Skin proteomic profiling of irradiation-induced fibrosis and its modulation by low molecular weight fucoidan via tight junction pathway
Fucoidans, sulfated and fucosylated polysaccharides extracted from brown seaweed, were found to inhibit radiotherapy-induced cell damage and fibrosis through the TGF-β1 pathway. However, the comprehensive molecular response during irradiation-induced fibrosis and fucoidan-assisted recovery still rem...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Biomedicine & Pharmacotherapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S075333222200806X |
| _version_ | 1811188117145649152 |
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| author | Pang-Hung Hsu Yi-Hsuan Chen Pin-I. Huang Pai-An Hwang |
| author_facet | Pang-Hung Hsu Yi-Hsuan Chen Pin-I. Huang Pai-An Hwang |
| author_sort | Pang-Hung Hsu |
| collection | DOAJ |
| description | Fucoidans, sulfated and fucosylated polysaccharides extracted from brown seaweed, were found to inhibit radiotherapy-induced cell damage and fibrosis through the TGF-β1 pathway. However, the comprehensive molecular response during irradiation-induced fibrosis and fucoidan-assisted recovery still remain unclear. Rat hind limbs were irradiated and smeared with low molecular weight fucoidan (LMF). Protein profiles were examined by a mass spectrometry-based proteomics analysis. Out of a total of 4625 proteins, 233 were found to be significantly up-regulated after irradiation and down-regulated after LMF treatment. Pathway and protein-protein interaction network analyses further indicated that four proteins including Actb, Ezr, Msn and Cdc42 were clustered into the tight junction and regulation of actin cytoskeleton pathways. These four proteins may serve as biomarkers for the detection of skin fibrosis induced by irradiation or TGF-β1, and for the recovery following LMF treatment. |
| first_indexed | 2024-04-11T14:14:06Z |
| format | Article |
| id | doaj.art-da2bb24ea0504825ae9c20ef76a32c71 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-11T14:14:06Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-da2bb24ea0504825ae9c20ef76a32c712022-12-22T04:19:36ZengElsevierBiomedicine & Pharmacotherapy0753-33222022-09-01153113417Skin proteomic profiling of irradiation-induced fibrosis and its modulation by low molecular weight fucoidan via tight junction pathwayPang-Hung Hsu0Yi-Hsuan Chen1Pin-I. Huang2Pai-An Hwang3Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan; Bachelor Degree Program in Marine Biotechnology, National Taiwan Ocean University, Keelung, Taiwan; Institute of Biochemistry and Molecular Biology, National Yang Ming Chiao Tung University, Taipei, Taiwan; Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, TaiwanDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, TaiwanDepartment of Oncology, Division of Radiation Oncology, Taipei Veterans General Hospital School of Medicine, National Yang Ming Chiao Tung University, Taipei, TaiwanDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan; Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan; Corresponding author at: Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan.Fucoidans, sulfated and fucosylated polysaccharides extracted from brown seaweed, were found to inhibit radiotherapy-induced cell damage and fibrosis through the TGF-β1 pathway. However, the comprehensive molecular response during irradiation-induced fibrosis and fucoidan-assisted recovery still remain unclear. Rat hind limbs were irradiated and smeared with low molecular weight fucoidan (LMF). Protein profiles were examined by a mass spectrometry-based proteomics analysis. Out of a total of 4625 proteins, 233 were found to be significantly up-regulated after irradiation and down-regulated after LMF treatment. Pathway and protein-protein interaction network analyses further indicated that four proteins including Actb, Ezr, Msn and Cdc42 were clustered into the tight junction and regulation of actin cytoskeleton pathways. These four proteins may serve as biomarkers for the detection of skin fibrosis induced by irradiation or TGF-β1, and for the recovery following LMF treatment.http://www.sciencedirect.com/science/article/pii/S075333222200806XFucoidanRadiotherapyFibrosisProteomicsProtein interaction mapsTight junctions |
| spellingShingle | Pang-Hung Hsu Yi-Hsuan Chen Pin-I. Huang Pai-An Hwang Skin proteomic profiling of irradiation-induced fibrosis and its modulation by low molecular weight fucoidan via tight junction pathway Biomedicine & Pharmacotherapy Fucoidan Radiotherapy Fibrosis Proteomics Protein interaction maps Tight junctions |
| title | Skin proteomic profiling of irradiation-induced fibrosis and its modulation by low molecular weight fucoidan via tight junction pathway |
| title_full | Skin proteomic profiling of irradiation-induced fibrosis and its modulation by low molecular weight fucoidan via tight junction pathway |
| title_fullStr | Skin proteomic profiling of irradiation-induced fibrosis and its modulation by low molecular weight fucoidan via tight junction pathway |
| title_full_unstemmed | Skin proteomic profiling of irradiation-induced fibrosis and its modulation by low molecular weight fucoidan via tight junction pathway |
| title_short | Skin proteomic profiling of irradiation-induced fibrosis and its modulation by low molecular weight fucoidan via tight junction pathway |
| title_sort | skin proteomic profiling of irradiation induced fibrosis and its modulation by low molecular weight fucoidan via tight junction pathway |
| topic | Fucoidan Radiotherapy Fibrosis Proteomics Protein interaction maps Tight junctions |
| url | http://www.sciencedirect.com/science/article/pii/S075333222200806X |
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