Glyoxyl-Activated Agarose as Support for Covalently Link Novo-Pro D: Biocatalysts Performance in the Hydrolysis of Casein

Glyoxyl-Activated Agarose as Support for Covalently Link Novo-Pro D: Biocatalysts Performance in the Hydrolysis of Casein

This study aimed to evaluate the performance of a commercial protease (Novo-Pro D (NPD)), both in soluble and immobilized forms, in the hydrolysis of proteins (using casein as model protein). Immobilization of the protease NPD on 6% agarose activated with glyoxyl groups for 24 h at 20 °C and pH 10.0...

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Bibliographic Details
Main Authors: Laiane Antunes Lopes, Paula Kern Novelli, Roberto Fernandez-Lafuente, Paulo Waldir Tardioli, Raquel Lima Camargo Giordano
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Catalysts
Subjects:
enzyme immobilization
enzyme stabilization
protease
protein hydrolysis
agarose-glyoxyl
Online Access:https://www.mdpi.com/2073-4344/10/5/466

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