Human Antibody Repertoire following Ebola Virus Infection and Vaccination
Summary: Limited knowledge exists on the quality of polyclonal antibody response generated following Ebola virus (EBOV) infection compared with vaccination. Polyclonal antibody repertoire in plasma following EBOV infection in survivors was compared with ChAd3-MVA prime-boost human vaccination. Highe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-03-01
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| Series: | iScience |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004220301048 |
| _version_ | 1828760943725641728 |
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| author | Sandra Fuentes Supriya Ravichandran Elizabeth M. Coyle Laura Klenow Surender Khurana |
| author_facet | Sandra Fuentes Supriya Ravichandran Elizabeth M. Coyle Laura Klenow Surender Khurana |
| author_sort | Sandra Fuentes |
| collection | DOAJ |
| description | Summary: Limited knowledge exists on the quality of polyclonal antibody response generated following Ebola virus (EBOV) infection compared with vaccination. Polyclonal antibody repertoire in plasma following EBOV infection in survivors was compared with ChAd3-MVA prime-boost human vaccination. Higher antibody binding and affinity to GP was observed in survivors compared with vaccinated plasma that correlated with EBOV neutralization. Surprisingly, a predominant IgM response was generated after prime-boost vaccination, whereas survivors demonstrated IgG-dominant antibody response. EBOV infection induced more diverse antibody epitope repertoire compared with vaccination. A strong binding to antigenic sites in the fusion peptide and another in the highly conserved GP2-HR2 domain was preferentially recognized by EBOV survivors than vaccinated individuals that correlated strongly with EBOV neutralization titers. These findings will help development and evaluation of effective Ebola countermeasures including therapeutics and vaccines. : Virology; Viral Microbiology Subject Areas: Virology, Viral Microbiology |
| first_indexed | 2024-12-11T01:22:24Z |
| format | Article |
| id | doaj.art-da4edc9e21a942c1a093360a5cfb59ec |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-11T01:22:24Z |
| publishDate | 2020-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-da4edc9e21a942c1a093360a5cfb59ec2022-12-22T01:25:40ZengElsevieriScience2589-00422020-03-01233Human Antibody Repertoire following Ebola Virus Infection and VaccinationSandra Fuentes0Supriya Ravichandran1Elizabeth M. Coyle2Laura Klenow3Surender Khurana4Division of Viral Products, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), 10903 New Hampshire Avenue, Silver Spring, MD 20993, USADivision of Viral Products, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), 10903 New Hampshire Avenue, Silver Spring, MD 20993, USADivision of Viral Products, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), 10903 New Hampshire Avenue, Silver Spring, MD 20993, USADivision of Viral Products, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), 10903 New Hampshire Avenue, Silver Spring, MD 20993, USADivision of Viral Products, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), 10903 New Hampshire Avenue, Silver Spring, MD 20993, USA; Corresponding authorSummary: Limited knowledge exists on the quality of polyclonal antibody response generated following Ebola virus (EBOV) infection compared with vaccination. Polyclonal antibody repertoire in plasma following EBOV infection in survivors was compared with ChAd3-MVA prime-boost human vaccination. Higher antibody binding and affinity to GP was observed in survivors compared with vaccinated plasma that correlated with EBOV neutralization. Surprisingly, a predominant IgM response was generated after prime-boost vaccination, whereas survivors demonstrated IgG-dominant antibody response. EBOV infection induced more diverse antibody epitope repertoire compared with vaccination. A strong binding to antigenic sites in the fusion peptide and another in the highly conserved GP2-HR2 domain was preferentially recognized by EBOV survivors than vaccinated individuals that correlated strongly with EBOV neutralization titers. These findings will help development and evaluation of effective Ebola countermeasures including therapeutics and vaccines. : Virology; Viral Microbiology Subject Areas: Virology, Viral Microbiologyhttp://www.sciencedirect.com/science/article/pii/S2589004220301048 |
| spellingShingle | Sandra Fuentes Supriya Ravichandran Elizabeth M. Coyle Laura Klenow Surender Khurana Human Antibody Repertoire following Ebola Virus Infection and Vaccination iScience |
| title | Human Antibody Repertoire following Ebola Virus Infection and Vaccination |
| title_full | Human Antibody Repertoire following Ebola Virus Infection and Vaccination |
| title_fullStr | Human Antibody Repertoire following Ebola Virus Infection and Vaccination |
| title_full_unstemmed | Human Antibody Repertoire following Ebola Virus Infection and Vaccination |
| title_short | Human Antibody Repertoire following Ebola Virus Infection and Vaccination |
| title_sort | human antibody repertoire following ebola virus infection and vaccination |
| url | http://www.sciencedirect.com/science/article/pii/S2589004220301048 |
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