Association of advanced glycation end products in Dupuytren disease

Abstract Background Advanced glycation end products are associated with aging, hyperglycemia, and oxidative stress. Accumulation of advanced glycation end products can cause various pathological conditions; however, the association of Dupuytren’s disease with advanced glycation end products has not...

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Main Authors: Fumiaki Takase, Yutaka Mifune, Atsuyuki Inui, Yasuhiro Ueda, Takeshi Kataoka, Takeshi Kokubu, Ryosuke Kuroda
Format: Article
Language:English
Published: BMC 2018-06-01
Series:Journal of Orthopaedic Surgery and Research
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13018-018-0848-4
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author Fumiaki Takase
Yutaka Mifune
Atsuyuki Inui
Yasuhiro Ueda
Takeshi Kataoka
Takeshi Kokubu
Ryosuke Kuroda
author_facet Fumiaki Takase
Yutaka Mifune
Atsuyuki Inui
Yasuhiro Ueda
Takeshi Kataoka
Takeshi Kokubu
Ryosuke Kuroda
author_sort Fumiaki Takase
collection DOAJ
description Abstract Background Advanced glycation end products are associated with aging, hyperglycemia, and oxidative stress. Accumulation of advanced glycation end products can cause various pathological conditions; however, the association of Dupuytren’s disease with advanced glycation end products has not been demonstrated yet. The aim of this study is to investigate the association of Dupuytren’s disease with advanced glycation end products. Methods Normal palmar fascia from five patients with carpal tunnel syndrome (control group) and Dupuytren’s cords from five patients (Dupuytren’s disease group) were harvested. The tissues were stained using an anti-advanced glycation end products antibody, anti-receptor for advanced glycation end products antibody, and an anti-reactive oxygen species modulator 1 antibody. The expression of nicotinamide adenine dinucleotide phosphate oxidase-1 and nicotinamide adenine dinucleotide phosphate oxidase-4 genes was also assessed using real-time PCR. For in vitro analysis, the cells harvested from the control and Dupuytren’s disease groups were used. After 3 days of exposure to four types of media (control group, control + advanced glycation end products group, Dupuytren’s disease group, Dupuytren’s disease + advanced glycation end products group), superoxide detection reagent was detected using a total reactive oxygen species/superoxide detection kit. Results Immunostaining of the palmar fasciae of the Dupuytren’s disease group showed higher expressions of advanced glycation end products and receptor for advanced glycation end products than that in the control group. The expression of nicotinamide adenine dinucleotide phosphate oxidase oxidase-1 and nicotinamide adenine dinucleotide phosphate oxidase-4 as well as reactive oxygen species modulator 1, an oxidatively damaged protein, was also higher in the Dupuytren’s disease group than in the control group. In an in vitro cell culture, the addition of advanced glycation end products to the Dupuytren’s disease-derived cells produced more superoxide free radicals. Conclusions These data suggest that the advanced glycation end products receptor for advanced glycation end products interaction produced free radicals via nicotinamide adenine dinucleotide phosphate oxidase activation in Dupuytren’s disease patients. Further studies are required to confirm these results.
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spelling doaj.art-da2cc36ec1634896bc785d34053729872022-12-22T01:57:53ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2018-06-011311710.1186/s13018-018-0848-4Association of advanced glycation end products in Dupuytren diseaseFumiaki Takase0Yutaka Mifune1Atsuyuki Inui2Yasuhiro Ueda3Takeshi Kataoka4Takeshi Kokubu5Ryosuke Kuroda6Department of Orthopedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopedic Surgery, Kobe University Graduate School of MedicineDepartment of Orthopedic Surgery, Kobe University Graduate School of MedicineAbstract Background Advanced glycation end products are associated with aging, hyperglycemia, and oxidative stress. Accumulation of advanced glycation end products can cause various pathological conditions; however, the association of Dupuytren’s disease with advanced glycation end products has not been demonstrated yet. The aim of this study is to investigate the association of Dupuytren’s disease with advanced glycation end products. Methods Normal palmar fascia from five patients with carpal tunnel syndrome (control group) and Dupuytren’s cords from five patients (Dupuytren’s disease group) were harvested. The tissues were stained using an anti-advanced glycation end products antibody, anti-receptor for advanced glycation end products antibody, and an anti-reactive oxygen species modulator 1 antibody. The expression of nicotinamide adenine dinucleotide phosphate oxidase-1 and nicotinamide adenine dinucleotide phosphate oxidase-4 genes was also assessed using real-time PCR. For in vitro analysis, the cells harvested from the control and Dupuytren’s disease groups were used. After 3 days of exposure to four types of media (control group, control + advanced glycation end products group, Dupuytren’s disease group, Dupuytren’s disease + advanced glycation end products group), superoxide detection reagent was detected using a total reactive oxygen species/superoxide detection kit. Results Immunostaining of the palmar fasciae of the Dupuytren’s disease group showed higher expressions of advanced glycation end products and receptor for advanced glycation end products than that in the control group. The expression of nicotinamide adenine dinucleotide phosphate oxidase oxidase-1 and nicotinamide adenine dinucleotide phosphate oxidase-4 as well as reactive oxygen species modulator 1, an oxidatively damaged protein, was also higher in the Dupuytren’s disease group than in the control group. In an in vitro cell culture, the addition of advanced glycation end products to the Dupuytren’s disease-derived cells produced more superoxide free radicals. Conclusions These data suggest that the advanced glycation end products receptor for advanced glycation end products interaction produced free radicals via nicotinamide adenine dinucleotide phosphate oxidase activation in Dupuytren’s disease patients. Further studies are required to confirm these results.http://link.springer.com/article/10.1186/s13018-018-0848-4Dupuytren’s diseaseAdvanced glycation end products (AGEs)Oxidation
spellingShingle Fumiaki Takase
Yutaka Mifune
Atsuyuki Inui
Yasuhiro Ueda
Takeshi Kataoka
Takeshi Kokubu
Ryosuke Kuroda
Association of advanced glycation end products in Dupuytren disease
Journal of Orthopaedic Surgery and Research
Dupuytren’s disease
Advanced glycation end products (AGEs)
Oxidation
title Association of advanced glycation end products in Dupuytren disease
title_full Association of advanced glycation end products in Dupuytren disease
title_fullStr Association of advanced glycation end products in Dupuytren disease
title_full_unstemmed Association of advanced glycation end products in Dupuytren disease
title_short Association of advanced glycation end products in Dupuytren disease
title_sort association of advanced glycation end products in dupuytren disease
topic Dupuytren’s disease
Advanced glycation end products (AGEs)
Oxidation
url http://link.springer.com/article/10.1186/s13018-018-0848-4
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