Investigation of allelic frequency and forensic genetics parameter for 10 STR loci in Arab and Kurd ethnics of Iran
Background and Aim: Short tandem repeat (STR) markers, are conserved region in human genome and highly polymorphic between individuals. Nowadays, genotyping of STR marker is widely known and used for the genetic identification of individuals in forensic DNA analyses. Based on allelic frequencies of...
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| Format: | Article |
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Birjand University of Medical Sciences and Health Services
2018-03-01
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| Series: | مجله دانشگاه علوم پزشکی بیرجند |
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| Online Access: | http://journal.bums.ac.ir/browse.php?a_code=A-10-2208-1&slc_lang=en&sid=1 |
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| author | Mohammadreza Nassiri Shahrokh Ghovvati Seyed Ziaedin Mirhoseini Ali Javadmanesh Morteza Mahdavi Arash Alipour Masoume Vakili-Azghandi |
| author_facet | Mohammadreza Nassiri Shahrokh Ghovvati Seyed Ziaedin Mirhoseini Ali Javadmanesh Morteza Mahdavi Arash Alipour Masoume Vakili-Azghandi |
| author_sort | Mohammadreza Nassiri |
| collection | DOAJ |
| description | Background and Aim: Short tandem repeat (STR) markers, are conserved region in human genome and highly polymorphic between individuals. Nowadays, genotyping of STR marker is widely known and used for the genetic identification of individuals in forensic DNA analyses. Based on allelic frequencies of STR loci varies between populations, investigation of genetically and forensically parameters in each population and characterization of these markers is necessary. The objective of this study was to optimizing laboratory method for application of 10autosomal STR loci (TPOX، vWA، D7S820، D8S1179، D13S317، D16S539، D18S51، D5S818، THO1، D21S311) in Kurd and Arab ethnics of Iran and investigation of population and forensic genetics parameter of these markers in these populations.
Materials and Methods: In this semi-experimental study, blood samples from 93 Arab and 94 Kurd individuals were collected. After DNA extraction, PCR amplification was carried out for 10 autosomal STR loci, individually. Then, acrylamide gel electrophoresis was used to determine the genotype of each individual in each site.
Results: Deviation from Hardy Weinberg equilibrium even after Bonfferroni correction was seen in the locus of D13S317in both Populations. After D13S317 loci, the highest observed heterozygosity was seen in D21S311loci for Kurd population (94%) and in THO1, vWA and D5S818 locifor Arab population (84%). The lowest observed heterozygosity (0.71 and 0.72) was seen in TPOX loci for both populations form Arab and Kurd ethnics, respectively. Investigation of forensic genetic parameters (PI, PE, PD, and PIC) showed that in except of the D13S317 loci other remaining evaluated locus had proper properties for using in genetics fingertips in both of Kourd and Arab ethnics.
Conclusion: The results of current study indicate that the necessity investigation of forensic genetics for rapid characterization of the different ethnicities which located in different geographic parts of Iran in order to choose the appropriate data set to calculate of forensic genetics parameters not only within each ethnic but also between them.
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| first_indexed | 2024-12-12T12:23:14Z |
| format | Article |
| id | doaj.art-da785c8e47c74884a4e70412bffbd03f |
| institution | Directory Open Access Journal |
| issn | 1607-2197 2423-6152 |
| language | fas |
| last_indexed | 2024-12-12T12:23:14Z |
| publishDate | 2018-03-01 |
| publisher | Birjand University of Medical Sciences and Health Services |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی بیرجند |
| spelling | doaj.art-da785c8e47c74884a4e70412bffbd03f2022-12-22T00:24:37ZfasBirjand University of Medical Sciences and Health Servicesمجله دانشگاه علوم پزشکی بیرجند1607-21972423-61522018-03-012513141Investigation of allelic frequency and forensic genetics parameter for 10 STR loci in Arab and Kurd ethnics of IranMohammadreza Nassiri0Shahrokh Ghovvati1Seyed Ziaedin Mirhoseini2Ali Javadmanesh3Morteza Mahdavi4Arash Alipour5Masoume Vakili-Azghandi6 Recombinant ProteinsResearch Group, The Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran Genetics Subgroup, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran Recombinant Proteins Research Group, The Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran Genetics Subgroup, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. General Office of Legal Medicine, Razavi Khorasan, Mashhad, Iran Genetics Subgroup, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Background and Aim: Short tandem repeat (STR) markers, are conserved region in human genome and highly polymorphic between individuals. Nowadays, genotyping of STR marker is widely known and used for the genetic identification of individuals in forensic DNA analyses. Based on allelic frequencies of STR loci varies between populations, investigation of genetically and forensically parameters in each population and characterization of these markers is necessary. The objective of this study was to optimizing laboratory method for application of 10autosomal STR loci (TPOX، vWA، D7S820، D8S1179، D13S317، D16S539، D18S51، D5S818، THO1، D21S311) in Kurd and Arab ethnics of Iran and investigation of population and forensic genetics parameter of these markers in these populations. Materials and Methods: In this semi-experimental study, blood samples from 93 Arab and 94 Kurd individuals were collected. After DNA extraction, PCR amplification was carried out for 10 autosomal STR loci, individually. Then, acrylamide gel electrophoresis was used to determine the genotype of each individual in each site. Results: Deviation from Hardy Weinberg equilibrium even after Bonfferroni correction was seen in the locus of D13S317in both Populations. After D13S317 loci, the highest observed heterozygosity was seen in D21S311loci for Kurd population (94%) and in THO1, vWA and D5S818 locifor Arab population (84%). The lowest observed heterozygosity (0.71 and 0.72) was seen in TPOX loci for both populations form Arab and Kurd ethnics, respectively. Investigation of forensic genetic parameters (PI, PE, PD, and PIC) showed that in except of the D13S317 loci other remaining evaluated locus had proper properties for using in genetics fingertips in both of Kourd and Arab ethnics. Conclusion: The results of current study indicate that the necessity investigation of forensic genetics for rapid characterization of the different ethnicities which located in different geographic parts of Iran in order to choose the appropriate data set to calculate of forensic genetics parameters not only within each ethnic but also between them. http://journal.bums.ac.ir/browse.php?a_code=A-10-2208-1&slc_lang=en&sid=1DNA Fingerprinting Allelic frequencies Identity determination STR Marker Arab and Kurd ethnics Forensic genetics |
| spellingShingle | Mohammadreza Nassiri Shahrokh Ghovvati Seyed Ziaedin Mirhoseini Ali Javadmanesh Morteza Mahdavi Arash Alipour Masoume Vakili-Azghandi Investigation of allelic frequency and forensic genetics parameter for 10 STR loci in Arab and Kurd ethnics of Iran مجله دانشگاه علوم پزشکی بیرجند DNA Fingerprinting Allelic frequencies Identity determination STR Marker Arab and Kurd ethnics Forensic genetics |
| title | Investigation of allelic frequency and forensic genetics parameter for 10 STR loci in Arab and Kurd ethnics of Iran |
| title_full | Investigation of allelic frequency and forensic genetics parameter for 10 STR loci in Arab and Kurd ethnics of Iran |
| title_fullStr | Investigation of allelic frequency and forensic genetics parameter for 10 STR loci in Arab and Kurd ethnics of Iran |
| title_full_unstemmed | Investigation of allelic frequency and forensic genetics parameter for 10 STR loci in Arab and Kurd ethnics of Iran |
| title_short | Investigation of allelic frequency and forensic genetics parameter for 10 STR loci in Arab and Kurd ethnics of Iran |
| title_sort | investigation of allelic frequency and forensic genetics parameter for 10 str loci in arab and kurd ethnics of iran |
| topic | DNA Fingerprinting Allelic frequencies Identity determination STR Marker Arab and Kurd ethnics Forensic genetics |
| url | http://journal.bums.ac.ir/browse.php?a_code=A-10-2208-1&slc_lang=en&sid=1 |
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