Effect of growth regulators and explant origin on in vitro propagation of Ceratonia siliqua L. via cuttings
The present work was undertaken to develop a basic and simple protocol for micropropagation of Ceratonia siliqua. Axillaries bud sprouting and shoot development were stimulated on MS supplemented with BAP (0.5 mg.l-1), IBA (0.1 mg.l-1) and GA3 (0.5 mg.l-1), shoot multiplication was obtained on MS su...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Presses Agronomiques de Gembloux
2008-01-01
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| Series: | Biotechnologie, Agronomie, Société et Environnement |
| Subjects: | |
| Online Access: | http://www.pressesagro.be/base/text/v12n3/251.pdf |
| _version_ | 1818552149569699840 |
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| author | Boussaid M. Naghmouchi S. Khouja M.L. Rejeb M.N. |
| author_facet | Boussaid M. Naghmouchi S. Khouja M.L. Rejeb M.N. |
| author_sort | Boussaid M. |
| collection | DOAJ |
| description | The present work was undertaken to develop a basic and simple protocol for micropropagation of Ceratonia siliqua. Axillaries bud sprouting and shoot development were stimulated on MS supplemented with BAP (0.5 mg.l-1), IBA (0.1 mg.l-1) and GA3 (0.5 mg.l-1), shoot multiplication was obtained on MS supplemented with BAP (2 mg.l-1) and rooting of microshoots was achieved on MS supplemented with IBA (2 mg.l-1) and charcoal (2 mg.l-1). The best results were obtained with herbaceous explants taken from juvenile trees. Significant differences in proliferation, multiplication and rooting due to the type and origin of explant and to the concentration of growth regulators were found. |
| first_indexed | 2024-12-12T09:09:25Z |
| format | Article |
| id | doaj.art-da709fab64ea484891df37e7ad4d5f82 |
| institution | Directory Open Access Journal |
| issn | 1370-6233 1780-4507 |
| language | English |
| last_indexed | 2024-12-12T09:09:25Z |
| publishDate | 2008-01-01 |
| publisher | Presses Agronomiques de Gembloux |
| record_format | Article |
| series | Biotechnologie, Agronomie, Société et Environnement |
| spelling | doaj.art-da709fab64ea484891df37e7ad4d5f822022-12-22T00:29:34ZengPresses Agronomiques de GemblouxBiotechnologie, Agronomie, Société et Environnement1370-62331780-45072008-01-01123251258Effect of growth regulators and explant origin on in vitro propagation of Ceratonia siliqua L. via cuttingsBoussaid M.Naghmouchi S.Khouja M.L.Rejeb M.N.The present work was undertaken to develop a basic and simple protocol for micropropagation of Ceratonia siliqua. Axillaries bud sprouting and shoot development were stimulated on MS supplemented with BAP (0.5 mg.l-1), IBA (0.1 mg.l-1) and GA3 (0.5 mg.l-1), shoot multiplication was obtained on MS supplemented with BAP (2 mg.l-1) and rooting of microshoots was achieved on MS supplemented with IBA (2 mg.l-1) and charcoal (2 mg.l-1). The best results were obtained with herbaceous explants taken from juvenile trees. Significant differences in proliferation, multiplication and rooting due to the type and origin of explant and to the concentration of growth regulators were found.http://www.pressesagro.be/base/text/v12n3/251.pdfbud sproutingrooting |
| spellingShingle | Boussaid M. Naghmouchi S. Khouja M.L. Rejeb M.N. Effect of growth regulators and explant origin on in vitro propagation of Ceratonia siliqua L. via cuttings Biotechnologie, Agronomie, Société et Environnement bud sprouting rooting |
| title | Effect of growth regulators and explant origin on in vitro propagation of Ceratonia siliqua L. via cuttings |
| title_full | Effect of growth regulators and explant origin on in vitro propagation of Ceratonia siliqua L. via cuttings |
| title_fullStr | Effect of growth regulators and explant origin on in vitro propagation of Ceratonia siliqua L. via cuttings |
| title_full_unstemmed | Effect of growth regulators and explant origin on in vitro propagation of Ceratonia siliqua L. via cuttings |
| title_short | Effect of growth regulators and explant origin on in vitro propagation of Ceratonia siliqua L. via cuttings |
| title_sort | effect of growth regulators and explant origin on in vitro propagation of ceratonia siliqua l via cuttings |
| topic | bud sprouting rooting |
| url | http://www.pressesagro.be/base/text/v12n3/251.pdf |
| work_keys_str_mv | AT boussaidm effectofgrowthregulatorsandexplantoriginoninvitropropagationofceratoniasiliqualviacuttings AT naghmouchis effectofgrowthregulatorsandexplantoriginoninvitropropagationofceratoniasiliqualviacuttings AT khoujaml effectofgrowthregulatorsandexplantoriginoninvitropropagationofceratoniasiliqualviacuttings AT rejebmn effectofgrowthregulatorsandexplantoriginoninvitropropagationofceratoniasiliqualviacuttings |