Transcriptomics and Metabolomics Analysis Provides Insight into Leaf Color and Photosynthesis Variation of the Yellow-Green Leaf Mutant of Hami Melon (<i>Cucumis melo</i> L.)
Leaf color mutants are ideal materials for studying the regulatory mechanism of chloroplast development and photosynthesis. We isolated a cucumis melo spontaneous mutant (MT), which showed yellow-green leaf phenotype in the whole growing period and could be inherited stably. We compared its leaves w...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/8/1623 |
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| author | Hongwei Han Yuan Zhou Huifang Liu Xianjun Chen Qiang Wang Hongmei Zhuang Xiaoxia Sun Qihua Ling Huijun Zhang Baike Wang Juan Wang Yaping Tang Hao Wang Huiying Liu |
| author_facet | Hongwei Han Yuan Zhou Huifang Liu Xianjun Chen Qiang Wang Hongmei Zhuang Xiaoxia Sun Qihua Ling Huijun Zhang Baike Wang Juan Wang Yaping Tang Hao Wang Huiying Liu |
| author_sort | Hongwei Han |
| collection | DOAJ |
| description | Leaf color mutants are ideal materials for studying the regulatory mechanism of chloroplast development and photosynthesis. We isolated a cucumis melo spontaneous mutant (MT), which showed yellow-green leaf phenotype in the whole growing period and could be inherited stably. We compared its leaves with the wild type (WT) in terms of cytology, physiology, transcriptome and metabolism. The results showed that the thylakoid grana lamellae of MT were loosely arranged and fewer in number than WT. Physiological experiments also showed that MT had less chlorophyll content and more accumulation of reactive oxygen species (ROS) than WT. Furthermore, the activity of several key enzymes in C<sub>4</sub> photosynthetic carbon assimilation pathway was more enhanced in MT than WT. Transcriptomic and metabolomic analyses showed that differential expression genes and differentially accumulated metabolites in MT were mainly co-enriched in the pathways related to photosystem-antenna proteins, central carbon metabolism, glutathione metabolism, phenylpropanoid biosynthesis and flavonoid metabolism. We also analyzed several key proteins in photosynthesis and chloroplast transport by Western blot. In summary, the results may provide a new insight into the understanding of how plants respond to the impaired photosynthesis by regulating chloroplast development and photosynthetic carbon assimilation pathways. |
| first_indexed | 2024-03-11T04:37:44Z |
| format | Article |
| id | doaj.art-da72bbba46eb40478d94779024d29663 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T04:37:44Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-da72bbba46eb40478d94779024d296632023-11-17T20:59:19ZengMDPI AGPlants2223-77472023-04-01128162310.3390/plants12081623Transcriptomics and Metabolomics Analysis Provides Insight into Leaf Color and Photosynthesis Variation of the Yellow-Green Leaf Mutant of Hami Melon (<i>Cucumis melo</i> L.)Hongwei Han0Yuan Zhou1Huifang Liu2Xianjun Chen3Qiang Wang4Hongmei Zhuang5Xiaoxia Sun6Qihua Ling7Huijun Zhang8Baike Wang9Juan Wang10Yaping Tang11Hao Wang12Huiying Liu13Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization of Xinjiang Production and Construction Corps, Department of Horticulture, College of Agriculture, Shihezi University, Shihezi 832003, ChinaNational Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200030, ChinaKey Laboratory of Horticulture Crop Genomics and Genetic Improvement in Xinjiang, Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830002, ChinaKey Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization of Xinjiang Production and Construction Corps, Department of Horticulture, College of Agriculture, Shihezi University, Shihezi 832003, ChinaKey Laboratory of Horticulture Crop Genomics and Genetic Improvement in Xinjiang, Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830002, ChinaKey Laboratory of Horticulture Crop Genomics and Genetic Improvement in Xinjiang, Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830002, ChinaKey Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization of Xinjiang Production and Construction Corps, Department of Horticulture, College of Agriculture, Shihezi University, Shihezi 832003, ChinaNational Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200030, ChinaSchool of Life Science, Huaibei Normal University, Huaibei 235000, ChinaKey Laboratory of Horticulture Crop Genomics and Genetic Improvement in Xinjiang, Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830002, ChinaKey Laboratory of Horticulture Crop Genomics and Genetic Improvement in Xinjiang, Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830002, ChinaKey Laboratory of Horticulture Crop Genomics and Genetic Improvement in Xinjiang, Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830002, ChinaKey Laboratory of Horticulture Crop Genomics and Genetic Improvement in Xinjiang, Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830002, ChinaKey Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization of Xinjiang Production and Construction Corps, Department of Horticulture, College of Agriculture, Shihezi University, Shihezi 832003, ChinaLeaf color mutants are ideal materials for studying the regulatory mechanism of chloroplast development and photosynthesis. We isolated a cucumis melo spontaneous mutant (MT), which showed yellow-green leaf phenotype in the whole growing period and could be inherited stably. We compared its leaves with the wild type (WT) in terms of cytology, physiology, transcriptome and metabolism. The results showed that the thylakoid grana lamellae of MT were loosely arranged and fewer in number than WT. Physiological experiments also showed that MT had less chlorophyll content and more accumulation of reactive oxygen species (ROS) than WT. Furthermore, the activity of several key enzymes in C<sub>4</sub> photosynthetic carbon assimilation pathway was more enhanced in MT than WT. Transcriptomic and metabolomic analyses showed that differential expression genes and differentially accumulated metabolites in MT were mainly co-enriched in the pathways related to photosystem-antenna proteins, central carbon metabolism, glutathione metabolism, phenylpropanoid biosynthesis and flavonoid metabolism. We also analyzed several key proteins in photosynthesis and chloroplast transport by Western blot. In summary, the results may provide a new insight into the understanding of how plants respond to the impaired photosynthesis by regulating chloroplast development and photosynthetic carbon assimilation pathways.https://www.mdpi.com/2223-7747/12/8/1623Hami melon mutantchloroplastphotosynthesisphotosynthetic carbon assimilationROStranscriptome |
| spellingShingle | Hongwei Han Yuan Zhou Huifang Liu Xianjun Chen Qiang Wang Hongmei Zhuang Xiaoxia Sun Qihua Ling Huijun Zhang Baike Wang Juan Wang Yaping Tang Hao Wang Huiying Liu Transcriptomics and Metabolomics Analysis Provides Insight into Leaf Color and Photosynthesis Variation of the Yellow-Green Leaf Mutant of Hami Melon (<i>Cucumis melo</i> L.) Plants Hami melon mutant chloroplast photosynthesis photosynthetic carbon assimilation ROS transcriptome |
| title | Transcriptomics and Metabolomics Analysis Provides Insight into Leaf Color and Photosynthesis Variation of the Yellow-Green Leaf Mutant of Hami Melon (<i>Cucumis melo</i> L.) |
| title_full | Transcriptomics and Metabolomics Analysis Provides Insight into Leaf Color and Photosynthesis Variation of the Yellow-Green Leaf Mutant of Hami Melon (<i>Cucumis melo</i> L.) |
| title_fullStr | Transcriptomics and Metabolomics Analysis Provides Insight into Leaf Color and Photosynthesis Variation of the Yellow-Green Leaf Mutant of Hami Melon (<i>Cucumis melo</i> L.) |
| title_full_unstemmed | Transcriptomics and Metabolomics Analysis Provides Insight into Leaf Color and Photosynthesis Variation of the Yellow-Green Leaf Mutant of Hami Melon (<i>Cucumis melo</i> L.) |
| title_short | Transcriptomics and Metabolomics Analysis Provides Insight into Leaf Color and Photosynthesis Variation of the Yellow-Green Leaf Mutant of Hami Melon (<i>Cucumis melo</i> L.) |
| title_sort | transcriptomics and metabolomics analysis provides insight into leaf color and photosynthesis variation of the yellow green leaf mutant of hami melon i cucumis melo i l |
| topic | Hami melon mutant chloroplast photosynthesis photosynthetic carbon assimilation ROS transcriptome |
| url | https://www.mdpi.com/2223-7747/12/8/1623 |
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