Co-existence of OXA-48 and NDM-1 in colistin resistant Pseudomonas aeruginosa ST235
ABSTRACTHere, we presented 11 cases with colistin-resistant Pseudomonas aeruginosa infection and co-existence of OXA-48 and NDM-1 in the ST235 high-risk clone. The molecular analyses were performed by Sanger sequencing and RT–PCR. The eight patients (72.7%) had an invasive infection and three (27.3%...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1713025 |
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| author | Cansel Vatansever Sirin Menekse Ozlem Dogan Lal Sude Gucer Berna Ozer Onder Ergonul Fusun Can |
| author_facet | Cansel Vatansever Sirin Menekse Ozlem Dogan Lal Sude Gucer Berna Ozer Onder Ergonul Fusun Can |
| author_sort | Cansel Vatansever |
| collection | DOAJ |
| description | ABSTRACTHere, we presented 11 cases with colistin-resistant Pseudomonas aeruginosa infection and co-existence of OXA-48 and NDM-1 in the ST235 high-risk clone. The molecular analyses were performed by Sanger sequencing and RT–PCR. The eight patients (72.7%) had an invasive infection and three (27.3%) had colonization. The 30-day mortality rate was 87.5% (7/8). Three patients (37.5%, 3/8) received colistin therapy before isolation of P. aeruginosa. In the Multilocus sequence typing (MLST) analysis of 11 isolates, eight (72.7%) isolates belonged to P. aeruginosa ST235 clone. All isolates were NDM-1 positive, and nine isolates (81.8%) were found to be positive for both OXA-48 and NDM-1. Sequences of pmrAB and phoPQ revealed numerous insertions and deletions in all isolates. In 10 isolates pmrAB and phoPQ were found to be upregulated. In conclusion, the co-existence of OXA-48 and NDM-1 genes in colistin-resistant P. aeruginosa ST235 high-risk clone indicates the spread of carbapenemases in clinical isolates and highlights need of continuous surveillance for high-risk clones of P. aeruginosa. |
| first_indexed | 2024-03-07T17:24:32Z |
| format | Article |
| id | doaj.art-da53e837514841859f0382ddcdfa8915 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-04-25T00:52:11Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-da53e837514841859f0382ddcdfa89152024-03-11T16:04:24ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-019115215410.1080/22221751.2020.1713025Co-existence of OXA-48 and NDM-1 in colistin resistant Pseudomonas aeruginosa ST235Cansel Vatansever0Sirin Menekse1Ozlem Dogan2Lal Sude Gucer3Berna Ozer4Onder Ergonul5Fusun Can6Department of Infectious Diseases and Clinical Microbiology, Koc University School of Medicine, Istanbul, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Kosuyolu State Hospital, Istanbul, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Koc University School of Medicine, Istanbul, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Koc University School of Medicine, Istanbul, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Koc University School of Medicine, Istanbul, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Koc University School of Medicine, Istanbul, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Koc University School of Medicine, Istanbul, TurkeyABSTRACTHere, we presented 11 cases with colistin-resistant Pseudomonas aeruginosa infection and co-existence of OXA-48 and NDM-1 in the ST235 high-risk clone. The molecular analyses were performed by Sanger sequencing and RT–PCR. The eight patients (72.7%) had an invasive infection and three (27.3%) had colonization. The 30-day mortality rate was 87.5% (7/8). Three patients (37.5%, 3/8) received colistin therapy before isolation of P. aeruginosa. In the Multilocus sequence typing (MLST) analysis of 11 isolates, eight (72.7%) isolates belonged to P. aeruginosa ST235 clone. All isolates were NDM-1 positive, and nine isolates (81.8%) were found to be positive for both OXA-48 and NDM-1. Sequences of pmrAB and phoPQ revealed numerous insertions and deletions in all isolates. In 10 isolates pmrAB and phoPQ were found to be upregulated. In conclusion, the co-existence of OXA-48 and NDM-1 genes in colistin-resistant P. aeruginosa ST235 high-risk clone indicates the spread of carbapenemases in clinical isolates and highlights need of continuous surveillance for high-risk clones of P. aeruginosa.https://www.tandfonline.com/doi/10.1080/22221751.2020.1713025P. aeruginosacolistin resistancehigh-risk cloneST235OXA-48NDM-1 |
| spellingShingle | Cansel Vatansever Sirin Menekse Ozlem Dogan Lal Sude Gucer Berna Ozer Onder Ergonul Fusun Can Co-existence of OXA-48 and NDM-1 in colistin resistant Pseudomonas aeruginosa ST235 Emerging Microbes and Infections P. aeruginosa colistin resistance high-risk clone ST235 OXA-48 NDM-1 |
| title | Co-existence of OXA-48 and NDM-1 in colistin resistant Pseudomonas aeruginosa ST235 |
| title_full | Co-existence of OXA-48 and NDM-1 in colistin resistant Pseudomonas aeruginosa ST235 |
| title_fullStr | Co-existence of OXA-48 and NDM-1 in colistin resistant Pseudomonas aeruginosa ST235 |
| title_full_unstemmed | Co-existence of OXA-48 and NDM-1 in colistin resistant Pseudomonas aeruginosa ST235 |
| title_short | Co-existence of OXA-48 and NDM-1 in colistin resistant Pseudomonas aeruginosa ST235 |
| title_sort | co existence of oxa 48 and ndm 1 in colistin resistant pseudomonas aeruginosa st235 |
| topic | P. aeruginosa colistin resistance high-risk clone ST235 OXA-48 NDM-1 |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1713025 |
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