Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation
Background: Endometriosis is a common benign, estrogen-dependent, and chronic gynecological disease. Immune system disturbances and inflammatory abnormalities were involved in the pathogenesis of endometriosis. , Therefore, it is logical to use vitamin D, which has an immunomodulatory capacity, as s...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Tripoli University
2022-12-01
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| Series: | Open Veterinary Journal |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=115890 |
| _version_ | 1797958441873965056 |
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| author | Alfi Ruham Burjiah Ashon Sa'adi Widjiati Widjiati |
| author_facet | Alfi Ruham Burjiah Ashon Sa'adi Widjiati Widjiati |
| author_sort | Alfi Ruham Burjiah |
| collection | DOAJ |
| description | Background: Endometriosis is a common benign, estrogen-dependent, and chronic gynecological disease. Immune system disturbances and inflammatory abnormalities were involved in the pathogenesis of endometriosis. , Therefore, it is logical to use vitamin D, which has an immunomodulatory capacity, as supportive therapy for endometriosis.
Aims: This research time to study the effect of different doses of vitamin D on IL-17 expression in endometriosis mice models.
Methods: Endometriosis was induced in twenty-four mice divided into four groups of six. Group C received no treatment, while groups T1, T2, and T3 received graded doses of vitamin D to the stomach, sequentially 8 IU, 16 IU, and 24 IU for three weeks. Interleukin-17 expression and the extent of endometriotic peritoneal lesions were also measured and analyzed. Statistical tests were performed to see the difference in the mean area of endometriosis lesions and IL-17 expression between the control and treatment groups, as well as the correlation between the extent of endometriosis lesions and IL-17. We performed a statistical test to determine whether stratified doses have different IL-17 and endometriosis area outcomes.
Results: The area of endometriosis lesions was decreased after 16 IU and 24 IU of vitamin D administration (p 0.023 and 0.009). Endometriosis lesions also tended to be smaller after 8 IU of vitamin D supplementation, although insignificant (p > 0.05). Interleukin-17 expression was significantly lower after 24 IU vitamin D administration (p = 0.004). Vitamin D doses of 8 IU and 16 IU caused lower IL-17 expression, although not significant (p = 0.452 and p = 0.645). The regulation of IL-17 expression was significantly dependent on the dose level of vitamin D with an optimal dose of 24 IU (p = 0.004). This dose also caused the smallest endometriosis lesion, although not optimal (p > 0.05). IL-17 expression was moderately and positively correlated with the extent of endometriosis lesions (p = 0.012, rho = 0.505).
Conclusion: By modulating the expression of interleukin-17 in endometriotic lesions, vitamin D inhibits the development of endometriotic lesions in the endometriosis mice model. The optimal vitamin D dose in this study was 24 IU. [Open Vet J 2022; 12(6.000): 956-964] |
| first_indexed | 2024-04-11T00:19:03Z |
| format | Article |
| id | doaj.art-da5f39605dbe49e28dcde4955bd813f0 |
| institution | Directory Open Access Journal |
| issn | 2226-4485 |
| language | English |
| last_indexed | 2024-04-11T00:19:03Z |
| publishDate | 2022-12-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj.art-da5f39605dbe49e28dcde4955bd813f02023-01-08T13:33:56ZengTripoli UniversityOpen Veterinary Journal2226-44852022-12-0112695696410.5455/OVJ.2022.v12.i6.23115890Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulationAlfi Ruham Burjiah0Ashon Sa'adi1Widjiati Widjiati2Faculty of Medicine, Universitas Airlangga, Surabaya-60132 IndonesiaFaculty of Medicine, Universitas Airlangga, Surabaya-60132 IndonesiaFaculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115 IndonesiaBackground: Endometriosis is a common benign, estrogen-dependent, and chronic gynecological disease. Immune system disturbances and inflammatory abnormalities were involved in the pathogenesis of endometriosis. , Therefore, it is logical to use vitamin D, which has an immunomodulatory capacity, as supportive therapy for endometriosis. Aims: This research time to study the effect of different doses of vitamin D on IL-17 expression in endometriosis mice models. Methods: Endometriosis was induced in twenty-four mice divided into four groups of six. Group C received no treatment, while groups T1, T2, and T3 received graded doses of vitamin D to the stomach, sequentially 8 IU, 16 IU, and 24 IU for three weeks. Interleukin-17 expression and the extent of endometriotic peritoneal lesions were also measured and analyzed. Statistical tests were performed to see the difference in the mean area of endometriosis lesions and IL-17 expression between the control and treatment groups, as well as the correlation between the extent of endometriosis lesions and IL-17. We performed a statistical test to determine whether stratified doses have different IL-17 and endometriosis area outcomes. Results: The area of endometriosis lesions was decreased after 16 IU and 24 IU of vitamin D administration (p 0.023 and 0.009). Endometriosis lesions also tended to be smaller after 8 IU of vitamin D supplementation, although insignificant (p > 0.05). Interleukin-17 expression was significantly lower after 24 IU vitamin D administration (p = 0.004). Vitamin D doses of 8 IU and 16 IU caused lower IL-17 expression, although not significant (p = 0.452 and p = 0.645). The regulation of IL-17 expression was significantly dependent on the dose level of vitamin D with an optimal dose of 24 IU (p = 0.004). This dose also caused the smallest endometriosis lesion, although not optimal (p > 0.05). IL-17 expression was moderately and positively correlated with the extent of endometriosis lesions (p = 0.012, rho = 0.505). Conclusion: By modulating the expression of interleukin-17 in endometriotic lesions, vitamin D inhibits the development of endometriotic lesions in the endometriosis mice model. The optimal vitamin D dose in this study was 24 IU. [Open Vet J 2022; 12(6.000): 956-964]http://www.ejmanager.com/fulltextpdf.php?mno=115890endometriosis lesion sizeinterleukin 17reproductive healthvitamin d |
| spellingShingle | Alfi Ruham Burjiah Ashon Sa'adi Widjiati Widjiati Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation Open Veterinary Journal endometriosis lesion size interleukin 17 reproductive health vitamin d |
| title | Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation |
| title_full | Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation |
| title_fullStr | Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation |
| title_full_unstemmed | Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation |
| title_short | Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation |
| title_sort | vitamin d inhibited endometriosis development in mice model through interleukin 17 modulation |
| topic | endometriosis lesion size interleukin 17 reproductive health vitamin d |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=115890 |
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