Modulating the surface potential of microspheres by phase transition in strontium doped barium titanate to restore the electric microenvironment for bone regeneration
The endogenous electrical potential generated by native bone and periosteum plays a key role in maintaining bone mass and quality. Inspired by the electrical properties of bone, different negative surface potentials are built on microspheres to restore electric microenvironment for powerful bone reg...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-08-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.988300/full |
| _version_ | 1817994645785804800 |
|---|---|
| author | Peng Wang Peng Wang Xiaosong Zhou Xiaosong Zhou Caili Lv Caili Lv Yu Wang Zongliang Wang Liqiang Wang Yongzhan Zhu Min Guo Peibiao Zhang Peibiao Zhang |
| author_facet | Peng Wang Peng Wang Xiaosong Zhou Xiaosong Zhou Caili Lv Caili Lv Yu Wang Zongliang Wang Liqiang Wang Yongzhan Zhu Min Guo Peibiao Zhang Peibiao Zhang |
| author_sort | Peng Wang |
| collection | DOAJ |
| description | The endogenous electrical potential generated by native bone and periosteum plays a key role in maintaining bone mass and quality. Inspired by the electrical properties of bone, different negative surface potentials are built on microspheres to restore electric microenvironment for powerful bone regeneration, which was prepared by the combination of strontium-doped barium titanate (Sr-BTO) nanoparticles and poly (lactic-co-glycolic acid) (PLGA) with high electrostatic voltage field (HEV). The surface potential was modulated through regulating the phase composition of nanoparticles in microspheres by the doping amount of strontium ion (Sr2+). As a result, the 0.1Sr-BTO/PLGA group shows the lowest surface potential and its relative permittivity is closer to natural bone. As expected, the 0.1Sr-BTO/PLGA microspheres performed cytocompatibility, osteogenic activity in vitro and enhance bone regeneration in vivo. Furthermore, the potential mechanism of Sr-BTO/PLGA microspheres to promote osteogenic differentiation was further explored. The lower surface potential generated on Sr-BTO/PLGA microspheres regulates cell membrane potential and leads to an increase in the intracellular calcium ion (Ca2+) concentration, which could activate the Calcineurin (CaN)/Nuclear factor of activated T-cells (NFAT) signaling pathway to promote osteogenic differentiation. This study established an effective method to modulate the surface potential, which provides a prospective exploration for electrical stimulation therapy. The 0.1Sr-BTO/PLGA microsphere with lower surface potential and bone-matched dielectric constant is expected to have great potential in the field of bone regeneration. |
| first_indexed | 2024-04-14T01:54:48Z |
| format | Article |
| id | doaj.art-da3495ca01764003bbc5a0db533d6b1e |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-14T01:54:48Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-da3495ca01764003bbc5a0db533d6b1e2022-12-22T02:19:08ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-08-011010.3389/fbioe.2022.988300988300Modulating the surface potential of microspheres by phase transition in strontium doped barium titanate to restore the electric microenvironment for bone regenerationPeng Wang0Peng Wang1Xiaosong Zhou2Xiaosong Zhou3Caili Lv4Caili Lv5Yu Wang6Zongliang Wang7Liqiang Wang8Yongzhan Zhu9Min Guo10Peibiao Zhang11Peibiao Zhang12Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, ChinaSchool of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, ChinaKey Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, ChinaSchool of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, ChinaKey Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, ChinaSchool of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, ChinaKey Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, ChinaKey Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, ChinaDepartment of Ophthalmology, Third Medical Center, Chinese PLA General Hospital, Beijing, China8th Department of Orthopaedics, Foshan Hospital of Traditional Chinese Medicine, Foshan, ChinaKey Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, ChinaKey Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, ChinaSchool of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, ChinaThe endogenous electrical potential generated by native bone and periosteum plays a key role in maintaining bone mass and quality. Inspired by the electrical properties of bone, different negative surface potentials are built on microspheres to restore electric microenvironment for powerful bone regeneration, which was prepared by the combination of strontium-doped barium titanate (Sr-BTO) nanoparticles and poly (lactic-co-glycolic acid) (PLGA) with high electrostatic voltage field (HEV). The surface potential was modulated through regulating the phase composition of nanoparticles in microspheres by the doping amount of strontium ion (Sr2+). As a result, the 0.1Sr-BTO/PLGA group shows the lowest surface potential and its relative permittivity is closer to natural bone. As expected, the 0.1Sr-BTO/PLGA microspheres performed cytocompatibility, osteogenic activity in vitro and enhance bone regeneration in vivo. Furthermore, the potential mechanism of Sr-BTO/PLGA microspheres to promote osteogenic differentiation was further explored. The lower surface potential generated on Sr-BTO/PLGA microspheres regulates cell membrane potential and leads to an increase in the intracellular calcium ion (Ca2+) concentration, which could activate the Calcineurin (CaN)/Nuclear factor of activated T-cells (NFAT) signaling pathway to promote osteogenic differentiation. This study established an effective method to modulate the surface potential, which provides a prospective exploration for electrical stimulation therapy. The 0.1Sr-BTO/PLGA microsphere with lower surface potential and bone-matched dielectric constant is expected to have great potential in the field of bone regeneration.https://www.frontiersin.org/articles/10.3389/fbioe.2022.988300/fullmicrospheresurface potentialdielectric propertiesbone regenerationdopedbarium titanate |
| spellingShingle | Peng Wang Peng Wang Xiaosong Zhou Xiaosong Zhou Caili Lv Caili Lv Yu Wang Zongliang Wang Liqiang Wang Yongzhan Zhu Min Guo Peibiao Zhang Peibiao Zhang Modulating the surface potential of microspheres by phase transition in strontium doped barium titanate to restore the electric microenvironment for bone regeneration Frontiers in Bioengineering and Biotechnology microsphere surface potential dielectric properties bone regeneration doped barium titanate |
| title | Modulating the surface potential of microspheres by phase transition in strontium doped barium titanate to restore the electric microenvironment for bone regeneration |
| title_full | Modulating the surface potential of microspheres by phase transition in strontium doped barium titanate to restore the electric microenvironment for bone regeneration |
| title_fullStr | Modulating the surface potential of microspheres by phase transition in strontium doped barium titanate to restore the electric microenvironment for bone regeneration |
| title_full_unstemmed | Modulating the surface potential of microspheres by phase transition in strontium doped barium titanate to restore the electric microenvironment for bone regeneration |
| title_short | Modulating the surface potential of microspheres by phase transition in strontium doped barium titanate to restore the electric microenvironment for bone regeneration |
| title_sort | modulating the surface potential of microspheres by phase transition in strontium doped barium titanate to restore the electric microenvironment for bone regeneration |
| topic | microsphere surface potential dielectric properties bone regeneration doped barium titanate |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.988300/full |
| work_keys_str_mv | AT pengwang modulatingthesurfacepotentialofmicrospheresbyphasetransitioninstrontiumdopedbariumtitanatetorestoretheelectricmicroenvironmentforboneregeneration AT pengwang modulatingthesurfacepotentialofmicrospheresbyphasetransitioninstrontiumdopedbariumtitanatetorestoretheelectricmicroenvironmentforboneregeneration AT xiaosongzhou modulatingthesurfacepotentialofmicrospheresbyphasetransitioninstrontiumdopedbariumtitanatetorestoretheelectricmicroenvironmentforboneregeneration AT xiaosongzhou modulatingthesurfacepotentialofmicrospheresbyphasetransitioninstrontiumdopedbariumtitanatetorestoretheelectricmicroenvironmentforboneregeneration AT caililv modulatingthesurfacepotentialofmicrospheresbyphasetransitioninstrontiumdopedbariumtitanatetorestoretheelectricmicroenvironmentforboneregeneration AT caililv modulatingthesurfacepotentialofmicrospheresbyphasetransitioninstrontiumdopedbariumtitanatetorestoretheelectricmicroenvironmentforboneregeneration AT yuwang modulatingthesurfacepotentialofmicrospheresbyphasetransitioninstrontiumdopedbariumtitanatetorestoretheelectricmicroenvironmentforboneregeneration AT zongliangwang modulatingthesurfacepotentialofmicrospheresbyphasetransitioninstrontiumdopedbariumtitanatetorestoretheelectricmicroenvironmentforboneregeneration AT liqiangwang modulatingthesurfacepotentialofmicrospheresbyphasetransitioninstrontiumdopedbariumtitanatetorestoretheelectricmicroenvironmentforboneregeneration AT yongzhanzhu modulatingthesurfacepotentialofmicrospheresbyphasetransitioninstrontiumdopedbariumtitanatetorestoretheelectricmicroenvironmentforboneregeneration AT minguo modulatingthesurfacepotentialofmicrospheresbyphasetransitioninstrontiumdopedbariumtitanatetorestoretheelectricmicroenvironmentforboneregeneration AT peibiaozhang modulatingthesurfacepotentialofmicrospheresbyphasetransitioninstrontiumdopedbariumtitanatetorestoretheelectricmicroenvironmentforboneregeneration AT peibiaozhang modulatingthesurfacepotentialofmicrospheresbyphasetransitioninstrontiumdopedbariumtitanatetorestoretheelectricmicroenvironmentforboneregeneration |