Metabolic labeling of enterovirus 71 with quantum dots for the study of virus receptor usage
Abstract Fluorescent labeling and dynamic tracking is a powerful tool for exploring virus infection mechanisms. However, for small-sized viruses, virus tracking studies are usually hindered by a lack of appropriate labeling methods that do not dampen virus yield or infectivity. Here, we report a uni...
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-021-01046-5 |
| _version_ | 1798025119503745024 |
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| author | Xianliang Ke Chunjie Li Dan Luo Ting Wang Yan Liu Zhongyuan Tan Mingyuan Du Zhike He Hanzhong Wang Zhenhua Zheng Yuan Zhang |
| author_facet | Xianliang Ke Chunjie Li Dan Luo Ting Wang Yan Liu Zhongyuan Tan Mingyuan Du Zhike He Hanzhong Wang Zhenhua Zheng Yuan Zhang |
| author_sort | Xianliang Ke |
| collection | DOAJ |
| description | Abstract Fluorescent labeling and dynamic tracking is a powerful tool for exploring virus infection mechanisms. However, for small-sized viruses, virus tracking studies are usually hindered by a lack of appropriate labeling methods that do not dampen virus yield or infectivity. Here, we report a universal strategy for labeling viruses with chemical dyes and Quantum dots (QDs). Enterovirus 71 (EV71) was produced in a cell line that stably expresses a mutant methionyl-tRNA synthetase (MetRS), which can charge azidonorleucine (ANL) to the methionine sites of viral proteins during translation. Then, the ANL-containing virus was easily labeled with DBCO-AF647 and DBCO-QDs. The labeled virus shows sufficient yield and no obvious decrease in infectivity and can be used for imaging the virus entry process. Using the labeled EV71, different functions of scavenger receptor class B, member 2 (SCARB2), and heparan sulfate (HS) in EV71 infection were comparatively studied. The cell entry process of a strong HS-binding EV71 strain was investigated by real-time dynamic visualization of EV71-QDs in living cells. Taken together, our study described a universal biocompatible virus labeling method, visualized the dynamic viral entry process, and reported details of the receptor usage of EV71. Graphic Abstract |
| first_indexed | 2024-04-11T18:13:37Z |
| format | Article |
| id | doaj.art-da60830914a54d428a5d5cd6a3448fb7 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T18:13:37Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-da60830914a54d428a5d5cd6a3448fb72022-12-22T04:10:01ZengBMCJournal of Nanobiotechnology1477-31552021-09-0119111210.1186/s12951-021-01046-5Metabolic labeling of enterovirus 71 with quantum dots for the study of virus receptor usageXianliang Ke0Chunjie Li1Dan Luo2Ting Wang3Yan Liu4Zhongyuan Tan5Mingyuan Du6Zhike He7Hanzhong Wang8Zhenhua Zheng9Yuan Zhang10CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of SciencesCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of SciencesDepartment of Gastroenterology, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and TechnologyThe Center for Biomedical Research, Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and TechnologyCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of SciencesCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of SciencesCollege of Chemistry and Molecular Sciences, Wuhan UniversityCollege of Chemistry and Molecular Sciences, Wuhan UniversityCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of SciencesCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of SciencesCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of SciencesAbstract Fluorescent labeling and dynamic tracking is a powerful tool for exploring virus infection mechanisms. However, for small-sized viruses, virus tracking studies are usually hindered by a lack of appropriate labeling methods that do not dampen virus yield or infectivity. Here, we report a universal strategy for labeling viruses with chemical dyes and Quantum dots (QDs). Enterovirus 71 (EV71) was produced in a cell line that stably expresses a mutant methionyl-tRNA synthetase (MetRS), which can charge azidonorleucine (ANL) to the methionine sites of viral proteins during translation. Then, the ANL-containing virus was easily labeled with DBCO-AF647 and DBCO-QDs. The labeled virus shows sufficient yield and no obvious decrease in infectivity and can be used for imaging the virus entry process. Using the labeled EV71, different functions of scavenger receptor class B, member 2 (SCARB2), and heparan sulfate (HS) in EV71 infection were comparatively studied. The cell entry process of a strong HS-binding EV71 strain was investigated by real-time dynamic visualization of EV71-QDs in living cells. Taken together, our study described a universal biocompatible virus labeling method, visualized the dynamic viral entry process, and reported details of the receptor usage of EV71. Graphic Abstracthttps://doi.org/10.1186/s12951-021-01046-5EV71Quantum dotsNanobiotechnologyMetabolic labelingANLHeparan sulfate |
| spellingShingle | Xianliang Ke Chunjie Li Dan Luo Ting Wang Yan Liu Zhongyuan Tan Mingyuan Du Zhike He Hanzhong Wang Zhenhua Zheng Yuan Zhang Metabolic labeling of enterovirus 71 with quantum dots for the study of virus receptor usage Journal of Nanobiotechnology EV71 Quantum dots Nanobiotechnology Metabolic labeling ANL Heparan sulfate |
| title | Metabolic labeling of enterovirus 71 with quantum dots for the study of virus receptor usage |
| title_full | Metabolic labeling of enterovirus 71 with quantum dots for the study of virus receptor usage |
| title_fullStr | Metabolic labeling of enterovirus 71 with quantum dots for the study of virus receptor usage |
| title_full_unstemmed | Metabolic labeling of enterovirus 71 with quantum dots for the study of virus receptor usage |
| title_short | Metabolic labeling of enterovirus 71 with quantum dots for the study of virus receptor usage |
| title_sort | metabolic labeling of enterovirus 71 with quantum dots for the study of virus receptor usage |
| topic | EV71 Quantum dots Nanobiotechnology Metabolic labeling ANL Heparan sulfate |
| url | https://doi.org/10.1186/s12951-021-01046-5 |
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