Effect of Exposure of Plastic Infant Feeding Bottle Leached Water on Biochemical, Morphological and Oxidative Stress Parameters in Rats
Bisphenol A (BPA) is leached out from plastic infant feeding bottles that are filled with warm milk/water due to high temperatures, exposing the infants to BPA. The present study aims to understand the effects of ingestion of BPA leached from plastic infant feeding bottle and delineate the underlyin...
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MDPI AG
2020-05-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/8/2/34 |
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| author | Mahendra K. Pant Abul H. Ahmad Manisha Naithani Hari S. Pandey Monika Pandey Jayanti Pant |
| author_facet | Mahendra K. Pant Abul H. Ahmad Manisha Naithani Hari S. Pandey Monika Pandey Jayanti Pant |
| author_sort | Mahendra K. Pant |
| collection | DOAJ |
| description | Bisphenol A (BPA) is leached out from plastic infant feeding bottles that are filled with warm milk/water due to high temperatures, exposing the infants to BPA. The present study aims to understand the effects of ingestion of BPA leached from plastic infant feeding bottle and delineate the underlying mechanisms in rats. In this study, adult rats of Wistar strain were divided into 3 groups. In the first group, the rats consumed normal food and tap water <i>ad libitum</i>. In the second group, the rats ingested BPA (20 µg/kg bodyweight/day, orally). In the third group, the rats drank water leached from plastic infant feeding bottles. After 30days, tests involving biochemical parameters, histopathological examination, and oxidative stress enzyme markers were performed, and the levels of BPA in plastic-leached water were estimated by HPLC analysis. There were significant biochemical changes in the form of increased alkaline phosphatase (ALP), creatine kinase-muscle/brain (CK-MB), and lactate dehydrogenase (LDH) levels in both treated groups as compared to control group, accompanied by structural damage to the vital organs, and lipid peroxidation, glutathione reductase, and catalase activity were also high in the treated groups. Further, the BPA concentration in plastic leached water was estimated to be 0.1 ± 0.02 µg/mL. |
| first_indexed | 2024-03-10T19:51:58Z |
| format | Article |
| id | doaj.art-da3134925f4e4e498bd0c7bc390ac9ec |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-10T19:51:58Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-da3134925f4e4e498bd0c7bc390ac9ec2023-11-20T00:18:31ZengMDPI AGToxics2305-63042020-05-01823410.3390/toxics8020034Effect of Exposure of Plastic Infant Feeding Bottle Leached Water on Biochemical, Morphological and Oxidative Stress Parameters in RatsMahendra K. Pant0Abul H. Ahmad1Manisha Naithani2Hari S. Pandey3Monika Pandey4Jayanti Pant5Department of Anatomy, Government Doon Medical College, Dehradun, Uttarakhand 248001, India; <email>pant.mahendra@gmail.com</email> Department of Veterinary Pharmacology & Toxicology, College of Veterinary & Animal Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263145, IndiaDepartment of Biochemistry, All India Institute of Medical Sciences, Rishikesh, Uttarakhand 249203, IndiaDepartment of Pathology, Government Doon Medical College, Dehradun, Uttarakhand 248001, India; <email>physianin@yahoo.com</email> Department of Physiology, Government Medical College, Haldwani, Uttarakhand 263139, IndiaDepartment of Physiology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand 249203, IndiaBisphenol A (BPA) is leached out from plastic infant feeding bottles that are filled with warm milk/water due to high temperatures, exposing the infants to BPA. The present study aims to understand the effects of ingestion of BPA leached from plastic infant feeding bottle and delineate the underlying mechanisms in rats. In this study, adult rats of Wistar strain were divided into 3 groups. In the first group, the rats consumed normal food and tap water <i>ad libitum</i>. In the second group, the rats ingested BPA (20 µg/kg bodyweight/day, orally). In the third group, the rats drank water leached from plastic infant feeding bottles. After 30days, tests involving biochemical parameters, histopathological examination, and oxidative stress enzyme markers were performed, and the levels of BPA in plastic-leached water were estimated by HPLC analysis. There were significant biochemical changes in the form of increased alkaline phosphatase (ALP), creatine kinase-muscle/brain (CK-MB), and lactate dehydrogenase (LDH) levels in both treated groups as compared to control group, accompanied by structural damage to the vital organs, and lipid peroxidation, glutathione reductase, and catalase activity were also high in the treated groups. Further, the BPA concentration in plastic leached water was estimated to be 0.1 ± 0.02 µg/mL.https://www.mdpi.com/2305-6304/8/2/34serum analysisBPAplastic leached wateroxidative stressinfant feeding bottles |
| spellingShingle | Mahendra K. Pant Abul H. Ahmad Manisha Naithani Hari S. Pandey Monika Pandey Jayanti Pant Effect of Exposure of Plastic Infant Feeding Bottle Leached Water on Biochemical, Morphological and Oxidative Stress Parameters in Rats Toxics serum analysis BPA plastic leached water oxidative stress infant feeding bottles |
| title | Effect of Exposure of Plastic Infant Feeding Bottle Leached Water on Biochemical, Morphological and Oxidative Stress Parameters in Rats |
| title_full | Effect of Exposure of Plastic Infant Feeding Bottle Leached Water on Biochemical, Morphological and Oxidative Stress Parameters in Rats |
| title_fullStr | Effect of Exposure of Plastic Infant Feeding Bottle Leached Water on Biochemical, Morphological and Oxidative Stress Parameters in Rats |
| title_full_unstemmed | Effect of Exposure of Plastic Infant Feeding Bottle Leached Water on Biochemical, Morphological and Oxidative Stress Parameters in Rats |
| title_short | Effect of Exposure of Plastic Infant Feeding Bottle Leached Water on Biochemical, Morphological and Oxidative Stress Parameters in Rats |
| title_sort | effect of exposure of plastic infant feeding bottle leached water on biochemical morphological and oxidative stress parameters in rats |
| topic | serum analysis BPA plastic leached water oxidative stress infant feeding bottles |
| url | https://www.mdpi.com/2305-6304/8/2/34 |
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