Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of <i>Klebsiella pneumoniae</i>
The emergence of hyper-virulent and multidrug-resistant (MDR) strains of <i>Klebsiella pneumoniae</i> isolated from patients with hospital- and community-acquired infections is a serious health problem that increases mortality. The molecular analysis of virulome expression related to ant...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/19/14754 |
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| author | Gloria Luz Paniagua-Contreras Areli Bautista-Cerón Rosario Morales-Espinosa Gabriela Delgado Felipe Vaca-Paniagua Clara Estela Díaz-Velásquez Aldo Hugo de la Cruz-Montoya Luis Rey García-Cortés María Patricia Sánchez-Yáñez Eric Monroy-Pérez |
| author_facet | Gloria Luz Paniagua-Contreras Areli Bautista-Cerón Rosario Morales-Espinosa Gabriela Delgado Felipe Vaca-Paniagua Clara Estela Díaz-Velásquez Aldo Hugo de la Cruz-Montoya Luis Rey García-Cortés María Patricia Sánchez-Yáñez Eric Monroy-Pérez |
| author_sort | Gloria Luz Paniagua-Contreras |
| collection | DOAJ |
| description | The emergence of hyper-virulent and multidrug-resistant (MDR) strains of <i>Klebsiella pneumoniae</i> isolated from patients with hospital- and community-acquired infections is a serious health problem that increases mortality. The molecular analysis of virulome expression related to antimicrobial-resistant genotype and infection type in <i>K. pneumoniae</i> strains isolated from patients with hospital- and community-acquired infections has been poorly studied. In this study, we analyzed the overall expression of the virulence genotype associated with the antimicrobial resistance genotype and pulse field gel electrophoresis (PFGE) type (PFtype) in <i>K. pneumoniae</i>. We studied 25 strains of <i>K. pneumoniae</i> isolated from patients who developed bacteremia and pneumonia during their hospital stay and 125 strains from outpatients who acquired community-acquired infections. Susceptibility to 12 antimicrobials was determined by Kirby–Bauer. The identification of <i>K. pneumoniae</i> and antibiotic-resistance genes was performed using polymerase chain reaction (PCR). To promote the expression of the virulence genes of <i>K. pneumoniae</i>, an in vitro infection model was used in human epithelial cell lines A549 and A431. Bacterial RNA was extracted with the QIAcube robotic workstation, and reverse transcription to cDNA was performed with the Reverse Transcription QuantiTect kit (Qiagen). The determination of the expression of the virulence genes was performed by real-time PCR. In addition, 57.3% (n = 86) of the strains isolated from patients with hospital- and community-acquired infections were multidrug-resistant (MDR), mainly to beta-lactam antibiotics (CB, AM, CFX, and CF), aminoglycosides (GE), quinolones (CPF and NOF), nitrofurantoin (NF), and sulfamethoxazole/trimethoprim (SXT). The most frequently expressed genes among strains isolated from hospital- and community-acquired infections were adhesion-type, <i>ycfm</i> (80%), <i>mrkD</i> (51.3%), and <i>fimH</i> (30.7%); iron uptake, <i>irp2</i> (84%)<i>, fyuA</i> (68.7%), <i>entB</i> (64.7%), and <i>irp1</i> (56.7%); and protectins, <i>rpmA</i> (26%), which were related to antibiotic-resistance genes, <i>bla<sub>TEM</sub></i> (96%), <i>bla<sub>SHV</sub></i> (64%), <i>bla<sub>CITM</sub></i> (52.6%), <i>bla<sub>CTXM-1</sub></i> (44.7%), <i>tetA</i> (74%), <i>sul1</i> (57.3%), <i>aac(3)-IV</i> (40.7%), and <i>aadA1</i> (36%). The results showed the existence of different patterns of expression of virulome related to the genotype of resistance to antimicrobials and to the PFtypes in the strains of <i>K. pneumoniae</i> that cause hospital- and community-acquired infections. These findings are important and may contribute to improving medical treatment strategies against infections caused by <i>K. pneumoniae</i>. |
| first_indexed | 2024-03-09T13:53:29Z |
| format | Article |
| id | doaj.art-da54d38e4ff54ea6b16e706f17a01674 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T13:53:29Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-da54d38e4ff54ea6b16e706f17a016742023-11-30T20:46:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124191475410.3390/ijms241914754Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of <i>Klebsiella pneumoniae</i>Gloria Luz Paniagua-Contreras0Areli Bautista-Cerón1Rosario Morales-Espinosa2Gabriela Delgado3Felipe Vaca-Paniagua4Clara Estela Díaz-Velásquez5Aldo Hugo de la Cruz-Montoya6Luis Rey García-Cortés7María Patricia Sánchez-Yáñez8Eric Monroy-Pérez9Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, MexicoFacultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoUnidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, MexicoUnidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, MexicoUnidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, MexicoInstituto Mexicano del Seguro Social, Naucalpan de Juárez 53370, MexicoFacultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, MexicoFacultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, MexicoThe emergence of hyper-virulent and multidrug-resistant (MDR) strains of <i>Klebsiella pneumoniae</i> isolated from patients with hospital- and community-acquired infections is a serious health problem that increases mortality. The molecular analysis of virulome expression related to antimicrobial-resistant genotype and infection type in <i>K. pneumoniae</i> strains isolated from patients with hospital- and community-acquired infections has been poorly studied. In this study, we analyzed the overall expression of the virulence genotype associated with the antimicrobial resistance genotype and pulse field gel electrophoresis (PFGE) type (PFtype) in <i>K. pneumoniae</i>. We studied 25 strains of <i>K. pneumoniae</i> isolated from patients who developed bacteremia and pneumonia during their hospital stay and 125 strains from outpatients who acquired community-acquired infections. Susceptibility to 12 antimicrobials was determined by Kirby–Bauer. The identification of <i>K. pneumoniae</i> and antibiotic-resistance genes was performed using polymerase chain reaction (PCR). To promote the expression of the virulence genes of <i>K. pneumoniae</i>, an in vitro infection model was used in human epithelial cell lines A549 and A431. Bacterial RNA was extracted with the QIAcube robotic workstation, and reverse transcription to cDNA was performed with the Reverse Transcription QuantiTect kit (Qiagen). The determination of the expression of the virulence genes was performed by real-time PCR. In addition, 57.3% (n = 86) of the strains isolated from patients with hospital- and community-acquired infections were multidrug-resistant (MDR), mainly to beta-lactam antibiotics (CB, AM, CFX, and CF), aminoglycosides (GE), quinolones (CPF and NOF), nitrofurantoin (NF), and sulfamethoxazole/trimethoprim (SXT). The most frequently expressed genes among strains isolated from hospital- and community-acquired infections were adhesion-type, <i>ycfm</i> (80%), <i>mrkD</i> (51.3%), and <i>fimH</i> (30.7%); iron uptake, <i>irp2</i> (84%)<i>, fyuA</i> (68.7%), <i>entB</i> (64.7%), and <i>irp1</i> (56.7%); and protectins, <i>rpmA</i> (26%), which were related to antibiotic-resistance genes, <i>bla<sub>TEM</sub></i> (96%), <i>bla<sub>SHV</sub></i> (64%), <i>bla<sub>CITM</sub></i> (52.6%), <i>bla<sub>CTXM-1</sub></i> (44.7%), <i>tetA</i> (74%), <i>sul1</i> (57.3%), <i>aac(3)-IV</i> (40.7%), and <i>aadA1</i> (36%). The results showed the existence of different patterns of expression of virulome related to the genotype of resistance to antimicrobials and to the PFtypes in the strains of <i>K. pneumoniae</i> that cause hospital- and community-acquired infections. These findings are important and may contribute to improving medical treatment strategies against infections caused by <i>K. pneumoniae</i>.https://www.mdpi.com/1422-0067/24/19/14754multidrug-resistantvirulome expressionantimicrobial resistance genotypepulse field gel electrophoresis |
| spellingShingle | Gloria Luz Paniagua-Contreras Areli Bautista-Cerón Rosario Morales-Espinosa Gabriela Delgado Felipe Vaca-Paniagua Clara Estela Díaz-Velásquez Aldo Hugo de la Cruz-Montoya Luis Rey García-Cortés María Patricia Sánchez-Yáñez Eric Monroy-Pérez Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of <i>Klebsiella pneumoniae</i> International Journal of Molecular Sciences multidrug-resistant virulome expression antimicrobial resistance genotype pulse field gel electrophoresis |
| title | Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of <i>Klebsiella pneumoniae</i> |
| title_full | Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of <i>Klebsiella pneumoniae</i> |
| title_fullStr | Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of <i>Klebsiella pneumoniae</i> |
| title_full_unstemmed | Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of <i>Klebsiella pneumoniae</i> |
| title_short | Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of <i>Klebsiella pneumoniae</i> |
| title_sort | extensive expression of the virulome related to antibiotic genotyping in nosocomial strains of i klebsiella pneumoniae i |
| topic | multidrug-resistant virulome expression antimicrobial resistance genotype pulse field gel electrophoresis |
| url | https://www.mdpi.com/1422-0067/24/19/14754 |
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