Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates
Background: Microbial colonization of a neonate's gastrointestinal tract has significant perinatal and lifetime health consequences. However, information regarding the profile of meconium microbiota in neonates and the influence of clinical parameters are lacking in the Indonesian population. T...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020324191 |
| _version_ | 1819296518913392640 |
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| author | Kristien Juni Thandwi Jonathan Grasella Ong Firsty Amanah Prasetyaningsih Radhian Amandito Rinawati Rohsiswatmo Amarila Malik |
| author_facet | Kristien Juni Thandwi Jonathan Grasella Ong Firsty Amanah Prasetyaningsih Radhian Amandito Rinawati Rohsiswatmo Amarila Malik |
| author_sort | Kristien Juni Thandwi Jonathan |
| collection | DOAJ |
| description | Background: Microbial colonization of a neonate's gastrointestinal tract has significant perinatal and lifetime health consequences. However, information regarding the profile of meconium microbiota in neonates and the influence of clinical parameters are lacking in the Indonesian population. This study aimed to preliminary investigate the profile of cultivable bacterial diversity of meconium isolated from neonates born at Cipto Mangunkusumo Hospital (CMH), Jakarta. The cultivable bacteria were isolated from meconium samples and were then processed for cultivation and molecular identification. Results: Fourteen neonates were enrolled as described, i.e., seven hyperbilirubinemia (Hyp) and seven non-Hyp with ten neonates delivered by cesarean section (CS) and four others by vaginal route (VR), and with five exclusive breastfeeding (Ebf), four formula milk, and five combinations. Microbiological identification, molecular 16S rDNA PCR-Sanger sequencing, and PCA analysis of cultivable bacteria isolated from meconium showed Firmicutes' predominance (84.41%), with an abundant population of Staphylococcus, which consist of S. hominis, S. epidermidis, and S. haemolyticus. The influence of mode of delivery showed a lower diversity than the CS populates the VR, but their composition was similar. Concurrently, between feeding patterns, the genera profile did not show much difference; in the non-Ebf group, the total amount of Staphylococcus and Bacillus showed a higher amount but a less diverse. Interestingly, the non-Hyp group showed more abundant and diverse Staphylococcus than that of the Hyp group. In contrast, neonates diagnosed with NEC and proven sepsis showed the same pattern of Staphylococcus domination. Conclusion: Staphylococcus predominated the composition of cultivable bacteria in neonates meconium. Due to the small sample size, only the hyperbilirubinemia parameter significantly influenced the profile, i.e., Staphylococcus's proportion (p = 0.037). |
| first_indexed | 2024-12-24T04:59:23Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-24T04:59:23Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-da600619729a43298a9288f2bc071f312022-12-21T17:14:18ZengElsevierHeliyon2405-84402020-12-01612e05576Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonatesKristien Juni Thandwi Jonathan0Grasella Ong1Firsty Amanah Prasetyaningsih2Radhian Amandito3Rinawati Rohsiswatmo4Amarila Malik5Division of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia, UI Depok Campus, Depok 16424, IndonesiaDivision of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia, UI Depok Campus, Depok 16424, IndonesiaDivision of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia, UI Depok Campus, Depok 16424, IndonesiaDivision of Perinatology, Department of Child Health, Cipto Mangunkusumo Hospital-Faculty of Medicine, Universitas Indonesia, Jl Pangeran Diponegoro No. 71, Jakarta 10430, Indonesia; Corresponding author.Division of Perinatology, Department of Child Health, Cipto Mangunkusumo Hospital-Faculty of Medicine, Universitas Indonesia, Jl Pangeran Diponegoro No. 71, Jakarta 10430, IndonesiaDivision of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia, UI Depok Campus, Depok 16424, Indonesia; Corresponding author.Background: Microbial colonization of a neonate's gastrointestinal tract has significant perinatal and lifetime health consequences. However, information regarding the profile of meconium microbiota in neonates and the influence of clinical parameters are lacking in the Indonesian population. This study aimed to preliminary investigate the profile of cultivable bacterial diversity of meconium isolated from neonates born at Cipto Mangunkusumo Hospital (CMH), Jakarta. The cultivable bacteria were isolated from meconium samples and were then processed for cultivation and molecular identification. Results: Fourteen neonates were enrolled as described, i.e., seven hyperbilirubinemia (Hyp) and seven non-Hyp with ten neonates delivered by cesarean section (CS) and four others by vaginal route (VR), and with five exclusive breastfeeding (Ebf), four formula milk, and five combinations. Microbiological identification, molecular 16S rDNA PCR-Sanger sequencing, and PCA analysis of cultivable bacteria isolated from meconium showed Firmicutes' predominance (84.41%), with an abundant population of Staphylococcus, which consist of S. hominis, S. epidermidis, and S. haemolyticus. The influence of mode of delivery showed a lower diversity than the CS populates the VR, but their composition was similar. Concurrently, between feeding patterns, the genera profile did not show much difference; in the non-Ebf group, the total amount of Staphylococcus and Bacillus showed a higher amount but a less diverse. Interestingly, the non-Hyp group showed more abundant and diverse Staphylococcus than that of the Hyp group. In contrast, neonates diagnosed with NEC and proven sepsis showed the same pattern of Staphylococcus domination. Conclusion: Staphylococcus predominated the composition of cultivable bacteria in neonates meconium. Due to the small sample size, only the hyperbilirubinemia parameter significantly influenced the profile, i.e., Staphylococcus's proportion (p = 0.037).http://www.sciencedirect.com/science/article/pii/S2405844020324191MicrobiologyClinical researchDigestive systemGastrointestinal systemHealth sciencesPediatrics |
| spellingShingle | Kristien Juni Thandwi Jonathan Grasella Ong Firsty Amanah Prasetyaningsih Radhian Amandito Rinawati Rohsiswatmo Amarila Malik Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates Heliyon Microbiology Clinical research Digestive system Gastrointestinal system Health sciences Pediatrics |
| title | Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates |
| title_full | Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates |
| title_fullStr | Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates |
| title_full_unstemmed | Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates |
| title_short | Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates |
| title_sort | clinical characteristics influence cultivable bacteria composition in the meconium of indonesian neonates |
| topic | Microbiology Clinical research Digestive system Gastrointestinal system Health sciences Pediatrics |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020324191 |
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