Metagenomics Analysis of Microbial Species and Antibiotic Resistance Genes (ARGs) in Untreated Wastewater from Different Types of Hospitals in Hangzhou
Antibiotic resistance genes (ARGs) are contaminants that can propagate through a variety of environmental media. They contribute to an increase in the number of antibiotic-resistant microbes and thereby pose a danger to human health. Discharge from hospitals is the most significant contributor of AR...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2022-01-01
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| Series: | Adsorption Science & Technology |
| Online Access: | http://dx.doi.org/10.1155/2022/3344026 |
| _version_ | 1797281500431908864 |
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| author | Song Chen Yi Zhou Lu Song Yanli Yang Fuming He Qianming Chen Xi Cheng |
| author_facet | Song Chen Yi Zhou Lu Song Yanli Yang Fuming He Qianming Chen Xi Cheng |
| author_sort | Song Chen |
| collection | DOAJ |
| description | Antibiotic resistance genes (ARGs) are contaminants that can propagate through a variety of environmental media. They contribute to an increase in the number of antibiotic-resistant microbes and thereby pose a danger to human health. Discharge from hospitals is the most significant contributor of ARGs and antibiotic-resistant bacteria to the natural environment. A comprehensive understanding of the microbiological structure and the distribution of ARGs in hospital wastewater can facilitate appropriate treatment of such wastewater and can improve our understanding of the pathophysiology of several epidemic illnesses. In this work, metagenomics techniques were used to compare the microbial species and ARGs in a control group with those in untreated wastewater from three types of hospitals (a general hospital, a hospital of traditional Chinese medicine, and an oral specialty hospital). The microbiota found in the hospital wastewater represented 6,415 species and 244 phyla. The composition of the bacterial community in the wastewater from the three hospitals was significantly different from that in the control group. The ARGs in the samples were also analyzed using the Antibiotic Resistance Genes Database (ARDB) and Comprehensive Antibiotic Resistance Database (CARD). Finally, the link between abundant species and ARGs in the samples was examined. The findings of this study indicate that a connection exists between the microbial species and the ARG composition found in the wastewater samples. A variety of distinct genera of ARGs, each having their own unique correlations, have been found in wastewater from various hospitals. Consequently, the ARGs and microbial species found in the untreated wastewater from various hospitals have unique characteristics. Therefore, more detailed protocols need to be established to treat wastewater from various types of hospitals. Further studies should examine whether a connection exists between the various microbial species found in the wastewater of various types of hospitals and certain illnesses. |
| first_indexed | 2024-03-07T16:58:29Z |
| format | Article |
| id | doaj.art-da5c7c2aa04b40d79604f4f1a77fc55f |
| institution | Directory Open Access Journal |
| issn | 2048-4038 |
| language | English |
| last_indexed | 2024-03-07T16:58:29Z |
| publishDate | 2022-01-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Adsorption Science & Technology |
| spelling | doaj.art-da5c7c2aa04b40d79604f4f1a77fc55f2024-03-03T03:39:01ZengSAGE PublicationsAdsorption Science & Technology2048-40382022-01-01202210.1155/2022/3344026Metagenomics Analysis of Microbial Species and Antibiotic Resistance Genes (ARGs) in Untreated Wastewater from Different Types of Hospitals in HangzhouSong Chen0Yi Zhou1Lu Song2Yanli Yang3Fuming He4Qianming Chen5Xi Cheng6Stomatology HospitalStomatology HospitalStomatology HospitalStomatology HospitalStomatology HospitalStomatology HospitalStomatology HospitalAntibiotic resistance genes (ARGs) are contaminants that can propagate through a variety of environmental media. They contribute to an increase in the number of antibiotic-resistant microbes and thereby pose a danger to human health. Discharge from hospitals is the most significant contributor of ARGs and antibiotic-resistant bacteria to the natural environment. A comprehensive understanding of the microbiological structure and the distribution of ARGs in hospital wastewater can facilitate appropriate treatment of such wastewater and can improve our understanding of the pathophysiology of several epidemic illnesses. In this work, metagenomics techniques were used to compare the microbial species and ARGs in a control group with those in untreated wastewater from three types of hospitals (a general hospital, a hospital of traditional Chinese medicine, and an oral specialty hospital). The microbiota found in the hospital wastewater represented 6,415 species and 244 phyla. The composition of the bacterial community in the wastewater from the three hospitals was significantly different from that in the control group. The ARGs in the samples were also analyzed using the Antibiotic Resistance Genes Database (ARDB) and Comprehensive Antibiotic Resistance Database (CARD). Finally, the link between abundant species and ARGs in the samples was examined. The findings of this study indicate that a connection exists between the microbial species and the ARG composition found in the wastewater samples. A variety of distinct genera of ARGs, each having their own unique correlations, have been found in wastewater from various hospitals. Consequently, the ARGs and microbial species found in the untreated wastewater from various hospitals have unique characteristics. Therefore, more detailed protocols need to be established to treat wastewater from various types of hospitals. Further studies should examine whether a connection exists between the various microbial species found in the wastewater of various types of hospitals and certain illnesses.http://dx.doi.org/10.1155/2022/3344026 |
| spellingShingle | Song Chen Yi Zhou Lu Song Yanli Yang Fuming He Qianming Chen Xi Cheng Metagenomics Analysis of Microbial Species and Antibiotic Resistance Genes (ARGs) in Untreated Wastewater from Different Types of Hospitals in Hangzhou Adsorption Science & Technology |
| title | Metagenomics Analysis of Microbial Species and Antibiotic Resistance Genes (ARGs) in Untreated Wastewater from Different Types of Hospitals in Hangzhou |
| title_full | Metagenomics Analysis of Microbial Species and Antibiotic Resistance Genes (ARGs) in Untreated Wastewater from Different Types of Hospitals in Hangzhou |
| title_fullStr | Metagenomics Analysis of Microbial Species and Antibiotic Resistance Genes (ARGs) in Untreated Wastewater from Different Types of Hospitals in Hangzhou |
| title_full_unstemmed | Metagenomics Analysis of Microbial Species and Antibiotic Resistance Genes (ARGs) in Untreated Wastewater from Different Types of Hospitals in Hangzhou |
| title_short | Metagenomics Analysis of Microbial Species and Antibiotic Resistance Genes (ARGs) in Untreated Wastewater from Different Types of Hospitals in Hangzhou |
| title_sort | metagenomics analysis of microbial species and antibiotic resistance genes args in untreated wastewater from different types of hospitals in hangzhou |
| url | http://dx.doi.org/10.1155/2022/3344026 |
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