Genome-Wide Identification and Expression Profile Reveal Potential Roles of Peanut <i>ZIP</i> Family Genes in Zinc/Iron-Deficiency Tolerance
Zinc/iron-regulated transporter-like protein (ZIP) family genes play crucial roles in metal uptake and transport in plants. However, little is known about their functions in peanut. Here, genome-wide analysis identified 30 peanut <i>AhZIP</i> genes that were divided into four classes. Mo...
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MDPI AG
2022-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/6/786 |
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| author | Zhen Zhang Nannan Chen Zheng Zhang Gangrong Shi |
| author_facet | Zhen Zhang Nannan Chen Zheng Zhang Gangrong Shi |
| author_sort | Zhen Zhang |
| collection | DOAJ |
| description | Zinc/iron-regulated transporter-like protein (ZIP) family genes play crucial roles in metal uptake and transport in plants. However, little is known about their functions in peanut. Here, genome-wide analysis identified 30 peanut <i>AhZIP</i> genes that were divided into four classes. Most <i>AhZIP</i>s experienced whole-genome or segmental duplication. AhZIP proteins harbored 3–8 transmembrane domains and a typical ZIP domain, showing considerable homology with BbZIP from <i>Bordetella bronchiseptica</i>. Clustered <i>AhZIP</i>s generally share similar gene/protein structures; however, unique features were found in <i>AhIRT1.2</i>, <i>AhZIP1.2</i>, <i>AhZIP3.5</i> and <i>AhZIP7.8</i>. RNA-seq data revealed that <i>AhZIP2.1</i>/<i>2.2</i>, <i>AhZIP4.1</i>/<i>4.2</i> and <i>AhZIP11.1</i>/<i>11.2</i> were highly and preferentially expressed in roots, nodule and reproductive tissues. RT-qPCR analysis indicated that transcriptional responses of <i>AhZIP</i>s to Fe/Zn deficiency are cultivar dependent. The expressions of <i>AhIRT1.1</i>, <i>AhIRT1.2</i> and <i>AhZIP6.1</i> were closely related to Fe uptake and translocation. <i>AhIRT1.1</i> and <i>AhZIP7.2</i> expression were significantly correlated with Zn accumulation. The expression of <i>AhIRT1.1</i>, <i>AhIRT1.2</i>, <i>AhZIP3.6</i>, <i>AhZIP6.1</i> and <i>AhZIP11.1</i> was associated with Mn uptake and translocation. The results confirmed that <i>AhZIP</i> genes play crucial roles in the uptake and transport of Fe, Zn and Mn in peanut, providing clues to further functionally characterize <i>AhZIP</i> genes in the future. |
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| language | English |
| last_indexed | 2024-03-09T12:55:20Z |
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| spelling | doaj.art-da792c3cdb7d41ed9e43ab1bcb1216d82023-11-30T22:01:03ZengMDPI AGPlants2223-77472022-03-0111678610.3390/plants11060786Genome-Wide Identification and Expression Profile Reveal Potential Roles of Peanut <i>ZIP</i> Family Genes in Zinc/Iron-Deficiency ToleranceZhen Zhang0Nannan Chen1Zheng Zhang2Gangrong Shi3College of Life Sciences, Huaibei Normal University, Huaibei 235000, ChinaCollege of Life Sciences, Huaibei Normal University, Huaibei 235000, ChinaCollege of Life Sciences, Huaibei Normal University, Huaibei 235000, ChinaCollege of Life Sciences, Huaibei Normal University, Huaibei 235000, ChinaZinc/iron-regulated transporter-like protein (ZIP) family genes play crucial roles in metal uptake and transport in plants. However, little is known about their functions in peanut. Here, genome-wide analysis identified 30 peanut <i>AhZIP</i> genes that were divided into four classes. Most <i>AhZIP</i>s experienced whole-genome or segmental duplication. AhZIP proteins harbored 3–8 transmembrane domains and a typical ZIP domain, showing considerable homology with BbZIP from <i>Bordetella bronchiseptica</i>. Clustered <i>AhZIP</i>s generally share similar gene/protein structures; however, unique features were found in <i>AhIRT1.2</i>, <i>AhZIP1.2</i>, <i>AhZIP3.5</i> and <i>AhZIP7.8</i>. RNA-seq data revealed that <i>AhZIP2.1</i>/<i>2.2</i>, <i>AhZIP4.1</i>/<i>4.2</i> and <i>AhZIP11.1</i>/<i>11.2</i> were highly and preferentially expressed in roots, nodule and reproductive tissues. RT-qPCR analysis indicated that transcriptional responses of <i>AhZIP</i>s to Fe/Zn deficiency are cultivar dependent. The expressions of <i>AhIRT1.1</i>, <i>AhIRT1.2</i> and <i>AhZIP6.1</i> were closely related to Fe uptake and translocation. <i>AhIRT1.1</i> and <i>AhZIP7.2</i> expression were significantly correlated with Zn accumulation. The expression of <i>AhIRT1.1</i>, <i>AhIRT1.2</i>, <i>AhZIP3.6</i>, <i>AhZIP6.1</i> and <i>AhZIP11.1</i> was associated with Mn uptake and translocation. The results confirmed that <i>AhZIP</i> genes play crucial roles in the uptake and transport of Fe, Zn and Mn in peanut, providing clues to further functionally characterize <i>AhZIP</i> genes in the future.https://www.mdpi.com/2223-7747/11/6/786<i>Arachis hypogaea</i>ZRT/IRT-like proteiniron/zinc deficiencymetal uptake and transportcultivar difference |
| spellingShingle | Zhen Zhang Nannan Chen Zheng Zhang Gangrong Shi Genome-Wide Identification and Expression Profile Reveal Potential Roles of Peanut <i>ZIP</i> Family Genes in Zinc/Iron-Deficiency Tolerance Plants <i>Arachis hypogaea</i> ZRT/IRT-like protein iron/zinc deficiency metal uptake and transport cultivar difference |
| title | Genome-Wide Identification and Expression Profile Reveal Potential Roles of Peanut <i>ZIP</i> Family Genes in Zinc/Iron-Deficiency Tolerance |
| title_full | Genome-Wide Identification and Expression Profile Reveal Potential Roles of Peanut <i>ZIP</i> Family Genes in Zinc/Iron-Deficiency Tolerance |
| title_fullStr | Genome-Wide Identification and Expression Profile Reveal Potential Roles of Peanut <i>ZIP</i> Family Genes in Zinc/Iron-Deficiency Tolerance |
| title_full_unstemmed | Genome-Wide Identification and Expression Profile Reveal Potential Roles of Peanut <i>ZIP</i> Family Genes in Zinc/Iron-Deficiency Tolerance |
| title_short | Genome-Wide Identification and Expression Profile Reveal Potential Roles of Peanut <i>ZIP</i> Family Genes in Zinc/Iron-Deficiency Tolerance |
| title_sort | genome wide identification and expression profile reveal potential roles of peanut i zip i family genes in zinc iron deficiency tolerance |
| topic | <i>Arachis hypogaea</i> ZRT/IRT-like protein iron/zinc deficiency metal uptake and transport cultivar difference |
| url | https://www.mdpi.com/2223-7747/11/6/786 |
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