Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia
<p>Abstract</p> <p>The descriptive distribution and phylogeny of feline coronaviruses (FCoVs) were studied in cats suspected of having feline infectious peritonitis (FIP) in Malaysia. Ascitic fluids and/or biopsy samples were subjected to a reverse transcription polymerase chain re...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-01-01
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| Series: | Acta Veterinaria Scandinavica |
| Online Access: | http://www.actavetscand.com/content/52/1/1 |
| _version_ | 1811283288352882688 |
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| author | Arshad Habibah Rahman Nor-Alimah Fong Lau S Zeenathul Nazariah A Omar Abdul R Hair-Bejo Mohd Arshad Siti S Sharif Saeed Shamsudin Shahirudin Isa Mohd-Kamarudin A |
| author_facet | Arshad Habibah Rahman Nor-Alimah Fong Lau S Zeenathul Nazariah A Omar Abdul R Hair-Bejo Mohd Arshad Siti S Sharif Saeed Shamsudin Shahirudin Isa Mohd-Kamarudin A |
| author_sort | Arshad Habibah |
| collection | DOAJ |
| description | <p>Abstract</p> <p>The descriptive distribution and phylogeny of feline coronaviruses (FCoVs) were studied in cats suspected of having feline infectious peritonitis (FIP) in Malaysia. Ascitic fluids and/or biopsy samples were subjected to a reverse transcription polymerase chain reaction (RT-PCR) targeted for a conserved region of 3'untranslated region (3'UTR) of the FCoV genome. Eighty nine percent of the sampled animals were positive for the presence of FCoV. Among the FCoV positive cats, 80% of cats were males and 64% were below 2 years of age. The FCoV positive cases included 56% domestic short hair (DSH), 40% Persian, and 4% Siamese cats. The nucleotide sequences of 10 selected amplified products from FIP cases were determined. The sequence comparison revealed that the field isolates had 96% homology with a few point mutations. The extent of homology decreased to 93% when compared with reference strains. The overall branching pattern of phylogenetic tree showed two distinct clusters, where all Malaysian isolates fall into one main genetic cluster. These findings provided the first genetic information of FCoV in Malaysia.</p> |
| first_indexed | 2024-04-13T02:08:44Z |
| format | Article |
| id | doaj.art-da736fa2fbe648259c598f238bce22a9 |
| institution | Directory Open Access Journal |
| issn | 1751-0147 |
| language | English |
| last_indexed | 2024-04-13T02:08:44Z |
| publishDate | 2010-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Veterinaria Scandinavica |
| spelling | doaj.art-da736fa2fbe648259c598f238bce22a92022-12-22T03:07:22ZengBMCActa Veterinaria Scandinavica1751-01472010-01-01521110.1186/1751-0147-52-1Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of MalaysiaArshad HabibahRahman Nor-AlimahFong Lau SZeenathul Nazariah AOmar Abdul RHair-Bejo MohdArshad Siti SSharif SaeedShamsudin ShahirudinIsa Mohd-Kamarudin A<p>Abstract</p> <p>The descriptive distribution and phylogeny of feline coronaviruses (FCoVs) were studied in cats suspected of having feline infectious peritonitis (FIP) in Malaysia. Ascitic fluids and/or biopsy samples were subjected to a reverse transcription polymerase chain reaction (RT-PCR) targeted for a conserved region of 3'untranslated region (3'UTR) of the FCoV genome. Eighty nine percent of the sampled animals were positive for the presence of FCoV. Among the FCoV positive cats, 80% of cats were males and 64% were below 2 years of age. The FCoV positive cases included 56% domestic short hair (DSH), 40% Persian, and 4% Siamese cats. The nucleotide sequences of 10 selected amplified products from FIP cases were determined. The sequence comparison revealed that the field isolates had 96% homology with a few point mutations. The extent of homology decreased to 93% when compared with reference strains. The overall branching pattern of phylogenetic tree showed two distinct clusters, where all Malaysian isolates fall into one main genetic cluster. These findings provided the first genetic information of FCoV in Malaysia.</p>http://www.actavetscand.com/content/52/1/1 |
| spellingShingle | Arshad Habibah Rahman Nor-Alimah Fong Lau S Zeenathul Nazariah A Omar Abdul R Hair-Bejo Mohd Arshad Siti S Sharif Saeed Shamsudin Shahirudin Isa Mohd-Kamarudin A Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia Acta Veterinaria Scandinavica |
| title | Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia |
| title_full | Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia |
| title_fullStr | Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia |
| title_full_unstemmed | Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia |
| title_short | Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia |
| title_sort | descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of malaysia |
| url | http://www.actavetscand.com/content/52/1/1 |
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