Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1
Abstract Background Reovirus type-3 infections cause severe pathologies in young mice and thus influence animal experiments in many ways. Therefore, the Federation of Laboratory Animal Science Associations (FELASA) recommends an annual screening in laboratory mice as part of a thorough health monito...
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| Format: | Article |
| Language: | English |
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BMC
2018-07-01
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| Series: | Virology Journal |
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| Online Access: | http://link.springer.com/article/10.1186/s12985-018-1021-8 |
| _version_ | 1828529798149832704 |
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| author | Felix Fingas Daniela Volke Petra Bielefeldt Rayk Hassert Ralf Hoffmann |
| author_facet | Felix Fingas Daniela Volke Petra Bielefeldt Rayk Hassert Ralf Hoffmann |
| author_sort | Felix Fingas |
| collection | DOAJ |
| description | Abstract Background Reovirus type-3 infections cause severe pathologies in young mice and thus influence animal experiments in many ways. Therefore, the Federation of Laboratory Animal Science Associations (FELASA) recommends an annual screening in laboratory mice as part of a thorough health monitoring program. Based on the high protein sequence homology among the different reovirus serotypes, immunofluorescence antibody assay and other indirect methods relying on the whole virus are presumably cross-reactive to antibodies triggered by mammalian orthoreovirus infections independent of the serotype. Methods The serotype-specific protein σ-1 was expressed in Escherichia coli with an N-terminal Strep-tag and a C-terminal His-tag. The purified Strep-rσ-1-His-construct was used to develop an indirect ELISA by testing defined positive and negative sera obtained by experimental infection of mice as well as field sera. Results The Strep-rσ-1-His-ELISA provided high sensitivity and specificity during validation. Notably, a high selectivity was also observed for sera positively tested for other relevant FELASA-listed pathogens. Screening of field samples indicated that a commercial reovirus type-3-based ELISA might be cross-reactive to other murine reovirus serotypes and thus produces false-positive results. Conclusions The prevalence of reovirus type-3 might be overestimated in German animal facilities and most likely in other countries as well. The occurrence of other reovirus serotypes, however, raises the question if murine health monitoring programs should be extended to these pathogens. |
| first_indexed | 2024-12-11T22:14:04Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-11T22:14:04Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-da7718726255466ab81a921461ca1bcc2022-12-22T00:48:40ZengBMCVirology Journal1743-422X2018-07-0115111110.1186/s12985-018-1021-8Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1Felix Fingas0Daniela Volke1Petra Bielefeldt2Rayk Hassert3Ralf Hoffmann4Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität LeipzigInstitute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität LeipzigGVG Diagnostics GmbHInstitute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität LeipzigInstitute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität LeipzigAbstract Background Reovirus type-3 infections cause severe pathologies in young mice and thus influence animal experiments in many ways. Therefore, the Federation of Laboratory Animal Science Associations (FELASA) recommends an annual screening in laboratory mice as part of a thorough health monitoring program. Based on the high protein sequence homology among the different reovirus serotypes, immunofluorescence antibody assay and other indirect methods relying on the whole virus are presumably cross-reactive to antibodies triggered by mammalian orthoreovirus infections independent of the serotype. Methods The serotype-specific protein σ-1 was expressed in Escherichia coli with an N-terminal Strep-tag and a C-terminal His-tag. The purified Strep-rσ-1-His-construct was used to develop an indirect ELISA by testing defined positive and negative sera obtained by experimental infection of mice as well as field sera. Results The Strep-rσ-1-His-ELISA provided high sensitivity and specificity during validation. Notably, a high selectivity was also observed for sera positively tested for other relevant FELASA-listed pathogens. Screening of field samples indicated that a commercial reovirus type-3-based ELISA might be cross-reactive to other murine reovirus serotypes and thus produces false-positive results. Conclusions The prevalence of reovirus type-3 might be overestimated in German animal facilities and most likely in other countries as well. The occurrence of other reovirus serotypes, however, raises the question if murine health monitoring programs should be extended to these pathogens.http://link.springer.com/article/10.1186/s12985-018-1021-8Mammalian orthoreovirus type-3FELASAELISA |
| spellingShingle | Felix Fingas Daniela Volke Petra Bielefeldt Rayk Hassert Ralf Hoffmann Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1 Virology Journal Mammalian orthoreovirus type-3 FELASA ELISA |
| title | Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1 |
| title_full | Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1 |
| title_fullStr | Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1 |
| title_full_unstemmed | Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1 |
| title_short | Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1 |
| title_sort | detection of mammalian orthoreovirus type 3 reo 3 infections in mice based on serotype specific hemagglutination protein sigma 1 |
| topic | Mammalian orthoreovirus type-3 FELASA ELISA |
| url | http://link.springer.com/article/10.1186/s12985-018-1021-8 |
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