Disruptions in Hypothalamic–Pituitary–Gonadal Axis Development and Their IgG Modulation after Prenatal Systemic Inflammation in Male Rats
The development of the neuroendocrine system, including the hypothalamic–pituitary–gonadal (HPG) axis, is sensitive to environmental impacts during critical developmental periods. Maternal immune system activation by bacterial or viral infection may be one of the negative impacts. This study focused...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2726 |
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| author | Vasilina Ignatiuk Marina Izvolskaia Viktoria Sharova Liudmila Zakharova |
| author_facet | Vasilina Ignatiuk Marina Izvolskaia Viktoria Sharova Liudmila Zakharova |
| author_sort | Vasilina Ignatiuk |
| collection | DOAJ |
| description | The development of the neuroendocrine system, including the hypothalamic–pituitary–gonadal (HPG) axis, is sensitive to environmental impacts during critical developmental periods. Maternal immune system activation by bacterial or viral infection may be one of the negative impacts. This study focused on the effect of systemic inflammation induced by lipopolysaccharides (LPS <i>E. coli</i>) on the HPG axis development in male rat offspring, corrected by the anti-inflammatory action of polyclonal IgG and monoclonal anti-interleukin (IL)-6 receptor antibodies (IL-6RmAbs). A single LPS exposure on the 12th embryonic day (ED) led to a decrease in the number of afferent synaptic inputs on gonadotropin-releasing, hormone-producing neurons in adult male offspring. LPS exposure on ED18 did not lead to such disruptions. Moreover, after the LPS injections on ED12, circulating follicle-stimulating hormone and sex steroid levels were reduced, and the gonadal structure was disrupted. A prenatal IL-6R blockade with IL-6RmAbs and polyclonal IgG reduced the negative effects of inflammation on fetal HPG axis development. Overall, the data obtained confirm the morphogenetic effect of inflammation on fetal HPG development and IL-6 involvement in these processes. |
| first_indexed | 2024-03-11T09:41:17Z |
| format | Article |
| id | doaj.art-da412ab75de34427909e15ad3bdea300 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:41:17Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-da412ab75de34427909e15ad3bdea3002023-11-16T17:01:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01243272610.3390/ijms24032726Disruptions in Hypothalamic–Pituitary–Gonadal Axis Development and Their IgG Modulation after Prenatal Systemic Inflammation in Male RatsVasilina Ignatiuk0Marina Izvolskaia1Viktoria Sharova2Liudmila Zakharova3Koltsov Institute of Developmental Biology, The Russian Academy of Sciences, Vavilov Street, 26, 119334 Moscow, RussiaKoltsov Institute of Developmental Biology, The Russian Academy of Sciences, Vavilov Street, 26, 119334 Moscow, RussiaKoltsov Institute of Developmental Biology, The Russian Academy of Sciences, Vavilov Street, 26, 119334 Moscow, RussiaKoltsov Institute of Developmental Biology, The Russian Academy of Sciences, Vavilov Street, 26, 119334 Moscow, RussiaThe development of the neuroendocrine system, including the hypothalamic–pituitary–gonadal (HPG) axis, is sensitive to environmental impacts during critical developmental periods. Maternal immune system activation by bacterial or viral infection may be one of the negative impacts. This study focused on the effect of systemic inflammation induced by lipopolysaccharides (LPS <i>E. coli</i>) on the HPG axis development in male rat offspring, corrected by the anti-inflammatory action of polyclonal IgG and monoclonal anti-interleukin (IL)-6 receptor antibodies (IL-6RmAbs). A single LPS exposure on the 12th embryonic day (ED) led to a decrease in the number of afferent synaptic inputs on gonadotropin-releasing, hormone-producing neurons in adult male offspring. LPS exposure on ED18 did not lead to such disruptions. Moreover, after the LPS injections on ED12, circulating follicle-stimulating hormone and sex steroid levels were reduced, and the gonadal structure was disrupted. A prenatal IL-6R blockade with IL-6RmAbs and polyclonal IgG reduced the negative effects of inflammation on fetal HPG axis development. Overall, the data obtained confirm the morphogenetic effect of inflammation on fetal HPG development and IL-6 involvement in these processes.https://www.mdpi.com/1422-0067/24/3/2726prenatal inflammationHPG axisGnRH neuronscritical periods of developmentlong-term effectsIL-6 |
| spellingShingle | Vasilina Ignatiuk Marina Izvolskaia Viktoria Sharova Liudmila Zakharova Disruptions in Hypothalamic–Pituitary–Gonadal Axis Development and Their IgG Modulation after Prenatal Systemic Inflammation in Male Rats International Journal of Molecular Sciences prenatal inflammation HPG axis GnRH neurons critical periods of development long-term effects IL-6 |
| title | Disruptions in Hypothalamic–Pituitary–Gonadal Axis Development and Their IgG Modulation after Prenatal Systemic Inflammation in Male Rats |
| title_full | Disruptions in Hypothalamic–Pituitary–Gonadal Axis Development and Their IgG Modulation after Prenatal Systemic Inflammation in Male Rats |
| title_fullStr | Disruptions in Hypothalamic–Pituitary–Gonadal Axis Development and Their IgG Modulation after Prenatal Systemic Inflammation in Male Rats |
| title_full_unstemmed | Disruptions in Hypothalamic–Pituitary–Gonadal Axis Development and Their IgG Modulation after Prenatal Systemic Inflammation in Male Rats |
| title_short | Disruptions in Hypothalamic–Pituitary–Gonadal Axis Development and Their IgG Modulation after Prenatal Systemic Inflammation in Male Rats |
| title_sort | disruptions in hypothalamic pituitary gonadal axis development and their igg modulation after prenatal systemic inflammation in male rats |
| topic | prenatal inflammation HPG axis GnRH neurons critical periods of development long-term effects IL-6 |
| url | https://www.mdpi.com/1422-0067/24/3/2726 |
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