The Mechanisms of BDNF Promoting the Proliferation of Porcine Follicular Granulosa Cells: Role of miR-127 and Involvement of the MAPK-ERK1/2 Pathway
As a member of the neurotrophic family, brain-derived neurotrophic factor (BDNF) provides a key link in the physiological process of mammalian ovarian follicle development, in addition to its functions in the nervous system. The emphasis of this study lay in the impact of BDNF on the proliferation o...
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MDPI AG
2023-03-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/6/1115 |
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| author | Xue Zheng Lu Chen Tong Chen Maosheng Cao Boqi Zhang Chenfeng Yuan Zijiao Zhao Chunjin Li Xu Zhou |
| author_facet | Xue Zheng Lu Chen Tong Chen Maosheng Cao Boqi Zhang Chenfeng Yuan Zijiao Zhao Chunjin Li Xu Zhou |
| author_sort | Xue Zheng |
| collection | DOAJ |
| description | As a member of the neurotrophic family, brain-derived neurotrophic factor (BDNF) provides a key link in the physiological process of mammalian ovarian follicle development, in addition to its functions in the nervous system. The emphasis of this study lay in the impact of BDNF on the proliferation of porcine follicular granulosa cells (GCs) in vitro. BDNF and tyrosine kinase B (TrkB, receptor of BDNF) were detected in porcine follicular GCs. Additionally, cell viability significantly increased during the culture of porcine GCs with BDNF (100 ng/mL) in vitro. However, BDNF knockdown in GCs decreased cell viability and S-phase cells proportion—and BDNF simultaneously regulated the expression of genes linked with cell proliferation (CCND1, p21 and Bcl2) and apoptosis (Bax). Then, the results of the receptor blocking experiment showed that BDNF promoted GC proliferation via TrkB. The high-throughput sequencing showed that BDNF also regulated the expression profiles of miRNAs in GCs. The differential expression profiles were obtained by miRNA sequencing after BDNF (100 ng/mL) treatment with GCs. The sequencing results showed that, after BDNF treatment, 72 significant differentially-expressed miRNAs were detected—5 of which were related to cell process and proliferation signaling pathways confirmed by RT-PCR. Furthermore, studies showed that BDNF promoted GCs’ proliferation by increasing the expression of CCND1, downregulating miR-127 and activating the ERK1/2 signal pathway. Moreover, BDNF indirectly activated the ERK1/2 signal pathway by downregulating miR-127. In conclusion, BDNF promoted porcine GC proliferation by increasing CCND1 expression, downregulating miR-127 and stimulating the MAPK-ERK1/2 signaling cascade. |
| first_indexed | 2024-03-11T07:02:00Z |
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| last_indexed | 2024-03-11T07:02:00Z |
| publishDate | 2023-03-01 |
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| series | Animals |
| spelling | doaj.art-da57c633b0444cd9bd8b4fde6106042d2023-11-17T09:12:36ZengMDPI AGAnimals2076-26152023-03-01136111510.3390/ani13061115The Mechanisms of BDNF Promoting the Proliferation of Porcine Follicular Granulosa Cells: Role of miR-127 and Involvement of the MAPK-ERK1/2 PathwayXue Zheng0Lu Chen1Tong Chen2Maosheng Cao3Boqi Zhang4Chenfeng Yuan5Zijiao Zhao6Chunjin Li7Xu Zhou8Laboratory for Regulation of Reproduction, College of Animal Sciences, Jilin University, Changchun 130062, ChinaLaboratory for Regulation of Reproduction, College of Animal Sciences, Jilin University, Changchun 130062, ChinaLaboratory for Regulation of Reproduction, College of Animal Sciences, Jilin University, Changchun 130062, ChinaLaboratory for Regulation of Reproduction, College of Animal Sciences, Jilin University, Changchun 130062, ChinaLaboratory for Regulation of Reproduction, College of Animal Sciences, Jilin University, Changchun 130062, ChinaLaboratory for Regulation of Reproduction, College of Animal Sciences, Jilin University, Changchun 130062, ChinaLaboratory for Regulation of Reproduction, College of Animal Sciences, Jilin University, Changchun 130062, ChinaLaboratory for Regulation of Reproduction, College of Animal Sciences, Jilin University, Changchun 130062, ChinaLaboratory for Regulation of Reproduction, College of Animal Sciences, Jilin University, Changchun 130062, ChinaAs a member of the neurotrophic family, brain-derived neurotrophic factor (BDNF) provides a key link in the physiological process of mammalian ovarian follicle development, in addition to its functions in the nervous system. The emphasis of this study lay in the impact of BDNF on the proliferation of porcine follicular granulosa cells (GCs) in vitro. BDNF and tyrosine kinase B (TrkB, receptor of BDNF) were detected in porcine follicular GCs. Additionally, cell viability significantly increased during the culture of porcine GCs with BDNF (100 ng/mL) in vitro. However, BDNF knockdown in GCs decreased cell viability and S-phase cells proportion—and BDNF simultaneously regulated the expression of genes linked with cell proliferation (CCND1, p21 and Bcl2) and apoptosis (Bax). Then, the results of the receptor blocking experiment showed that BDNF promoted GC proliferation via TrkB. The high-throughput sequencing showed that BDNF also regulated the expression profiles of miRNAs in GCs. The differential expression profiles were obtained by miRNA sequencing after BDNF (100 ng/mL) treatment with GCs. The sequencing results showed that, after BDNF treatment, 72 significant differentially-expressed miRNAs were detected—5 of which were related to cell process and proliferation signaling pathways confirmed by RT-PCR. Furthermore, studies showed that BDNF promoted GCs’ proliferation by increasing the expression of CCND1, downregulating miR-127 and activating the ERK1/2 signal pathway. Moreover, BDNF indirectly activated the ERK1/2 signal pathway by downregulating miR-127. In conclusion, BDNF promoted porcine GC proliferation by increasing CCND1 expression, downregulating miR-127 and stimulating the MAPK-ERK1/2 signaling cascade.https://www.mdpi.com/2076-2615/13/6/1115BDNFporcineproliferationmicroRNACCND1ERK |
| spellingShingle | Xue Zheng Lu Chen Tong Chen Maosheng Cao Boqi Zhang Chenfeng Yuan Zijiao Zhao Chunjin Li Xu Zhou The Mechanisms of BDNF Promoting the Proliferation of Porcine Follicular Granulosa Cells: Role of miR-127 and Involvement of the MAPK-ERK1/2 Pathway Animals BDNF porcine proliferation microRNA CCND1 ERK |
| title | The Mechanisms of BDNF Promoting the Proliferation of Porcine Follicular Granulosa Cells: Role of miR-127 and Involvement of the MAPK-ERK1/2 Pathway |
| title_full | The Mechanisms of BDNF Promoting the Proliferation of Porcine Follicular Granulosa Cells: Role of miR-127 and Involvement of the MAPK-ERK1/2 Pathway |
| title_fullStr | The Mechanisms of BDNF Promoting the Proliferation of Porcine Follicular Granulosa Cells: Role of miR-127 and Involvement of the MAPK-ERK1/2 Pathway |
| title_full_unstemmed | The Mechanisms of BDNF Promoting the Proliferation of Porcine Follicular Granulosa Cells: Role of miR-127 and Involvement of the MAPK-ERK1/2 Pathway |
| title_short | The Mechanisms of BDNF Promoting the Proliferation of Porcine Follicular Granulosa Cells: Role of miR-127 and Involvement of the MAPK-ERK1/2 Pathway |
| title_sort | mechanisms of bdnf promoting the proliferation of porcine follicular granulosa cells role of mir 127 and involvement of the mapk erk1 2 pathway |
| topic | BDNF porcine proliferation microRNA CCND1 ERK |
| url | https://www.mdpi.com/2076-2615/13/6/1115 |
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