OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice
Iron (Fe) homeostasis is essential for plant growth and development, and it is strictly regulated by a group of transcriptional factors. Iron-related transcription factor 3 (OsIRO3) was previously identified as a negative regulator for Fe deficiency response in rice. However, the molecular mechanism...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/9/1095 |
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| author | Wujian Wang Jun Ye Yanran Ma Ting Wang Huixia Shou Luqing Zheng |
| author_facet | Wujian Wang Jun Ye Yanran Ma Ting Wang Huixia Shou Luqing Zheng |
| author_sort | Wujian Wang |
| collection | DOAJ |
| description | Iron (Fe) homeostasis is essential for plant growth and development, and it is strictly regulated by a group of transcriptional factors. Iron-related transcription factor 3 (OsIRO3) was previously identified as a negative regulator for Fe deficiency response in rice. However, the molecular mechanisms by which OsIRO3 regulate Fe homeostasis is unclear. Here, we report that OsIRO3 is essential for responding to Fe deficiency and maintaining Fe homeostasis in rice. OsIRO3 is expressed in the roots, leaves, and base nodes, with a higher level in leaf blades at the vegetative growth stage. Knockout of <i>OsIRO3</i> resulted in a hypersensitivity to Fe deficiency, with severe necrosis on young leaves and defective root development. The <i>iro3</i> mutants accumulated higher levels of Fe in the shoot under Fe-deficient conditions, associated with upregulating the expression of <i>OsNAS3</i>, which lead to increased accumulation of nicotianamine (NA) in the roots. Further analysis indicated that OsIRO3 can directly bind to the E-box in the promoter of <i>OsNAS3</i>. Moreover, the expression of typical Fe-related genes was significantly up-regulated in <i>iro3</i> mutants under Fe-sufficient conditions. Thus, we conclude that OsIRO3 plays a key role in responding to Fe deficiency and regulates NA levels by directly, negatively regulating the <i>OsNAS3</i> expression. |
| first_indexed | 2024-03-10T16:50:13Z |
| format | Article |
| id | doaj.art-da2cbe61a0f44c0bb019f3fe809a93e2 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T16:50:13Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-da2cbe61a0f44c0bb019f3fe809a93e22023-11-20T11:20:56ZengMDPI AGPlants2223-77472020-08-0199109510.3390/plants9091095OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in RiceWujian Wang0Jun Ye1Yanran Ma2Ting Wang3Huixia Shou4Luqing Zheng5College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaIron (Fe) homeostasis is essential for plant growth and development, and it is strictly regulated by a group of transcriptional factors. Iron-related transcription factor 3 (OsIRO3) was previously identified as a negative regulator for Fe deficiency response in rice. However, the molecular mechanisms by which OsIRO3 regulate Fe homeostasis is unclear. Here, we report that OsIRO3 is essential for responding to Fe deficiency and maintaining Fe homeostasis in rice. OsIRO3 is expressed in the roots, leaves, and base nodes, with a higher level in leaf blades at the vegetative growth stage. Knockout of <i>OsIRO3</i> resulted in a hypersensitivity to Fe deficiency, with severe necrosis on young leaves and defective root development. The <i>iro3</i> mutants accumulated higher levels of Fe in the shoot under Fe-deficient conditions, associated with upregulating the expression of <i>OsNAS3</i>, which lead to increased accumulation of nicotianamine (NA) in the roots. Further analysis indicated that OsIRO3 can directly bind to the E-box in the promoter of <i>OsNAS3</i>. Moreover, the expression of typical Fe-related genes was significantly up-regulated in <i>iro3</i> mutants under Fe-sufficient conditions. Thus, we conclude that OsIRO3 plays a key role in responding to Fe deficiency and regulates NA levels by directly, negatively regulating the <i>OsNAS3</i> expression.https://www.mdpi.com/2223-7747/9/9/1095riceironFe deficiencytranscription factorOsIRO3OsNAS3 |
| spellingShingle | Wujian Wang Jun Ye Yanran Ma Ting Wang Huixia Shou Luqing Zheng OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice Plants rice iron Fe deficiency transcription factor OsIRO3 OsNAS3 |
| title | OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice |
| title_full | OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice |
| title_fullStr | OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice |
| title_full_unstemmed | OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice |
| title_short | OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice |
| title_sort | osiro3 plays an essential role in iron deficiency responses and regulates iron homeostasis in rice |
| topic | rice iron Fe deficiency transcription factor OsIRO3 OsNAS3 |
| url | https://www.mdpi.com/2223-7747/9/9/1095 |
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