Expression of Sex-Related Genes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol
Sex emerges out of a delicate dance between a variety of promale, anti-male, and possibly profemale genes. To investigate the role that sex-related genes play in sex determination and gonadal differentiation of fowl, we constructed a male-to-female sex-reversal model of chick induced by diethylstilb...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-01-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311913602130 |
| _version_ | 1819000492898910208 |
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| author | Li-xiu FANG Rui XIN Yi CHE Shi-qing XU |
| author_facet | Li-xiu FANG Rui XIN Yi CHE Shi-qing XU |
| author_sort | Li-xiu FANG |
| collection | DOAJ |
| description | Sex emerges out of a delicate dance between a variety of promale, anti-male, and possibly profemale genes. To investigate the role that sex-related genes play in sex determination and gonadal differentiation of fowl, we constructed a male-to-female sex-reversal model of chick induced by diethylstilbestrol (DES) at onset of incubation (E0). The results of semiquantitative PCR showed that the expression of Sf1, the orphan nuclear receptor steroidogenic factor-1 gene, was put forward from E7d to E5d and up-regulated during E5-7d; the Dmrt1, the double sex and the Mab-3 related to transcription factor 1 gene, was down-regulated during E3-7d. Meanwhile, anti-Müllerian hormone gene (Amh) expressed at a similar level in the genetic females and sex-reversal females before E7d, while no expression products of the three female-specific genes Wpkci, Fet1 and Foxl2 were detected in male-to-female embryos. These findings suggest that the expression of some certain sex-related genes, induced by the exogenous estrogen during period of sex determination and gonadal differentiation, results in the male-to-female sex reversal. Moreover, high activity of Sf1 gene during E5-7d might be related to the profemale process, while low activity of Dmrt1 gene during E3-5d might be anti-male. The expression activity of Amh gene might only contribute to the promale process after E7d, however, it is possibly not an anti-female gene in chick embryos. |
| first_indexed | 2024-12-20T22:34:11Z |
| format | Article |
| id | doaj.art-da55d51e5c714e2bac758564361596ed |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-20T22:34:11Z |
| publishDate | 2013-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-da55d51e5c714e2bac758564361596ed2022-12-21T19:24:40ZengElsevierJournal of Integrative Agriculture2095-31192013-01-01121127135Expression of Sex-Related Genes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to DiethylstilbestrolLi-xiu FANG0Rui XIN1Yi CHE2Shi-qing XU3Department of Applied Biology, Medical College, Soochow University, Suzhou 215123, P.R. ChinaDepartment of Applied Biology, Medical College, Soochow University, Suzhou 215123, P.R. ChinaDepartment of Applied Biology, Medical College, Soochow University, Suzhou 215123, P.R. ChinaCorrespondence XU Shi-qing, Tel: +86-512-65880185, Fax: +86-512-65880186; Department of Applied Biology, Medical College, Soochow University, Suzhou 215123, P.R. ChinaSex emerges out of a delicate dance between a variety of promale, anti-male, and possibly profemale genes. To investigate the role that sex-related genes play in sex determination and gonadal differentiation of fowl, we constructed a male-to-female sex-reversal model of chick induced by diethylstilbestrol (DES) at onset of incubation (E0). The results of semiquantitative PCR showed that the expression of Sf1, the orphan nuclear receptor steroidogenic factor-1 gene, was put forward from E7d to E5d and up-regulated during E5-7d; the Dmrt1, the double sex and the Mab-3 related to transcription factor 1 gene, was down-regulated during E3-7d. Meanwhile, anti-Müllerian hormone gene (Amh) expressed at a similar level in the genetic females and sex-reversal females before E7d, while no expression products of the three female-specific genes Wpkci, Fet1 and Foxl2 were detected in male-to-female embryos. These findings suggest that the expression of some certain sex-related genes, induced by the exogenous estrogen during period of sex determination and gonadal differentiation, results in the male-to-female sex reversal. Moreover, high activity of Sf1 gene during E5-7d might be related to the profemale process, while low activity of Dmrt1 gene during E3-5d might be anti-male. The expression activity of Amh gene might only contribute to the promale process after E7d, however, it is possibly not an anti-female gene in chick embryos.http://www.sciencedirect.com/science/article/pii/S2095311913602130sex reversaldiethylstilbestrolchickensex determinationsexual differentiationsex-related gene |
| spellingShingle | Li-xiu FANG Rui XIN Yi CHE Shi-qing XU Expression of Sex-Related Genes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol Journal of Integrative Agriculture sex reversal diethylstilbestrol chicken sex determination sexual differentiation sex-related gene |
| title | Expression of Sex-Related Genes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol |
| title_full | Expression of Sex-Related Genes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol |
| title_fullStr | Expression of Sex-Related Genes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol |
| title_full_unstemmed | Expression of Sex-Related Genes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol |
| title_short | Expression of Sex-Related Genes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol |
| title_sort | expression of sex related genes in chicken embryos during male to female sex reversal exposure to diethylstilbestrol |
| topic | sex reversal diethylstilbestrol chicken sex determination sexual differentiation sex-related gene |
| url | http://www.sciencedirect.com/science/article/pii/S2095311913602130 |
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