Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons
Abstract The Golgi apparatus is the major sorting hub in the secretory pathway and particularly important for protein sorting in neurons. Knowledge about protein localization in Golgi compartments is largely based on work in cell lines. Here, we systematically compared protein localization of 21 end...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-29998-8 |
| _version_ | 1797864936046592000 |
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| author | Danique M. van Bommel Ruud F. Toonen Matthijs Verhage |
| author_facet | Danique M. van Bommel Ruud F. Toonen Matthijs Verhage |
| author_sort | Danique M. van Bommel |
| collection | DOAJ |
| description | Abstract The Golgi apparatus is the major sorting hub in the secretory pathway and particularly important for protein sorting in neurons. Knowledge about protein localization in Golgi compartments is largely based on work in cell lines. Here, we systematically compared protein localization of 21 endogenous proteins in the Golgi apparatus of mouse neurons using confocal microscopy and line scan analysis. We localized these proteins by measuring the distance relative to the canonical TGN marker TGN38. Based on this, proteins fell into three groups: upstream of, overlapping with or downstream of TGN38. Seven proteins showed complete overlap with TGN38, while proteins downstream of TGN38 were located at varying distances from TGN38. Proteins upstream of TGN38 were localized in between TGN38 and the cis-/medial Golgi markers Giantin and GM130. This localization was consistent with protein function. Our data provide an overview of the relative localization of endogenous proteins in the Golgi of primary mouse neurons. |
| first_indexed | 2024-04-09T22:59:58Z |
| format | Article |
| id | doaj.art-da4f431dcb64471086597fedb9d67fe1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:59:58Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-da4f431dcb64471086597fedb9d67fe12023-03-22T11:02:52ZengNature PortfolioScientific Reports2045-23222023-02-011311910.1038/s41598-023-29998-8Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neuronsDanique M. van Bommel0Ruud F. Toonen1Matthijs Verhage2Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) AmsterdamDepartment of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) AmsterdamDepartment of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) AmsterdamAbstract The Golgi apparatus is the major sorting hub in the secretory pathway and particularly important for protein sorting in neurons. Knowledge about protein localization in Golgi compartments is largely based on work in cell lines. Here, we systematically compared protein localization of 21 endogenous proteins in the Golgi apparatus of mouse neurons using confocal microscopy and line scan analysis. We localized these proteins by measuring the distance relative to the canonical TGN marker TGN38. Based on this, proteins fell into three groups: upstream of, overlapping with or downstream of TGN38. Seven proteins showed complete overlap with TGN38, while proteins downstream of TGN38 were located at varying distances from TGN38. Proteins upstream of TGN38 were localized in between TGN38 and the cis-/medial Golgi markers Giantin and GM130. This localization was consistent with protein function. Our data provide an overview of the relative localization of endogenous proteins in the Golgi of primary mouse neurons.https://doi.org/10.1038/s41598-023-29998-8 |
| spellingShingle | Danique M. van Bommel Ruud F. Toonen Matthijs Verhage Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons Scientific Reports |
| title | Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons |
| title_full | Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons |
| title_fullStr | Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons |
| title_full_unstemmed | Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons |
| title_short | Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons |
| title_sort | mapping localization of 21 endogenous proteins in the golgi apparatus of rodent neurons |
| url | https://doi.org/10.1038/s41598-023-29998-8 |
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