The Use of Sugar Cane Waste (Molasses) in the Production of Lactic Acid by L. paracasei CAU 9836 and its Identification by Infrared Spectrum (FT.IR)
A total of twelve bacterial isolates from different samples of yoghurt and milk purchased from local markets in Diwaniyah Governorate. The preliminary screening was carried out all bacterial isolates by gram staining. The six isolates were selected based on the intensity of colour variation in the f...
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| Format: | Article |
| Language: | Arabic |
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University of Al-Qadisiya
2023-06-01
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| Series: | Al-Qadisiyah Journal For Agriculture Sciences |
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| Online Access: | https://jouagr.qu.edu.iq/article_177600_8930d2d5fd05faac861e0be6f63ff350.pdf |
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| author | باسم الخفاجی Adnan Al- Mudhafar basim alkhafaji |
| author_facet | باسم الخفاجی Adnan Al- Mudhafar basim alkhafaji |
| author_sort | باسم الخفاجی |
| collection | DOAJ |
| description | A total of twelve bacterial isolates from different samples of yoghurt and milk purchased from local markets in Diwaniyah Governorate. The preliminary screening was carried out all bacterial isolates by gram staining. The six isolates were selected based on the intensity of colour variation in the fermentation medium and were identified according to phenotypic, microscopic, and biochemical examinations, which included twelve isolates of Lactobacillus spp. Vitek II Compact instrument was used with all isolates to confirm the initial identification. The secondary screening was performed for isolates of Lactobacillus spp.species. Given the amount of the produced lactic acid, the L.casei strain 1859 had the highest lactic acid production, which was 64.32 g/L. The 16S rRNA test allowed the identification of this isolate.The results showed that the best conditions for acid production were 62.76 mg/L and the L.casei strain 9836 grown on the alternative medium containing 50 % whey, 7% molasses, and pH 6.9, incubated on an orbital shaker at 38°C for 24 hours, and agitated at 250 rpm.FTIR techniques were used to identify the nucleus of the hydrogen atom (proton) 1H and the carbon atoms 13C in the presence of standard lactic acid for comparison. The results revealed the peaks and bands of the produced acid were some similar to that of the standard acid. |
| first_indexed | 2024-03-13T08:10:54Z |
| format | Article |
| id | doaj.art-da2e6c30d5cc4031823cdf9e39938c21 |
| institution | Directory Open Access Journal |
| issn | 2077-5822 2618-1479 |
| language | Arabic |
| last_indexed | 2024-03-13T08:10:54Z |
| publishDate | 2023-06-01 |
| publisher | University of Al-Qadisiya |
| record_format | Article |
| series | Al-Qadisiyah Journal For Agriculture Sciences |
| spelling | doaj.art-da2e6c30d5cc4031823cdf9e39938c212023-05-31T21:21:56ZaraUniversity of Al-QadisiyaAl-Qadisiyah Journal For Agriculture Sciences2077-58222618-14792023-06-0113112313210.33794/qjas.2023.138240.1125177600The Use of Sugar Cane Waste (Molasses) in the Production of Lactic Acid by L. paracasei CAU 9836 and its Identification by Infrared Spectrum (FT.IR)باسم الخفاجی0Adnan Al- Mudhafar1basim alkhafaji2food science , agriculture college , university of kufaFood Science Department - Faculty Of Agriculture-University of Kufa Specialty: food science and Biotechnologyقسم علوم الاغذیة , کلیة الزراعة و جامعة الکوفة , العراقA total of twelve bacterial isolates from different samples of yoghurt and milk purchased from local markets in Diwaniyah Governorate. The preliminary screening was carried out all bacterial isolates by gram staining. The six isolates were selected based on the intensity of colour variation in the fermentation medium and were identified according to phenotypic, microscopic, and biochemical examinations, which included twelve isolates of Lactobacillus spp. Vitek II Compact instrument was used with all isolates to confirm the initial identification. The secondary screening was performed for isolates of Lactobacillus spp.species. Given the amount of the produced lactic acid, the L.casei strain 1859 had the highest lactic acid production, which was 64.32 g/L. The 16S rRNA test allowed the identification of this isolate.The results showed that the best conditions for acid production were 62.76 mg/L and the L.casei strain 9836 grown on the alternative medium containing 50 % whey, 7% molasses, and pH 6.9, incubated on an orbital shaker at 38°C for 24 hours, and agitated at 250 rpm.FTIR techniques were used to identify the nucleus of the hydrogen atom (proton) 1H and the carbon atoms 13C in the presence of standard lactic acid for comparison. The results revealed the peaks and bands of the produced acid were some similar to that of the standard acid.https://jouagr.qu.edu.iq/article_177600_8930d2d5fd05faac861e0be6f63ff350.pdfisolation,,,،,؛biochemical,,,،,؛fermentation,,,،,؛diagnosis |
| spellingShingle | باسم الخفاجی Adnan Al- Mudhafar basim alkhafaji The Use of Sugar Cane Waste (Molasses) in the Production of Lactic Acid by L. paracasei CAU 9836 and its Identification by Infrared Spectrum (FT.IR) Al-Qadisiyah Journal For Agriculture Sciences isolation,, ,،,؛biochemical,, ,،,؛fermentation,, ,،,؛diagnosis |
| title | The Use of Sugar Cane Waste (Molasses) in the Production of Lactic Acid by L. paracasei CAU 9836 and its Identification by Infrared Spectrum (FT.IR) |
| title_full | The Use of Sugar Cane Waste (Molasses) in the Production of Lactic Acid by L. paracasei CAU 9836 and its Identification by Infrared Spectrum (FT.IR) |
| title_fullStr | The Use of Sugar Cane Waste (Molasses) in the Production of Lactic Acid by L. paracasei CAU 9836 and its Identification by Infrared Spectrum (FT.IR) |
| title_full_unstemmed | The Use of Sugar Cane Waste (Molasses) in the Production of Lactic Acid by L. paracasei CAU 9836 and its Identification by Infrared Spectrum (FT.IR) |
| title_short | The Use of Sugar Cane Waste (Molasses) in the Production of Lactic Acid by L. paracasei CAU 9836 and its Identification by Infrared Spectrum (FT.IR) |
| title_sort | use of sugar cane waste molasses in the production of lactic acid by l paracasei cau 9836 and its identification by infrared spectrum ft ir |
| topic | isolation,, ,،,؛biochemical,, ,،,؛fermentation,, ,،,؛diagnosis |
| url | https://jouagr.qu.edu.iq/article_177600_8930d2d5fd05faac861e0be6f63ff350.pdf |
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