Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk
Flunixin meglumine (FM) is a novel nonsteroidal anti-inflammatory drug for animals, which has antipyretic, analgesic, and anti-inflammatory effects. The drug, which was originally used to relieve inflammation in horses, musculoskeletal disorders, and pain, has been approved for use in endotoxemia, i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
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| Online Access: | http://dx.doi.org/10.1080/09540105.2017.1364710 |
| _version_ | 1818394684407414784 |
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| author | Lu Lin Wei Jiang Liguang Xu Liqiang Liu Shanshan Song Hua Kuang |
| author_facet | Lu Lin Wei Jiang Liguang Xu Liqiang Liu Shanshan Song Hua Kuang |
| author_sort | Lu Lin |
| collection | DOAJ |
| description | Flunixin meglumine (FM) is a novel nonsteroidal anti-inflammatory drug for animals, which has antipyretic, analgesic, and anti-inflammatory effects. The drug, which was originally used to relieve inflammation in horses, musculoskeletal disorders, and pain, has been approved for use in endotoxemia, infectious diseases in swine, etc. A sensitive anti-FM monoclonal antibody 2H4 was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay and immunochromatographic strip assay for the detection of FM in milk. The complete antigen and coating antigen were conjugated with bovine serum albumin and ovalbumin, respectively. The monoclonal antibody 2H4, with a half inhibition concentration of 0.29 ng/mL, had a limit of detection of 0.432 ng/mL and a linear range of detection of 0.08664–0.97226 ng/mL. A sensitive and convenient immunochromatographic strip assay was developed with an FM cutoff value of 0.29 ng/mL. The developed methods were suitable for the detection of FM in milk. |
| first_indexed | 2024-12-14T06:05:07Z |
| format | Article |
| id | doaj.art-da5229dac09244b2a524f1c45f10dc0d |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-14T06:05:07Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-da5229dac09244b2a524f1c45f10dc0d2022-12-21T23:14:20ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-0129119320310.1080/09540105.2017.13647101364710Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milkLu Lin0Wei Jiang1Liguang Xu2Liqiang Liu3Shanshan Song4Hua Kuang5State Key Lab of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, Jiangnan UniversityFlunixin meglumine (FM) is a novel nonsteroidal anti-inflammatory drug for animals, which has antipyretic, analgesic, and anti-inflammatory effects. The drug, which was originally used to relieve inflammation in horses, musculoskeletal disorders, and pain, has been approved for use in endotoxemia, infectious diseases in swine, etc. A sensitive anti-FM monoclonal antibody 2H4 was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay and immunochromatographic strip assay for the detection of FM in milk. The complete antigen and coating antigen were conjugated with bovine serum albumin and ovalbumin, respectively. The monoclonal antibody 2H4, with a half inhibition concentration of 0.29 ng/mL, had a limit of detection of 0.432 ng/mL and a linear range of detection of 0.08664–0.97226 ng/mL. A sensitive and convenient immunochromatographic strip assay was developed with an FM cutoff value of 0.29 ng/mL. The developed methods were suitable for the detection of FM in milk.http://dx.doi.org/10.1080/09540105.2017.1364710flunixin megluminemonoclonal antibodyimmunochromatographic strip assayic-elisamilk |
| spellingShingle | Lu Lin Wei Jiang Liguang Xu Liqiang Liu Shanshan Song Hua Kuang Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk Food and Agricultural Immunology flunixin meglumine monoclonal antibody immunochromatographic strip assay ic-elisa milk |
| title | Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk |
| title_full | Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk |
| title_fullStr | Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk |
| title_full_unstemmed | Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk |
| title_short | Development of IC-ELISA and immunochromatographic strip assay for the detection of flunixin meglumine in milk |
| title_sort | development of ic elisa and immunochromatographic strip assay for the detection of flunixin meglumine in milk |
| topic | flunixin meglumine monoclonal antibody immunochromatographic strip assay ic-elisa milk |
| url | http://dx.doi.org/10.1080/09540105.2017.1364710 |
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