Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test
BackgroundGabapentin is thought to exert an effect through the voltage-dependent calcium channel. Vitamin E is a widely known antioxidant which neutralizes the harmful effect of ROS which is considered to play a prominent role in various painful conditions. This study was therefore conducted to asse...
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Korean Society of Anesthesiologists
2012-11-01
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| Series: | Korean Journal of Anesthesiology |
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| Online Access: | http://ekja.org/upload/pdf/kjae-63-447.pdf |
| _version_ | 1818605887805194240 |
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| author | Myoung-Joong Kim Won Hyung Lee Young-Kwon Ko Boo Hwi Hong |
| author_facet | Myoung-Joong Kim Won Hyung Lee Young-Kwon Ko Boo Hwi Hong |
| author_sort | Myoung-Joong Kim |
| collection | DOAJ |
| description | BackgroundGabapentin is thought to exert an effect through the voltage-dependent calcium channel. Vitamin E is a widely known antioxidant which neutralizes the harmful effect of ROS which is considered to play a prominent role in various painful conditions. This study was therefore conducted to assess the antinociceptive effects of gabapentin and vitamin E and the interaction of these drugs in the modulation of pain in rats subjected to a formalin test.MethodsSprague-Dawley rats with a lumbar intrathecal catheter were tested for their paw flinches by 5% formalin injection after intrathecal injection of gabapentin or vitamin E. After obtaining dose-response curves for each drug, the effect of the combination was tested by the total dose fraction value and isobolographic analysis.ResultsWhen a single drug was injected intrathecally, significant dose-dependent decreases in flinches were shown only in the late phase. ED50 values of intrathecal gabapentin and vitamin E in the late phase were 75.3 ± 9.58 µg, and 17.56 ± 1.65 mg/kg respectively. The combination of gabapentin and vitamin E produced dose-dependent decreases in the number of flinches in both phases induced by the formalin test. The ED50 value of the combination was lower than the theoretical additive values in the late phase, but did not show a significant difference with the theoretical additive value.ConclusionsGabapentin and vitamin E (by itself) have no antinociceptive effect in the early phase; however their combination has shown an antinociceptive effect. In addition, they show additive effects in the late phase of the formalin test. |
| first_indexed | 2024-12-16T14:02:06Z |
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| id | doaj.art-da435d5a42114f9b9bff448d330db832 |
| institution | Directory Open Access Journal |
| issn | 2005-6419 2005-7563 |
| language | English |
| last_indexed | 2024-12-16T14:02:06Z |
| publishDate | 2012-11-01 |
| publisher | Korean Society of Anesthesiologists |
| record_format | Article |
| series | Korean Journal of Anesthesiology |
| spelling | doaj.art-da435d5a42114f9b9bff448d330db8322022-12-21T22:29:04ZengKorean Society of AnesthesiologistsKorean Journal of Anesthesiology2005-64192005-75632012-11-0163544745310.4097/kjae.2012.63.5.4477456Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin testMyoung-Joong Kim0Won Hyung Lee1Young-Kwon Ko2Boo Hwi Hong3Department of Anesthesiology and Pain Medicine, Chungnam National University School of Medicine, Daejeon, Korea.Department of Anesthesiology and Pain Medicine, Chungnam National University School of Medicine, Daejeon, Korea.Department of Anesthesiology and Pain Medicine, Chungnam National University School of Medicine, Daejeon, Korea.Department of Anesthesiology and Pain Medicine, Chungnam National University School of Medicine, Daejeon, Korea.BackgroundGabapentin is thought to exert an effect through the voltage-dependent calcium channel. Vitamin E is a widely known antioxidant which neutralizes the harmful effect of ROS which is considered to play a prominent role in various painful conditions. This study was therefore conducted to assess the antinociceptive effects of gabapentin and vitamin E and the interaction of these drugs in the modulation of pain in rats subjected to a formalin test.MethodsSprague-Dawley rats with a lumbar intrathecal catheter were tested for their paw flinches by 5% formalin injection after intrathecal injection of gabapentin or vitamin E. After obtaining dose-response curves for each drug, the effect of the combination was tested by the total dose fraction value and isobolographic analysis.ResultsWhen a single drug was injected intrathecally, significant dose-dependent decreases in flinches were shown only in the late phase. ED50 values of intrathecal gabapentin and vitamin E in the late phase were 75.3 ± 9.58 µg, and 17.56 ± 1.65 mg/kg respectively. The combination of gabapentin and vitamin E produced dose-dependent decreases in the number of flinches in both phases induced by the formalin test. The ED50 value of the combination was lower than the theoretical additive values in the late phase, but did not show a significant difference with the theoretical additive value.ConclusionsGabapentin and vitamin E (by itself) have no antinociceptive effect in the early phase; however their combination has shown an antinociceptive effect. In addition, they show additive effects in the late phase of the formalin test.http://ekja.org/upload/pdf/kjae-63-447.pdfantinociceptive effectsgabapentininteractionintrathecalvitamin e |
| spellingShingle | Myoung-Joong Kim Won Hyung Lee Young-Kwon Ko Boo Hwi Hong Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test Korean Journal of Anesthesiology antinociceptive effects gabapentin interaction intrathecal vitamin e |
| title | Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test |
| title_full | Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test |
| title_fullStr | Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test |
| title_full_unstemmed | Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test |
| title_short | Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test |
| title_sort | antinociceptive drug interaction between intrathecal vitamin e and gabapentin in the rat formalin test |
| topic | antinociceptive effects gabapentin interaction intrathecal vitamin e |
| url | http://ekja.org/upload/pdf/kjae-63-447.pdf |
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