Fibroblast Growth Factor 11 (FGF11) Promotes Progression and Cisplatin Resistance Through the HIF-1α/FGF11 Signaling Axis in Ovarian Clear Cell Carcinoma
Min Yin,1,* Chunli Lu,2,* Huimei Zhou,1 Qian Liu,1 Jiaxin Yang1 1National Clinical Research Center for Obstetric and Gynecologic Diseases, Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical Coll...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-07-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/fibroblast-growth-factor-11-fgf11-promotes-progression-and-cisplatin-r-peer-reviewed-fulltext-article-CMAR |
| _version_ | 1797771055536799744 |
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| author | Yin M Lu C Zhou H Liu Q Yang J |
| author_facet | Yin M Lu C Zhou H Liu Q Yang J |
| author_sort | Yin M |
| collection | DOAJ |
| description | Min Yin,1,* Chunli Lu,2,* Huimei Zhou,1 Qian Liu,1 Jiaxin Yang1 1National Clinical Research Center for Obstetric and Gynecologic Diseases, Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China; 2Neurospine Center, China International Neuroscience Institute (CHINA-INI), Xuanwu Hospital, Capital Medical University, National Center for Neurological Disorders, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Huimei Zhou; Jiaxin Yang, Email zhmpumch@126.com; yjxpumch@163.comBackground: A poor prognosis is often associated with ovarian clear cell carcinoma (OCCC) due to its relative resistance to platinum-based chemotherapy. Although several studies have been launched to explore the pathogenesis of OCCC, the mechanism of chemoresistance has yet to be uncovered.Methods: Nanostring nCounter PanCancer Pathways Panel was performed to explore the expression profiles of OCCC tissues from patients showing different platinum sensitivity. Bioinformatic analysis was performed to select genes associated with chemoresistance and cell function assays, including colony formation, wound healing, transwell and flow cytometric analysis, were used to explore the role of the target gene in the progression of OCCC and resistance to cisplatin (DDP).Results: Gene expression profiles and bioinformatic analysis verified that the expression of fibroblast growth factor 11 (FGF11) was significantly increased in platinum-resistant OCCC tissues and increased FGF11 expression was related to poorer survival. Downregulation of FGF11 inhibited the proliferation, migration, and invasion, reversing the DDP resistance of OCCC cells. Mechanically, FGF11 was regulated by hypoxia-inducible factor-1α (HIF-1α) to modulate the DDP sensitivity.Conclusion: FGF11 was highly expressed in platinum-resistant OCCC tissues, promoting progression and resistance to DDP through the HIF-1α/FGF11 signaling axis.Keywords: ovarian clear cell carcinoma, cisplatin chemoresistance, FGF11, HIF-1α |
| first_indexed | 2024-03-12T21:31:57Z |
| format | Article |
| id | doaj.art-da6bc7593de64c38805d9970207154ad |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-03-12T21:31:57Z |
| publishDate | 2023-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-da6bc7593de64c38805d9970207154ad2023-07-27T19:14:33ZengDove Medical PressCancer Management and Research1179-13222023-07-01Volume 1575376385444Fibroblast Growth Factor 11 (FGF11) Promotes Progression and Cisplatin Resistance Through the HIF-1α/FGF11 Signaling Axis in Ovarian Clear Cell CarcinomaYin MLu CZhou HLiu QYang JMin Yin,1,* Chunli Lu,2,* Huimei Zhou,1 Qian Liu,1 Jiaxin Yang1 1National Clinical Research Center for Obstetric and Gynecologic Diseases, Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China; 2Neurospine Center, China International Neuroscience Institute (CHINA-INI), Xuanwu Hospital, Capital Medical University, National Center for Neurological Disorders, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Huimei Zhou; Jiaxin Yang, Email zhmpumch@126.com; yjxpumch@163.comBackground: A poor prognosis is often associated with ovarian clear cell carcinoma (OCCC) due to its relative resistance to platinum-based chemotherapy. Although several studies have been launched to explore the pathogenesis of OCCC, the mechanism of chemoresistance has yet to be uncovered.Methods: Nanostring nCounter PanCancer Pathways Panel was performed to explore the expression profiles of OCCC tissues from patients showing different platinum sensitivity. Bioinformatic analysis was performed to select genes associated with chemoresistance and cell function assays, including colony formation, wound healing, transwell and flow cytometric analysis, were used to explore the role of the target gene in the progression of OCCC and resistance to cisplatin (DDP).Results: Gene expression profiles and bioinformatic analysis verified that the expression of fibroblast growth factor 11 (FGF11) was significantly increased in platinum-resistant OCCC tissues and increased FGF11 expression was related to poorer survival. Downregulation of FGF11 inhibited the proliferation, migration, and invasion, reversing the DDP resistance of OCCC cells. Mechanically, FGF11 was regulated by hypoxia-inducible factor-1α (HIF-1α) to modulate the DDP sensitivity.Conclusion: FGF11 was highly expressed in platinum-resistant OCCC tissues, promoting progression and resistance to DDP through the HIF-1α/FGF11 signaling axis.Keywords: ovarian clear cell carcinoma, cisplatin chemoresistance, FGF11, HIF-1αhttps://www.dovepress.com/fibroblast-growth-factor-11-fgf11-promotes-progression-and-cisplatin-r-peer-reviewed-fulltext-article-CMARovarian clear cell carcinomacisplatin chemoresistancefgf11hif-1α |
| spellingShingle | Yin M Lu C Zhou H Liu Q Yang J Fibroblast Growth Factor 11 (FGF11) Promotes Progression and Cisplatin Resistance Through the HIF-1α/FGF11 Signaling Axis in Ovarian Clear Cell Carcinoma Cancer Management and Research ovarian clear cell carcinoma cisplatin chemoresistance fgf11 hif-1α |
| title | Fibroblast Growth Factor 11 (FGF11) Promotes Progression and Cisplatin Resistance Through the HIF-1α/FGF11 Signaling Axis in Ovarian Clear Cell Carcinoma |
| title_full | Fibroblast Growth Factor 11 (FGF11) Promotes Progression and Cisplatin Resistance Through the HIF-1α/FGF11 Signaling Axis in Ovarian Clear Cell Carcinoma |
| title_fullStr | Fibroblast Growth Factor 11 (FGF11) Promotes Progression and Cisplatin Resistance Through the HIF-1α/FGF11 Signaling Axis in Ovarian Clear Cell Carcinoma |
| title_full_unstemmed | Fibroblast Growth Factor 11 (FGF11) Promotes Progression and Cisplatin Resistance Through the HIF-1α/FGF11 Signaling Axis in Ovarian Clear Cell Carcinoma |
| title_short | Fibroblast Growth Factor 11 (FGF11) Promotes Progression and Cisplatin Resistance Through the HIF-1α/FGF11 Signaling Axis in Ovarian Clear Cell Carcinoma |
| title_sort | fibroblast growth factor 11 fgf11 promotes progression and cisplatin resistance through the hif 1 alpha fgf11 signaling axis in ovarian clear cell carcinoma |
| topic | ovarian clear cell carcinoma cisplatin chemoresistance fgf11 hif-1α |
| url | https://www.dovepress.com/fibroblast-growth-factor-11-fgf11-promotes-progression-and-cisplatin-r-peer-reviewed-fulltext-article-CMAR |
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