Validation of a Novel Potentiometric Method Based on a Polymeric PVC Membrane Sensor Integrated with Tailored Receptors for the Antileukemia Drug Cytarabine
A simple, rapid and easy method is proposed for the detection of a cytostatic therapeutic drug, cytarabine, in real samples. The method is based on potentiometric transduction using prepared and characterized new ion-selective electrodes for cytarabine. The electrodes were integrated with novel man-...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/6/1343 |
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| author | Ayman H. Kamel Abd El-Galil E. Amr Nashwa H. Ashmawy Hoda R. Galal Abdulrahman A. Almehizia Teraze A. Youssef Mohamed A. Al-Omar Ahmed Y. A. Sayed |
| author_facet | Ayman H. Kamel Abd El-Galil E. Amr Nashwa H. Ashmawy Hoda R. Galal Abdulrahman A. Almehizia Teraze A. Youssef Mohamed A. Al-Omar Ahmed Y. A. Sayed |
| author_sort | Ayman H. Kamel |
| collection | DOAJ |
| description | A simple, rapid and easy method is proposed for the detection of a cytostatic therapeutic drug, cytarabine, in real samples. The method is based on potentiometric transduction using prepared and characterized new ion-selective electrodes for cytarabine. The electrodes were integrated with novel man-tailored imprinted polymers and used as a sensory element for recognition. The electrodes revealed a remarkable potentiometric response for cytarabine over the linearity range 1.0 × 10<sup>−6</sup>–1.0 × 10<sup>−3</sup> M at pH 2.8–4 with a detection limit of 5.5 × 10<sup>−7</sup> M. The potentiometric response was near-Nernstian, with average slopes of 52.3 ± 1.2 mV/decade. The effect of lipophilic salts and plasticizer types on the potentiometric response was also examined. The electrodes exhibited an enhanced selectivity towards cytarabine over various foreign common ions. Validation and verification of the presented assay method are demonstrated by evaluating the method ruggedness and calculating the detection limit, range of linearity, accuracy (trueness), precision, repeatability (within-day) and reproducibility (between-days). The proposed ion-selective electrodes revealed good performance characteristics and possible application of these electrodes for cytarabine monitoring in different matrices. The electrodes are successfully applied to cytarabine determination in spiked biological fluid samples and in pharmaceutical formulations. |
| first_indexed | 2024-03-10T19:11:32Z |
| format | Article |
| id | doaj.art-da2e6594d5a34beda5073b50033c98f3 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T19:11:32Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-da2e6594d5a34beda5073b50033c98f32023-11-20T03:46:14ZengMDPI AGPolymers2073-43602020-06-01126134310.3390/polym12061343Validation of a Novel Potentiometric Method Based on a Polymeric PVC Membrane Sensor Integrated with Tailored Receptors for the Antileukemia Drug CytarabineAyman H. Kamel0Abd El-Galil E. Amr1Nashwa H. Ashmawy2Hoda R. Galal3Abdulrahman A. Almehizia4Teraze A. Youssef5Mohamed A. Al-Omar6Ahmed Y. A. Sayed7Chemistry Department, Faculty of Science, Ain Shams University, P.O. Cairo 11566, EgyptPharmaceutical Chemistry Department, Drug Exploration & Development Chair (DEDC), College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaChemistry Department, Faculty of Science, Ain Shams University, P.O. Cairo 11566, EgyptInorganic Chemistry Department, National Research Center, Dokki, Giza 12622, EgyptPharmaceutical Chemistry Department, Drug Exploration & Development Chair (DEDC), College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaChemistry Department, Faculty of Science, Ain Shams University, P.O. Cairo 11566, EgyptPharmaceutical Chemistry Department, Drug Exploration & Development Chair (DEDC), College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaPharmaceutical Chemistry Department, Drug Exploration & Development Chair (DEDC), College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaA simple, rapid and easy method is proposed for the detection of a cytostatic therapeutic drug, cytarabine, in real samples. The method is based on potentiometric transduction using prepared and characterized new ion-selective electrodes for cytarabine. The electrodes were integrated with novel man-tailored imprinted polymers and used as a sensory element for recognition. The electrodes revealed a remarkable potentiometric response for cytarabine over the linearity range 1.0 × 10<sup>−6</sup>–1.0 × 10<sup>−3</sup> M at pH 2.8–4 with a detection limit of 5.5 × 10<sup>−7</sup> M. The potentiometric response was near-Nernstian, with average slopes of 52.3 ± 1.2 mV/decade. The effect of lipophilic salts and plasticizer types on the potentiometric response was also examined. The electrodes exhibited an enhanced selectivity towards cytarabine over various foreign common ions. Validation and verification of the presented assay method are demonstrated by evaluating the method ruggedness and calculating the detection limit, range of linearity, accuracy (trueness), precision, repeatability (within-day) and reproducibility (between-days). The proposed ion-selective electrodes revealed good performance characteristics and possible application of these electrodes for cytarabine monitoring in different matrices. The electrodes are successfully applied to cytarabine determination in spiked biological fluid samples and in pharmaceutical formulations.https://www.mdpi.com/2073-4360/12/6/1343cytarabinemolecularly imprinted polymers (MIPs)potentiometric sensorsantileukemia drugmethod validationpharmaceutical formulations |
| spellingShingle | Ayman H. Kamel Abd El-Galil E. Amr Nashwa H. Ashmawy Hoda R. Galal Abdulrahman A. Almehizia Teraze A. Youssef Mohamed A. Al-Omar Ahmed Y. A. Sayed Validation of a Novel Potentiometric Method Based on a Polymeric PVC Membrane Sensor Integrated with Tailored Receptors for the Antileukemia Drug Cytarabine Polymers cytarabine molecularly imprinted polymers (MIPs) potentiometric sensors antileukemia drug method validation pharmaceutical formulations |
| title | Validation of a Novel Potentiometric Method Based on a Polymeric PVC Membrane Sensor Integrated with Tailored Receptors for the Antileukemia Drug Cytarabine |
| title_full | Validation of a Novel Potentiometric Method Based on a Polymeric PVC Membrane Sensor Integrated with Tailored Receptors for the Antileukemia Drug Cytarabine |
| title_fullStr | Validation of a Novel Potentiometric Method Based on a Polymeric PVC Membrane Sensor Integrated with Tailored Receptors for the Antileukemia Drug Cytarabine |
| title_full_unstemmed | Validation of a Novel Potentiometric Method Based on a Polymeric PVC Membrane Sensor Integrated with Tailored Receptors for the Antileukemia Drug Cytarabine |
| title_short | Validation of a Novel Potentiometric Method Based on a Polymeric PVC Membrane Sensor Integrated with Tailored Receptors for the Antileukemia Drug Cytarabine |
| title_sort | validation of a novel potentiometric method based on a polymeric pvc membrane sensor integrated with tailored receptors for the antileukemia drug cytarabine |
| topic | cytarabine molecularly imprinted polymers (MIPs) potentiometric sensors antileukemia drug method validation pharmaceutical formulations |
| url | https://www.mdpi.com/2073-4360/12/6/1343 |
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