Molecular Authentication, Propagation Trials and Field Establishment of Greek Native Genotypes of <i>Sambucus nigra</i> L. (Caprifoliaceae): Setting the Basis for Domestication and Sustainable Utilization
In the context of sustainable utilisation of valuable but neglected plant resources, a multifaceted study is presented herein for Greek native genotypes of elderberry (<i>Sambucus nigra</i> L., Caprifoliaceae), a species with an appreciated pharmaceutical and nutritional value. At the fi...
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MDPI AG
2022-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/1/114 |
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| author | Eleftherios Karapatzak Olga Dichala Ioannis Ganopoulos Antonis Karydas Katerina Papanastasi Dimitris Kyrkas Paraskevi Yfanti Nikos Nikisianis Dimitrios Fotakis Giorgos Patakioutas Eleni Maloupa Nikos Krigas |
| author_facet | Eleftherios Karapatzak Olga Dichala Ioannis Ganopoulos Antonis Karydas Katerina Papanastasi Dimitris Kyrkas Paraskevi Yfanti Nikos Nikisianis Dimitrios Fotakis Giorgos Patakioutas Eleni Maloupa Nikos Krigas |
| author_sort | Eleftherios Karapatzak |
| collection | DOAJ |
| description | In the context of sustainable utilisation of valuable but neglected plant resources, a multifaceted study is presented herein for Greek native genotypes of elderberry (<i>Sambucus nigra</i> L., Caprifoliaceae), a species with an appreciated pharmaceutical and nutritional value. At the first phase, fresh plant materials (leaves, softwood cuttings) were collected from nine geographically separated genotypes originating from wild-growing Greek native germplasm of <i>S. nigra.</i> The leaf samples were genetically authenticated using DNA barcoding (ITS2). The next phase included the development of an asexual propagation protocol via cuttings which included screening of the collected genotypes in terms of propagation success, and further experimentation over a two-year period on a prioritised genotype. The propagation results highlighted the importance of external application of indole-3-butyric acid (IBA) rooting hormone at levels of 2000–4000 ppm, which consistently presented high rooting rates (100%) of summer softwood, leafy cuttings of apical or sub-apical type. At the same time, rooting quality in terms of root number and length as well as early plant growth after rooting, were improved by external hormone application resulting in high rates of plant survival. This study reports first-time data on multifaceted assessment of Greek native <i>S. nigra</i> genotypes on molecular authentication and asexual propagation, thus ultimately setting the basis for domestication and sustainable utilization of this species. |
| first_indexed | 2024-03-10T03:07:01Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T03:07:01Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-da42bba12edd40c3a9daeeabf8c8754f2023-11-23T12:38:30ZengMDPI AGAgronomy2073-43952022-01-0112111410.3390/agronomy12010114Molecular Authentication, Propagation Trials and Field Establishment of Greek Native Genotypes of <i>Sambucus nigra</i> L. (Caprifoliaceae): Setting the Basis for Domestication and Sustainable UtilizationEleftherios Karapatzak0Olga Dichala1Ioannis Ganopoulos2Antonis Karydas3Katerina Papanastasi4Dimitris Kyrkas5Paraskevi Yfanti6Nikos Nikisianis7Dimitrios Fotakis8Giorgos Patakioutas9Eleni Maloupa10Nikos Krigas11Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization-Demeter, P.O. Box 60458, Thermi, 57001 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization-Demeter, P.O. Box 60458, Thermi, 57001 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization-Demeter, P.O. Box 60458, Thermi, 57001 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization-Demeter, P.O. Box 60458, Thermi, 57001 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization-Demeter, P.O. Box 60458, Thermi, 57001 Thessaloniki, GreeceDepartment of Agriculture, University of Ioannina, 47100 Arta, GreeceDepartment of Agriculture, University of Ioannina, 47100 Arta, GreeceSystada General Partnership, Kalamaria, 55133 Thessaloniki, GreeceForest Research Institute, Hellenic Agricultural Organization-Demeter, Vassilika, 57006 Thessaloniki, GreeceDepartment of Agriculture, University of Ioannina, 47100 Arta, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization-Demeter, P.O. Box 60458, Thermi, 57001 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization-Demeter, P.O. Box 60458, Thermi, 57001 Thessaloniki, GreeceIn the context of sustainable utilisation of valuable but neglected plant resources, a multifaceted study is presented herein for Greek native genotypes of elderberry (<i>Sambucus nigra</i> L., Caprifoliaceae), a species with an appreciated pharmaceutical and nutritional value. At the first phase, fresh plant materials (leaves, softwood cuttings) were collected from nine geographically separated genotypes originating from wild-growing Greek native germplasm of <i>S. nigra.</i> The leaf samples were genetically authenticated using DNA barcoding (ITS2). The next phase included the development of an asexual propagation protocol via cuttings which included screening of the collected genotypes in terms of propagation success, and further experimentation over a two-year period on a prioritised genotype. The propagation results highlighted the importance of external application of indole-3-butyric acid (IBA) rooting hormone at levels of 2000–4000 ppm, which consistently presented high rooting rates (100%) of summer softwood, leafy cuttings of apical or sub-apical type. At the same time, rooting quality in terms of root number and length as well as early plant growth after rooting, were improved by external hormone application resulting in high rates of plant survival. This study reports first-time data on multifaceted assessment of Greek native <i>S. nigra</i> genotypes on molecular authentication and asexual propagation, thus ultimately setting the basis for domestication and sustainable utilization of this species.https://www.mdpi.com/2073-4395/12/1/114elderberrysuper fruitsbiodiversityex situ conservationdomesticationDNA barcoding |
| spellingShingle | Eleftherios Karapatzak Olga Dichala Ioannis Ganopoulos Antonis Karydas Katerina Papanastasi Dimitris Kyrkas Paraskevi Yfanti Nikos Nikisianis Dimitrios Fotakis Giorgos Patakioutas Eleni Maloupa Nikos Krigas Molecular Authentication, Propagation Trials and Field Establishment of Greek Native Genotypes of <i>Sambucus nigra</i> L. (Caprifoliaceae): Setting the Basis for Domestication and Sustainable Utilization Agronomy elderberry super fruits biodiversity ex situ conservation domestication DNA barcoding |
| title | Molecular Authentication, Propagation Trials and Field Establishment of Greek Native Genotypes of <i>Sambucus nigra</i> L. (Caprifoliaceae): Setting the Basis for Domestication and Sustainable Utilization |
| title_full | Molecular Authentication, Propagation Trials and Field Establishment of Greek Native Genotypes of <i>Sambucus nigra</i> L. (Caprifoliaceae): Setting the Basis for Domestication and Sustainable Utilization |
| title_fullStr | Molecular Authentication, Propagation Trials and Field Establishment of Greek Native Genotypes of <i>Sambucus nigra</i> L. (Caprifoliaceae): Setting the Basis for Domestication and Sustainable Utilization |
| title_full_unstemmed | Molecular Authentication, Propagation Trials and Field Establishment of Greek Native Genotypes of <i>Sambucus nigra</i> L. (Caprifoliaceae): Setting the Basis for Domestication and Sustainable Utilization |
| title_short | Molecular Authentication, Propagation Trials and Field Establishment of Greek Native Genotypes of <i>Sambucus nigra</i> L. (Caprifoliaceae): Setting the Basis for Domestication and Sustainable Utilization |
| title_sort | molecular authentication propagation trials and field establishment of greek native genotypes of i sambucus nigra i l caprifoliaceae setting the basis for domestication and sustainable utilization |
| topic | elderberry super fruits biodiversity ex situ conservation domestication DNA barcoding |
| url | https://www.mdpi.com/2073-4395/12/1/114 |
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