Ultrasound-Assisted Extraction of Anthocyanins from Haskap (Lonicera caerulea L.) Berries Using a Deep Eutectic Solvent (DES) DES Extraction of Anthocyanins from Haskap Berries
Research background. Haskap berries are one of the richest natural sources of anthocyanins and their extracts can be used for nutraceuticals and functional food ingredients. Deep eutectic solvents (DES) comprising food-grade or generally recognized as safe (GRAS) components show promise as natural s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2021-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | https://hrcak.srce.hr/file/373414 |
| _version_ | 1797207031140057088 |
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| author | Amanda M.G. MacLean Yasmini P.A. Silva Guangling Jiao Marianne S. Brooks |
| author_facet | Amanda M.G. MacLean Yasmini P.A. Silva Guangling Jiao Marianne S. Brooks |
| author_sort | Amanda M.G. MacLean |
| collection | DOAJ |
| description | Research background. Haskap berries are one of the richest natural sources of anthocyanins and their extracts can be used for nutraceuticals and functional food ingredients. Deep eutectic solvents (DES) comprising food-grade or generally recognized as safe (GRAS) components show promise as natural solvents, but have not been applied to haskap berries. Thus, the aim of this study is to investigate the extraction of anthocyanins from haskap berries using a DES consisting of citric acid and d-(+)-maltose.
Experimental approach. The experimental approach used ultrasound-assisted extraction with a DES consisting of citric acid and d-(+)-maltose as the solvent to achieve a sustainable green extraction process. Response surface methodology (RSM) with a Box-Behnken experimental design was used to study the effect of varying the extraction temperature, time of extraction, V(solvent)/m(sample) ratio (mL/g) and the water volume fraction (%) in the DES on the total anthocyanin content (TAC) in the haskap berry extracts.
Results and conclusions. Under the optimal extraction conditions (75 °C, 10 min, 50.4 mL/g and 90% water) a predicted TAC extraction on dry mass basis yielded 21.2 mg/g, with experimental error of 7.2%. The TAC yield and anthocyanin profiles were similar to those obtained with conventional organic solvents.
Novelty and scientific contribution. This is the first study investigating the use of a food-grade DES comprising GRAS components for the extraction of anthocyanins from haskap berries. These results indicate that the studied DES (citric acid and d-(+)-maltose) is a suitable alternative solvent for extracting anthocyanins for food-grade applications. |
| first_indexed | 2024-04-24T09:16:26Z |
| format | Article |
| id | doaj.art-da3850315d5f4f6fa9e362550add378c |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-04-24T09:16:26Z |
| publishDate | 2021-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-da3850315d5f4f6fa9e362550add378c2024-04-15T16:54:52ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062021-01-01591566210.17113/ftb.59.01.21.6869Ultrasound-Assisted Extraction of Anthocyanins from Haskap (Lonicera caerulea L.) Berries Using a Deep Eutectic Solvent (DES) DES Extraction of Anthocyanins from Haskap BerriesAmanda M.G. MacLean0Yasmini P.A. Silva1Guangling Jiao2Marianne S. Brooks3Department of Process Engineering and Applied Science, Dalhousie University, 5273 DaCosta Row, PO Box 15000, Halifax, NS, B3H 4R2, CanadaFaculty of Nutrition, University of Goiás, Rua 227, qd. 68, Setor Leste Universitário, Goiânia, GO, 74605-080, BrazilDepartment of Process Engineering and Applied Science, Dalhousie University, 5273 DaCosta Row, PO Box 15000, Halifax, NS, B3H 4R2, CanadaDepartment of Process Engineering and Applied Science, Dalhousie University, 5273 DaCosta Row, PO Box 15000, Halifax, NS, B3H 4R2, CanadaResearch background. Haskap berries are one of the richest natural sources of anthocyanins and their extracts can be used for nutraceuticals and functional food ingredients. Deep eutectic solvents (DES) comprising food-grade or generally recognized as safe (GRAS) components show promise as natural solvents, but have not been applied to haskap berries. Thus, the aim of this study is to investigate the extraction of anthocyanins from haskap berries using a DES consisting of citric acid and d-(+)-maltose. Experimental approach. The experimental approach used ultrasound-assisted extraction with a DES consisting of citric acid and d-(+)-maltose as the solvent to achieve a sustainable green extraction process. Response surface methodology (RSM) with a Box-Behnken experimental design was used to study the effect of varying the extraction temperature, time of extraction, V(solvent)/m(sample) ratio (mL/g) and the water volume fraction (%) in the DES on the total anthocyanin content (TAC) in the haskap berry extracts. Results and conclusions. Under the optimal extraction conditions (75 °C, 10 min, 50.4 mL/g and 90% water) a predicted TAC extraction on dry mass basis yielded 21.2 mg/g, with experimental error of 7.2%. The TAC yield and anthocyanin profiles were similar to those obtained with conventional organic solvents. Novelty and scientific contribution. This is the first study investigating the use of a food-grade DES comprising GRAS components for the extraction of anthocyanins from haskap berries. These results indicate that the studied DES (citric acid and d-(+)-maltose) is a suitable alternative solvent for extracting anthocyanins for food-grade applications.https://hrcak.srce.hr/file/373414anthocyaninsBox-Behnken designdeep eutectic solventgreen extractionhaskap |
| spellingShingle | Amanda M.G. MacLean Yasmini P.A. Silva Guangling Jiao Marianne S. Brooks Ultrasound-Assisted Extraction of Anthocyanins from Haskap (Lonicera caerulea L.) Berries Using a Deep Eutectic Solvent (DES) DES Extraction of Anthocyanins from Haskap Berries Food Technology and Biotechnology anthocyanins Box-Behnken design deep eutectic solvent green extraction haskap |
| title | Ultrasound-Assisted Extraction of Anthocyanins from Haskap (Lonicera caerulea L.) Berries Using a Deep Eutectic Solvent (DES) DES Extraction of Anthocyanins from Haskap Berries |
| title_full | Ultrasound-Assisted Extraction of Anthocyanins from Haskap (Lonicera caerulea L.) Berries Using a Deep Eutectic Solvent (DES) DES Extraction of Anthocyanins from Haskap Berries |
| title_fullStr | Ultrasound-Assisted Extraction of Anthocyanins from Haskap (Lonicera caerulea L.) Berries Using a Deep Eutectic Solvent (DES) DES Extraction of Anthocyanins from Haskap Berries |
| title_full_unstemmed | Ultrasound-Assisted Extraction of Anthocyanins from Haskap (Lonicera caerulea L.) Berries Using a Deep Eutectic Solvent (DES) DES Extraction of Anthocyanins from Haskap Berries |
| title_short | Ultrasound-Assisted Extraction of Anthocyanins from Haskap (Lonicera caerulea L.) Berries Using a Deep Eutectic Solvent (DES) DES Extraction of Anthocyanins from Haskap Berries |
| title_sort | ultrasound assisted extraction of anthocyanins from haskap lonicera caerulea l berries using a deep eutectic solvent des des extraction of anthocyanins from haskap berries |
| topic | anthocyanins Box-Behnken design deep eutectic solvent green extraction haskap |
| url | https://hrcak.srce.hr/file/373414 |
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