microorganisms. Batumin influence on biofilm formation was studied in type, collection strains С. albicans, C. krusei and clinical isolates. Presence 0,125 μg/ml of batumin in the broth (1/2 MIC for staphylococci) reduced the biofilm formation at 55.6% of the studied strains. Their biofilm formation values varied for C. albicans from 1.5–3.9 CU (conventional unit: OD630 in experimental samples/OD630 in control samples), for C. krusei of 2.3–3.0. Batumin was more effective against Candida strains with strong biofilm formation. Atomic force microscopy revealed qualitative changes in the exopolymeric matrix due to batumin treatment, as well as a significant reduction in the number of cells adhered to the coverslip, preventing formation of C. albicans 127 biofilm, However, C. albicans ATCC 24433 a significant reduction in the number of cells adhered to the coverslip weren’t observed. The data obtained by an Аtomic force microscopy confirm ability of a batumin to prevent formation of a biofilm at the studied strains that allows to consider it as the preventive agent at treatment of the infections caused by yeast-like fungi of the genus Candida.http://biotechnology.kiev.ua/images/storage/3_2020/churkina_3_2020.pdfbatumincandidabiofilmatomic force microscopy. |
| spellingShingle | L. N. Churkina N. B. Perunova О. V. Bukharin Е. V. Ivanova L. V. Yaroshenko In vitro ACTIVITY OF THE ANTIBIOTIC BATUMIN AGAINST Candida albicans BIOFILM Biotechnologia Acta batumin candida biofilm atomic force microscopy. |
| title | In vitro ACTIVITY OF THE ANTIBIOTIC BATUMIN AGAINST Candida albicans BIOFILM |
| title_full | In vitro ACTIVITY OF THE ANTIBIOTIC BATUMIN AGAINST Candida albicans BIOFILM |
| title_fullStr | In vitro ACTIVITY OF THE ANTIBIOTIC BATUMIN AGAINST Candida albicans BIOFILM |
| title_full_unstemmed | In vitro ACTIVITY OF THE ANTIBIOTIC BATUMIN AGAINST Candida albicans BIOFILM |
| title_short | In vitro ACTIVITY OF THE ANTIBIOTIC BATUMIN AGAINST Candida albicans BIOFILM |
| title_sort | in vitro activity of the antibiotic batumin against candida albicans biofilm |
| topic | batumin candida biofilm atomic force microscopy. |
| url | http://biotechnology.kiev.ua/images/storage/3_2020/churkina_3_2020.pdf |
| work_keys_str_mv | AT lnchurkina invitroactivityoftheantibioticbatuminagainstcandidaalbicansbiofilm AT nbperunova invitroactivityoftheantibioticbatuminagainstcandidaalbicansbiofilm AT ovbukharin invitroactivityoftheantibioticbatuminagainstcandidaalbicansbiofilm AT evivanova invitroactivityoftheantibioticbatuminagainstcandidaalbicansbiofilm AT lvyaroshenko invitroactivityoftheantibioticbatuminagainstcandidaalbicansbiofilm |