Chlorophyll Fluorescence Imaging as a Tool for Evaluating Disease Resistance of Common Bean Lines in the Western Amazon Region of Colombia
The evaluation of disease resistance is considered an important aspect of phenotyping for crop improvement. Identification of advanced lines of the common bean with disease resistance contributes to improved grain yields. This study aimed to determine the response of the photosynthetic apparatus to...
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MDPI AG
2022-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/10/1371 |
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| author | Juan Carlos Suárez José Iván Vanegas Amara Tatiana Contreras José Alexander Anzola Milan O. Urban Stephen E. Beebe Idupulapati M. Rao |
| author_facet | Juan Carlos Suárez José Iván Vanegas Amara Tatiana Contreras José Alexander Anzola Milan O. Urban Stephen E. Beebe Idupulapati M. Rao |
| author_sort | Juan Carlos Suárez |
| collection | DOAJ |
| description | The evaluation of disease resistance is considered an important aspect of phenotyping for crop improvement. Identification of advanced lines of the common bean with disease resistance contributes to improved grain yields. This study aimed to determine the response of the photosynthetic apparatus to natural pathogen infection by using chlorophyll (Chl<sub>a</sub>) fluorescence parameters and their relationship to the agronomic performance of 59 common bean lines and comparing the photosynthetic responses of naturally infected vs. healthy leaves. The study was conducted over two seasons under acid soil and high temperature conditions in the western Amazon region of Colombia. A disease susceptibility index (DSI) was developed and validated using chlorophyll a (Chl<sub>a</sub>) fluorescence as a tool to identify Mesoamerican and Andean lines of common bean (<i>Phaseolus vulgaris</i> L.) that are resistant to pathogens. A negative effect on the functional status of the photosynthetic apparatus was found with the presence of pathogen infection, a situation that allowed the identification of four typologies based on the DSI values ((i) moderately resistant; (ii) moderately susceptible; (iii) susceptible; and (iv) highly susceptible). Moderately resistant lines, five of them from the Mesoamerican gene pool (ALB 350, SMC 200, BFS 10, SER 16, SMN 27) and one from the Andean gene pool (DAB 295), allocated a higher proportion of energy to photochemical processes, which increased the rate of electron transfer resulting in a lower sensitivity to disease stress. This photosynthetic response was associated with lower values of DSI, which translated into an increase in the accumulation of dry matter accumulation in different plant organs (leaves, stem, pods and roots). Thus, DSI values based on chlorophyll fluorescence response to pathogen infection could serve as a phenotyping tool for evaluating advanced common bean lines. Six common bean lines (ALB 350, BFS 10, DAB 295, SER 16, SMC 200 and SMN 27) were identified as less sensitive to disease stress under field conditions in the western Amazon region of Colombia, and these could serve as useful parents for improving the common bean for multiple stress resistance. |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:03:09Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-da700e1d8d12443691e0f463934ca52b2023-11-23T12:43:34ZengMDPI AGPlants2223-77472022-05-011110137110.3390/plants11101371Chlorophyll Fluorescence Imaging as a Tool for Evaluating Disease Resistance of Common Bean Lines in the Western Amazon Region of ColombiaJuan Carlos Suárez0José Iván Vanegas1Amara Tatiana Contreras2José Alexander Anzola3Milan O. Urban4Stephen E. Beebe5Idupulapati M. Rao6Programa de Ingeniería Agroecológica, Facultad de Ingeniería, Universidad de la Amazonia, Florencia 180001, ColombiaPrograma de Ingeniería Agroecológica, Facultad de Ingeniería, Universidad de la Amazonia, Florencia 180001, ColombiaPrograma de Ingeniería Agroecológica, Facultad de Ingeniería, Universidad de la Amazonia, Florencia 180001, ColombiaPrograma de Ingeniería Agroecológica, Facultad de Ingeniería, Universidad de la Amazonia, Florencia 180001, ColombiaInternational Center for Tropical Agriculture (CIAT), Km 17 Recta Cali-Palmira, Cali 763537, ColombiaInternational Center for Tropical Agriculture (CIAT), Km 17 Recta Cali-Palmira, Cali 763537, ColombiaInternational Center for Tropical Agriculture (CIAT), Km 17 Recta Cali-Palmira, Cali 763537, ColombiaThe evaluation of disease resistance is considered an important aspect of phenotyping for crop improvement. Identification of advanced lines of the common bean with disease resistance contributes to improved grain yields. This study aimed to determine the response of the photosynthetic apparatus to natural pathogen infection by using chlorophyll (Chl<sub>a</sub>) fluorescence parameters and their relationship to the agronomic performance of 59 common bean lines and comparing the photosynthetic responses of naturally infected vs. healthy leaves. The study was conducted over two seasons under acid soil and high temperature conditions in the western Amazon region of Colombia. A disease susceptibility index (DSI) was developed and validated using chlorophyll a (Chl<sub>a</sub>) fluorescence as a tool to identify Mesoamerican and Andean lines of common bean (<i>Phaseolus vulgaris</i> L.) that are resistant to pathogens. A negative effect on the functional status of the photosynthetic apparatus was found with the presence of pathogen infection, a situation that allowed the identification of four typologies based on the DSI values ((i) moderately resistant; (ii) moderately susceptible; (iii) susceptible; and (iv) highly susceptible). Moderately resistant lines, five of them from the Mesoamerican gene pool (ALB 350, SMC 200, BFS 10, SER 16, SMN 27) and one from the Andean gene pool (DAB 295), allocated a higher proportion of energy to photochemical processes, which increased the rate of electron transfer resulting in a lower sensitivity to disease stress. This photosynthetic response was associated with lower values of DSI, which translated into an increase in the accumulation of dry matter accumulation in different plant organs (leaves, stem, pods and roots). Thus, DSI values based on chlorophyll fluorescence response to pathogen infection could serve as a phenotyping tool for evaluating advanced common bean lines. Six common bean lines (ALB 350, BFS 10, DAB 295, SER 16, SMC 200 and SMN 27) were identified as less sensitive to disease stress under field conditions in the western Amazon region of Colombia, and these could serve as useful parents for improving the common bean for multiple stress resistance.https://www.mdpi.com/2223-7747/11/10/1371acid soilagronomic performancedisease susceptibility indexdry matter accumulationgrain yieldhigh temperature |
| spellingShingle | Juan Carlos Suárez José Iván Vanegas Amara Tatiana Contreras José Alexander Anzola Milan O. Urban Stephen E. Beebe Idupulapati M. Rao Chlorophyll Fluorescence Imaging as a Tool for Evaluating Disease Resistance of Common Bean Lines in the Western Amazon Region of Colombia Plants acid soil agronomic performance disease susceptibility index dry matter accumulation grain yield high temperature |
| title | Chlorophyll Fluorescence Imaging as a Tool for Evaluating Disease Resistance of Common Bean Lines in the Western Amazon Region of Colombia |
| title_full | Chlorophyll Fluorescence Imaging as a Tool for Evaluating Disease Resistance of Common Bean Lines in the Western Amazon Region of Colombia |
| title_fullStr | Chlorophyll Fluorescence Imaging as a Tool for Evaluating Disease Resistance of Common Bean Lines in the Western Amazon Region of Colombia |
| title_full_unstemmed | Chlorophyll Fluorescence Imaging as a Tool for Evaluating Disease Resistance of Common Bean Lines in the Western Amazon Region of Colombia |
| title_short | Chlorophyll Fluorescence Imaging as a Tool for Evaluating Disease Resistance of Common Bean Lines in the Western Amazon Region of Colombia |
| title_sort | chlorophyll fluorescence imaging as a tool for evaluating disease resistance of common bean lines in the western amazon region of colombia |
| topic | acid soil agronomic performance disease susceptibility index dry matter accumulation grain yield high temperature |
| url | https://www.mdpi.com/2223-7747/11/10/1371 |
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