Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China
Corallorhiza sinensis, a new species of mycoheterotrophic orchid from western Sichuan, China, is described and illustrated based on molecular and morphological evidence. It is morphologically similar to Corallorhiza trifida, but can be distinguished by bigger flowers, both sepals and petals with 3 v...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2021-10-01
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| Series: | Plant Diversity |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468265921000020 |
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| author | Jia-Xin Yang Shuai Peng Jun-Jie Wang Shi-Xiong Ding Yan Wang Jing Tian Han Yang Guang-Wan Hu Qing-Feng Wang |
| author_facet | Jia-Xin Yang Shuai Peng Jun-Jie Wang Shi-Xiong Ding Yan Wang Jing Tian Han Yang Guang-Wan Hu Qing-Feng Wang |
| author_sort | Jia-Xin Yang |
| collection | DOAJ |
| description | Corallorhiza sinensis, a new species of mycoheterotrophic orchid from western Sichuan, China, is described and illustrated based on molecular and morphological evidence. It is morphologically similar to Corallorhiza trifida, but can be distinguished by bigger flowers, both sepals and petals with 3 veins, and longer lateral lobes of lip. To distinguish the new Corallorhiza species and explore its phylogenetic position within subtribe Calypsoinae, this study employed sequences of the nuclear ribosomal DNA (nrDNA) and whole plastome assembled from the genome skimming approach. The plastome is 148,124 bp in length, including a pair of inverted repeats (IRs) of 26,165 bp, a large single-copy region (LSC) of 82,207 bp, and a small single-copy region (SSC) of 13,587 bp. Further, phylogenetic analyses were performed using nrDNA sequence and 79 coding sequences (CDSs) from 26 complete plastomes of subtribe Calypsoinae. The phylogenetic tree based on nrDNA sequence suggested that Corallorhiza is a monophyletic group, and strongly support C. sinensis as sister to the rest species of Corallorhiza. The plastid tree showed that 10 Corallorhiza species grouped into two clades and C. sinensis is most closely related to the North American C. striata and C. bentleyi instead of Oreorchis foliosa and O. angustata in the same clade. The plastid tree and nrDNA tree indicate Oreorchis is a paraphyletic. Although the topological conflicts are displayed between plastome and nrDNA phylogenies of C. sinensis, it is still the most closely related to Corallorhiza. Comparative analysis showed that C. sinensis populations are characteristic of the intermediate morphological traits between Corallorhiza and Oreorchis. The finding of this new species from China shed new light on the phylogeny of Oreorchis and Corallorhiza. |
| first_indexed | 2024-12-23T19:21:11Z |
| format | Article |
| id | doaj.art-da3e2640c13242b3a3ca8cd45bc98c89 |
| institution | Directory Open Access Journal |
| issn | 2468-2659 |
| language | English |
| last_indexed | 2024-12-23T19:21:11Z |
| publishDate | 2021-10-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Plant Diversity |
| spelling | doaj.art-da3e2640c13242b3a3ca8cd45bc98c892022-12-21T17:34:10ZengKeAi Communications Co., Ltd.Plant Diversity2468-26592021-10-01435409419Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW ChinaJia-Xin Yang0Shuai Peng1Jun-Jie Wang2Shi-Xiong Ding3Yan Wang4Jing Tian5Han Yang6Guang-Wan Hu7Qing-Feng Wang8CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430074, China; University of Chinese Academy of Sciences, Beijing, 100049, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430074, China; University of Chinese Academy of Sciences, Beijing, 100049, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430074, China; University of Chinese Academy of Sciences, Beijing, 100049, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430074, China; Jiangxi Provincial Key Laboratory for Bamboo Germplasm Resources and Utilization, Forestry College, Jiangxi Agricultural University, Nanchang, 330045, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430074, China; University of Chinese Academy of Sciences, Beijing, 100049, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430074, ChinaAdministration of Siguniang Mountains National Nature Reserve, Xiaojin, 624206, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430074, China; Corresponding author. CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China.CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430074, ChinaCorallorhiza sinensis, a new species of mycoheterotrophic orchid from western Sichuan, China, is described and illustrated based on molecular and morphological evidence. It is morphologically similar to Corallorhiza trifida, but can be distinguished by bigger flowers, both sepals and petals with 3 veins, and longer lateral lobes of lip. To distinguish the new Corallorhiza species and explore its phylogenetic position within subtribe Calypsoinae, this study employed sequences of the nuclear ribosomal DNA (nrDNA) and whole plastome assembled from the genome skimming approach. The plastome is 148,124 bp in length, including a pair of inverted repeats (IRs) of 26,165 bp, a large single-copy region (LSC) of 82,207 bp, and a small single-copy region (SSC) of 13,587 bp. Further, phylogenetic analyses were performed using nrDNA sequence and 79 coding sequences (CDSs) from 26 complete plastomes of subtribe Calypsoinae. The phylogenetic tree based on nrDNA sequence suggested that Corallorhiza is a monophyletic group, and strongly support C. sinensis as sister to the rest species of Corallorhiza. The plastid tree showed that 10 Corallorhiza species grouped into two clades and C. sinensis is most closely related to the North American C. striata and C. bentleyi instead of Oreorchis foliosa and O. angustata in the same clade. The plastid tree and nrDNA tree indicate Oreorchis is a paraphyletic. Although the topological conflicts are displayed between plastome and nrDNA phylogenies of C. sinensis, it is still the most closely related to Corallorhiza. Comparative analysis showed that C. sinensis populations are characteristic of the intermediate morphological traits between Corallorhiza and Oreorchis. The finding of this new species from China shed new light on the phylogeny of Oreorchis and Corallorhiza.http://www.sciencedirect.com/science/article/pii/S2468265921000020Corallorhiza sinensisOreorchisSichuan provincePlastomePhylogeny |
| spellingShingle | Jia-Xin Yang Shuai Peng Jun-Jie Wang Shi-Xiong Ding Yan Wang Jing Tian Han Yang Guang-Wan Hu Qing-Feng Wang Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China Plant Diversity Corallorhiza sinensis Oreorchis Sichuan province Plastome Phylogeny |
| title | Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China |
| title_full | Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China |
| title_fullStr | Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China |
| title_full_unstemmed | Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China |
| title_short | Morphological and genomic evidence for a new species of Corallorhiza (Orchidaceae: Epidendroideae) from SW China |
| title_sort | morphological and genomic evidence for a new species of corallorhiza orchidaceae epidendroideae from sw china |
| topic | Corallorhiza sinensis Oreorchis Sichuan province Plastome Phylogeny |
| url | http://www.sciencedirect.com/science/article/pii/S2468265921000020 |
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