Variation in the Ovine Glycogen Synthase Kinase 3 Beta-Interaction Protein Gene (<i>GSKIP</i>) Affects Carcass and Growth Traits in Romney Sheep
The glycogen synthase kinase 3 beta (GSK3β)-interacting protein (encoded by the gene <i>GSKIP</i>) is a small A-kinase anchoring protein, which complexes with GSK3βand protein kinase A (PKA) and acts synergistically with cAMP/PKA signaling to inhibit GSK3β activity. The protein plays a r...
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MDPI AG
2021-09-01
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| author | Fangfang Zhao Huitong Zhou Shaobin Li Qingming An Qian Fang Yuzhu Luo Jon G. H. Hickford |
| author_facet | Fangfang Zhao Huitong Zhou Shaobin Li Qingming An Qian Fang Yuzhu Luo Jon G. H. Hickford |
| author_sort | Fangfang Zhao |
| collection | DOAJ |
| description | The glycogen synthase kinase 3 beta (GSK3β)-interacting protein (encoded by the gene <i>GSKIP</i>) is a small A-kinase anchoring protein, which complexes with GSK3βand protein kinase A (PKA) and acts synergistically with cAMP/PKA signaling to inhibit GSK3β activity. The protein plays a role in regulating glycogen metabolism, protein synthesis, the cell cycle, and in regulating gene expression. In this study, PCR-single strand conformation polymorphism (PCR-SSCP) analyses were used to screen for variation in exon 1 and exon 2 of <i>GSKIP</i> in 840 New Zealand (NZ) Romney sheep. Two SSCP banding patterns representing two different nucleotide variants (<i>A</i> and <i>B</i>) were detected in an exon 1 region, whereas in an exon 2 region only one pattern was detected. Variants <i>A</i> and <i>B</i> of exon 1 had one non-synonymous nucleotide difference c.37A/G (p.Met13Val). The birthweight of sheep of genotype <i>AA</i> (5.9 ± 0.06 kg) was different (<i>p</i> = 0.023) to sheep of genotype <i>AB</i> (5.7 ± 0.06 kg) and <i>BB</i> (5.7 ± 0.06 kg). The hot carcass weight (HCW) of sheep of genotype <i>AA</i> (17.2 ± 0.22 kg) was different (<i>p</i> = 0.012) to sheep of genotype <i>AB</i> (17.6 ± 0.22 kg) and <i>BB</i> (18.0 ± 0.29 kg), and the fat depth at the 12th rib (V-GR) of sheep of genotype <i>AA</i> (7.7 ± 0.31 mm) was different (<i>p</i> = 0.016) to sheep of genotype <i>AB</i> (8.3 ± 0.30 mm) and <i>BB</i> (8.5 ± 0.39 mm). The results suggest that the c.37A/G substitution in ovine <i>GSKIP</i> may affect sheep growth and carcass traits. |
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| spelling | doaj.art-da63afb682854e5eb8d0e8c3b980ae652023-11-22T11:44:02ZengMDPI AGAnimals2076-26152021-09-01119269010.3390/ani11092690Variation in the Ovine Glycogen Synthase Kinase 3 Beta-Interaction Protein Gene (<i>GSKIP</i>) Affects Carcass and Growth Traits in Romney SheepFangfang Zhao0Huitong Zhou1Shaobin Li2Qingming An3Qian Fang4Yuzhu Luo5Jon G. H. Hickford6Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaFaculty of Wujiang, Tongren University, Tongren 554300, ChinaInternational Science and Technology Cooperation Base of Meat Sheep and Meat Cattle Genetic Improvement in Northwest of China, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaInternational Science and Technology Cooperation Base of Meat Sheep and Meat Cattle Genetic Improvement in Northwest of China, Gansu Agricultural University, Lanzhou 730070, ChinaThe glycogen synthase kinase 3 beta (GSK3β)-interacting protein (encoded by the gene <i>GSKIP</i>) is a small A-kinase anchoring protein, which complexes with GSK3βand protein kinase A (PKA) and acts synergistically with cAMP/PKA signaling to inhibit GSK3β activity. The protein plays a role in regulating glycogen metabolism, protein synthesis, the cell cycle, and in regulating gene expression. In this study, PCR-single strand conformation polymorphism (PCR-SSCP) analyses were used to screen for variation in exon 1 and exon 2 of <i>GSKIP</i> in 840 New Zealand (NZ) Romney sheep. Two SSCP banding patterns representing two different nucleotide variants (<i>A</i> and <i>B</i>) were detected in an exon 1 region, whereas in an exon 2 region only one pattern was detected. Variants <i>A</i> and <i>B</i> of exon 1 had one non-synonymous nucleotide difference c.37A/G (p.Met13Val). The birthweight of sheep of genotype <i>AA</i> (5.9 ± 0.06 kg) was different (<i>p</i> = 0.023) to sheep of genotype <i>AB</i> (5.7 ± 0.06 kg) and <i>BB</i> (5.7 ± 0.06 kg). The hot carcass weight (HCW) of sheep of genotype <i>AA</i> (17.2 ± 0.22 kg) was different (<i>p</i> = 0.012) to sheep of genotype <i>AB</i> (17.6 ± 0.22 kg) and <i>BB</i> (18.0 ± 0.29 kg), and the fat depth at the 12th rib (V-GR) of sheep of genotype <i>AA</i> (7.7 ± 0.31 mm) was different (<i>p</i> = 0.016) to sheep of genotype <i>AB</i> (8.3 ± 0.30 mm) and <i>BB</i> (8.5 ± 0.39 mm). The results suggest that the c.37A/G substitution in ovine <i>GSKIP</i> may affect sheep growth and carcass traits.https://www.mdpi.com/2076-2615/11/9/2690NZ Romney sheepglycogen synthase kinase 3 beta-interacting protein gene (<i>GSKIP</i>)nucleotide substitutionovine carcass traitsovine growth |
| spellingShingle | Fangfang Zhao Huitong Zhou Shaobin Li Qingming An Qian Fang Yuzhu Luo Jon G. H. Hickford Variation in the Ovine Glycogen Synthase Kinase 3 Beta-Interaction Protein Gene (<i>GSKIP</i>) Affects Carcass and Growth Traits in Romney Sheep Animals NZ Romney sheep glycogen synthase kinase 3 beta-interacting protein gene (<i>GSKIP</i>) nucleotide substitution ovine carcass traits ovine growth |
| title | Variation in the Ovine Glycogen Synthase Kinase 3 Beta-Interaction Protein Gene (<i>GSKIP</i>) Affects Carcass and Growth Traits in Romney Sheep |
| title_full | Variation in the Ovine Glycogen Synthase Kinase 3 Beta-Interaction Protein Gene (<i>GSKIP</i>) Affects Carcass and Growth Traits in Romney Sheep |
| title_fullStr | Variation in the Ovine Glycogen Synthase Kinase 3 Beta-Interaction Protein Gene (<i>GSKIP</i>) Affects Carcass and Growth Traits in Romney Sheep |
| title_full_unstemmed | Variation in the Ovine Glycogen Synthase Kinase 3 Beta-Interaction Protein Gene (<i>GSKIP</i>) Affects Carcass and Growth Traits in Romney Sheep |
| title_short | Variation in the Ovine Glycogen Synthase Kinase 3 Beta-Interaction Protein Gene (<i>GSKIP</i>) Affects Carcass and Growth Traits in Romney Sheep |
| title_sort | variation in the ovine glycogen synthase kinase 3 beta interaction protein gene i gskip i affects carcass and growth traits in romney sheep |
| topic | NZ Romney sheep glycogen synthase kinase 3 beta-interacting protein gene (<i>GSKIP</i>) nucleotide substitution ovine carcass traits ovine growth |
| url | https://www.mdpi.com/2076-2615/11/9/2690 |
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