CircRNA expression profiles in decidual tissue of patients with early recurrent miscarriage
Circular RNAs (circRNAs) are a novel class of endogenous noncoding RNAs that play important roles in gene expression regulation. This study aimed to evaluate the potential role of circRNAs in decidual tissue of patients with early recurrent miscarriage (RM). We constructed circRNA expression profile...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2020-09-01
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| Series: | Genes and Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235230421930039X |
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| author | Chunli Li Xuemei Chen Xueqing Liu Xiaoli Liu Junlin He Yubin Ding Chao Tong Chuan Peng Yingxiong Wang Rufei Gao |
| author_facet | Chunli Li Xuemei Chen Xueqing Liu Xiaoli Liu Junlin He Yubin Ding Chao Tong Chuan Peng Yingxiong Wang Rufei Gao |
| author_sort | Chunli Li |
| collection | DOAJ |
| description | Circular RNAs (circRNAs) are a novel class of endogenous noncoding RNAs that play important roles in gene expression regulation. This study aimed to evaluate the potential role of circRNAs in decidual tissue of patients with early recurrent miscarriage (RM). We constructed circRNA expression profiles in decidual tissue using microarray data. A total of 123 differentially expressed circRNAs, including 78 upregulated and 45 downregulated circRNAs were detected in the early RM group compared with the control group (P < 0.05). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis also revealed the enrichment of specific circRNAs. The verified circRNA-targeted miRNA-mRNA network was constructed, most of the circRNAs harbored miRNA binding sites. The network involved 3 circRNAs, 27 microRNAs and 82 mRNAs. Hsa_circRNA_103092- miR-224-PRLR network was selected to verify by qRT-PCR. These results showed that circRNAs are aberrantly expressed in the decidual tissue in early RM patients and play potential roles in the development of early RM. It gives new insights into the mechanism of recurrent miscarriage. |
| first_indexed | 2024-03-12T06:04:12Z |
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| id | doaj.art-da41b6c3ebb04af9899d675bb5ca6c5d |
| institution | Directory Open Access Journal |
| issn | 2352-3042 |
| language | English |
| last_indexed | 2024-03-12T06:04:12Z |
| publishDate | 2020-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Genes and Diseases |
| spelling | doaj.art-da41b6c3ebb04af9899d675bb5ca6c5d2023-09-03T03:48:11ZengKeAi Communications Co., Ltd.Genes and Diseases2352-30422020-09-0173414423CircRNA expression profiles in decidual tissue of patients with early recurrent miscarriageChunli Li0Xuemei Chen1Xueqing Liu2Xiaoli Liu3Junlin He4Yubin Ding5Chao Tong6Chuan Peng7Yingxiong Wang8Rufei Gao9Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China; Department of Clinical Laboratory, Chongqing Health Center for Women and Children, Chongqing, 401174, China; Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, 400016, ChinaLaboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China; Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, 400016, ChinaLaboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China; Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, 400016, ChinaDepartment of Family Planning, Chongqing Health Center for Women and Children, Chongqing, 401174, PR ChinaLaboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China; Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, 400016, ChinaLaboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China; Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, 400016, ChinaJoint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, 400016, China; Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, ChinaLaboratory of Maternal and Fetal Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, ChinaLaboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China; Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, 400016, China; Corresponding author. Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, No.1 Medical College Road, Chongqing, 400016, China.Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China; Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, 400016, China; Corresponding author. Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, China.Circular RNAs (circRNAs) are a novel class of endogenous noncoding RNAs that play important roles in gene expression regulation. This study aimed to evaluate the potential role of circRNAs in decidual tissue of patients with early recurrent miscarriage (RM). We constructed circRNA expression profiles in decidual tissue using microarray data. A total of 123 differentially expressed circRNAs, including 78 upregulated and 45 downregulated circRNAs were detected in the early RM group compared with the control group (P < 0.05). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis also revealed the enrichment of specific circRNAs. The verified circRNA-targeted miRNA-mRNA network was constructed, most of the circRNAs harbored miRNA binding sites. The network involved 3 circRNAs, 27 microRNAs and 82 mRNAs. Hsa_circRNA_103092- miR-224-PRLR network was selected to verify by qRT-PCR. These results showed that circRNAs are aberrantly expressed in the decidual tissue in early RM patients and play potential roles in the development of early RM. It gives new insights into the mechanism of recurrent miscarriage.http://www.sciencedirect.com/science/article/pii/S235230421930039XCircular RNAsDecidual tissueEarly recurrent miscarriageExpression profilesMicroarray |
| spellingShingle | Chunli Li Xuemei Chen Xueqing Liu Xiaoli Liu Junlin He Yubin Ding Chao Tong Chuan Peng Yingxiong Wang Rufei Gao CircRNA expression profiles in decidual tissue of patients with early recurrent miscarriage Genes and Diseases Circular RNAs Decidual tissue Early recurrent miscarriage Expression profiles Microarray |
| title | CircRNA expression profiles in decidual tissue of patients with early recurrent miscarriage |
| title_full | CircRNA expression profiles in decidual tissue of patients with early recurrent miscarriage |
| title_fullStr | CircRNA expression profiles in decidual tissue of patients with early recurrent miscarriage |
| title_full_unstemmed | CircRNA expression profiles in decidual tissue of patients with early recurrent miscarriage |
| title_short | CircRNA expression profiles in decidual tissue of patients with early recurrent miscarriage |
| title_sort | circrna expression profiles in decidual tissue of patients with early recurrent miscarriage |
| topic | Circular RNAs Decidual tissue Early recurrent miscarriage Expression profiles Microarray |
| url | http://www.sciencedirect.com/science/article/pii/S235230421930039X |
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