Chlorpyrifos-induced Histopathological Alterations in Duttaphrynus melanostictus (Schneider 1799) Tadpoles: Evidence from Empirical Trials
This paper reports the histopathological responses of the gill, liver and tail muscle tissues in tadpoles of the Asian Common Toad Duttaphrynus melanostictus (Schneider, 1799) exposed to chlorpyrifos a common organophosphorus pesticide. Tadpoles of Gosner stages 24-26 were continuously exposed to lo...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Sri Jayewardenepura
2012-10-01
|
| Series: | Journal of Tropical Forestry and Environment |
| Online Access: | http://journals.sjp.ac.lk/index.php/JTFE/article/view/592 |
| _version_ | 1828795961010290688 |
|---|---|
| author | M. G. D. K. Bandara M.R. Wijesinghe W. D. Ratnasooriya A. A. H. Priyani |
| author_facet | M. G. D. K. Bandara M.R. Wijesinghe W. D. Ratnasooriya A. A. H. Priyani |
| author_sort | M. G. D. K. Bandara |
| collection | DOAJ |
| description | This paper reports the histopathological responses of the gill, liver and tail muscle tissues in tadpoles of the Asian Common Toad Duttaphrynus melanostictus (Schneider, 1799) exposed to chlorpyrifos a common organophosphorus pesticide. Tadpoles of Gosner stages 24-26 were continuously exposed to low, mid and high (500, 1000 and 1500 µgl–1) concentrations of chlorpyrifos for two weeks. Histological alterations in the tissues of the surviving larvae were microscopically examined both at the end of the exposure period and after a week following the final exposure. Several histological alterations were noted in the gills, liver and tail muscles of the larvae exposed to 1500 µgl–1 of chlorpyrifos. The gills of exposed larvae showed architectural distortion resulting from reduced primary and secondary gill lamellae and blood vessels, and alterations in the gill epithelium. In the liver sinusoidal congestion and dilation, cytoplasmic vacuolation and changes in hepatocytes such as hyperchromatic nuclei and nuclear fragmentation were observed. The tail muscle tissue suffered from severe atrophy and myotomal disintegration. Although histological alterations in the gill and liver tissues were noted only at the high concentration, changes in the muscle tissue i.e. muscle degeneration and atrophy, were apparent at both low and mid concentrations. The degree of damage in surviving larvae in a week following the final exposure was lower than that observed during the exposure, probably indicating recovery or resistance. The findings of the present study emphasize the need to investigate possible sublethal damage induced by pesticides in amphibians inhabiting agricultural habitats. |
| first_indexed | 2024-12-12T04:14:01Z |
| format | Article |
| id | doaj.art-da3ac3d36a964be1959321ce88e403f9 |
| institution | Directory Open Access Journal |
| issn | 2235-9370 2235-9362 |
| language | English |
| last_indexed | 2024-12-12T04:14:01Z |
| publishDate | 2012-10-01 |
| publisher | University of Sri Jayewardenepura |
| record_format | Article |
| series | Journal of Tropical Forestry and Environment |
| spelling | doaj.art-da3ac3d36a964be1959321ce88e403f92022-12-22T00:38:32ZengUniversity of Sri JayewardenepuraJournal of Tropical Forestry and Environment2235-93702235-93622012-10-0122498Chlorpyrifos-induced Histopathological Alterations in Duttaphrynus melanostictus (Schneider 1799) Tadpoles: Evidence from Empirical TrialsM. G. D. K. Bandara0M.R. Wijesinghe1W. D. Ratnasooriya2A. A. H. Priyani3Department of Zoology, Faculty of Science, University of Colombo, Colombo 03, Sri LankaDepartment of Zoology, Faculty of Science, University of Colombo, Colombo 03, Sri LankaDepartment of Zoology, Faculty of Science, University of Colombo, Colombo 03, Sri LankaDepartment of Pathology, Faculty of Medicine, University of Colombo, Colombo 03, Sri LankaThis paper reports the histopathological responses of the gill, liver and tail muscle tissues in tadpoles of the Asian Common Toad Duttaphrynus melanostictus (Schneider, 1799) exposed to chlorpyrifos a common organophosphorus pesticide. Tadpoles of Gosner stages 24-26 were continuously exposed to low, mid and high (500, 1000 and 1500 µgl–1) concentrations of chlorpyrifos for two weeks. Histological alterations in the tissues of the surviving larvae were microscopically examined both at the end of the exposure period and after a week following the final exposure. Several histological alterations were noted in the gills, liver and tail muscles of the larvae exposed to 1500 µgl–1 of chlorpyrifos. The gills of exposed larvae showed architectural distortion resulting from reduced primary and secondary gill lamellae and blood vessels, and alterations in the gill epithelium. In the liver sinusoidal congestion and dilation, cytoplasmic vacuolation and changes in hepatocytes such as hyperchromatic nuclei and nuclear fragmentation were observed. The tail muscle tissue suffered from severe atrophy and myotomal disintegration. Although histological alterations in the gill and liver tissues were noted only at the high concentration, changes in the muscle tissue i.e. muscle degeneration and atrophy, were apparent at both low and mid concentrations. The degree of damage in surviving larvae in a week following the final exposure was lower than that observed during the exposure, probably indicating recovery or resistance. The findings of the present study emphasize the need to investigate possible sublethal damage induced by pesticides in amphibians inhabiting agricultural habitats.http://journals.sjp.ac.lk/index.php/JTFE/article/view/592 |
| spellingShingle | M. G. D. K. Bandara M.R. Wijesinghe W. D. Ratnasooriya A. A. H. Priyani Chlorpyrifos-induced Histopathological Alterations in Duttaphrynus melanostictus (Schneider 1799) Tadpoles: Evidence from Empirical Trials Journal of Tropical Forestry and Environment |
| title | Chlorpyrifos-induced Histopathological Alterations in Duttaphrynus melanostictus (Schneider 1799) Tadpoles: Evidence from Empirical Trials |
| title_full | Chlorpyrifos-induced Histopathological Alterations in Duttaphrynus melanostictus (Schneider 1799) Tadpoles: Evidence from Empirical Trials |
| title_fullStr | Chlorpyrifos-induced Histopathological Alterations in Duttaphrynus melanostictus (Schneider 1799) Tadpoles: Evidence from Empirical Trials |
| title_full_unstemmed | Chlorpyrifos-induced Histopathological Alterations in Duttaphrynus melanostictus (Schneider 1799) Tadpoles: Evidence from Empirical Trials |
| title_short | Chlorpyrifos-induced Histopathological Alterations in Duttaphrynus melanostictus (Schneider 1799) Tadpoles: Evidence from Empirical Trials |
| title_sort | chlorpyrifos induced histopathological alterations in duttaphrynus melanostictus schneider 1799 tadpoles evidence from empirical trials |
| url | http://journals.sjp.ac.lk/index.php/JTFE/article/view/592 |
| work_keys_str_mv | AT mgdkbandara chlorpyrifosinducedhistopathologicalalterationsinduttaphrynusmelanostictusschneider1799tadpolesevidencefromempiricaltrials AT mrwijesinghe chlorpyrifosinducedhistopathologicalalterationsinduttaphrynusmelanostictusschneider1799tadpolesevidencefromempiricaltrials AT wdratnasooriya chlorpyrifosinducedhistopathologicalalterationsinduttaphrynusmelanostictusschneider1799tadpolesevidencefromempiricaltrials AT aahpriyani chlorpyrifosinducedhistopathologicalalterationsinduttaphrynusmelanostictusschneider1799tadpolesevidencefromempiricaltrials |