Development of Homozygous Lines by In Vivo Doubled Haploid Technique in Hybrid Maize Breeding
The maize breeding programs focuses on the development of homozygous parental lines for hybrid breeding to obtain heterosis. The Improvement of homozygous lines in a effective time is crucial for hybrid maize breeding. Objectives of this research were to obtain homozygous lines of maize in a short t...
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2023-08-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/6237 |
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| author | İbrahim Cerit Gönül Cömertpay Bülent Çakır Rüştü Hatipoğlu Hakan Özkan |
| author_facet | İbrahim Cerit Gönül Cömertpay Bülent Çakır Rüştü Hatipoğlu Hakan Özkan |
| author_sort | İbrahim Cerit |
| collection | DOAJ |
| description | The maize breeding programs focuses on the development of homozygous parental lines for hybrid breeding to obtain heterosis. The Improvement of homozygous lines in a effective time is crucial for hybrid maize breeding. Objectives of this research were to obtain homozygous lines of maize in a short time by using of in-vivo maternal haploid (DH) technique and characterizing them morphologically. The experiments were carried out at the Eastern Mediterranean Agricultural Research Institute(EMARI). Inducers, RWS, RWK-76 and their hybrid RWS X RWK-76 were used as male parent. As female parent, 56 F2 segregated material obtained by selfing of 66 F1 hybrids developed in maize breeding program of Eastern Mediterranean Agricultural Research Institute (EMARI) and 2 F2 segregated material selected from the open pollinated plants of 9 commercial maize variety. As a result of the experiment, 29 doubled haploid lines (DH) were developed. In the developed DH lines, days to tasseling, plant height, height of first ear , ear length, ear diameter, number of kernel per ear and thousand kernel weight varied as 57-78 days, 151-248 cm, 43-112 cm, 11.20-24.50 cm, 24-45 mm, 224-537 kernels/ear, and 180-320 g, respectively. In conclusion, in-vivo maternal DH technique is a highly effective method for obtaining homozygous lines |
| first_indexed | 2024-03-11T21:17:21Z |
| format | Article |
| id | doaj.art-da79f8ec57e841d4a523728115e050d8 |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-03-11T21:17:21Z |
| publishDate | 2023-08-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-da79f8ec57e841d4a523728115e050d82023-09-28T16:05:13ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2023-08-011181386139310.24925/turjaf.v11i8.1386-1393.62374938Development of Homozygous Lines by In Vivo Doubled Haploid Technique in Hybrid Maize Breedingİbrahim Cerit0https://orcid.org/0000-0001-6646-6653Gönül Cömertpay1https://orcid.org/0000-0002-6522-4596Bülent Çakır2https://orcid.org/0000-0003-4672-7582Rüştü Hatipoğlu3https://orcid.org/0000-0002-7977-0782Hakan Özkan4https://orcid.org/0000-0003-3530-2626Eastern Mediterranean Agricultural Research Institute, AdanaEastern Mediterranean Agricultural Research Institute, Adana, TürkiyeEastern Mediterranean Agricultural Research Institute, Adana, TürkiyeÇukurova University, Faculty of Agriculture, Department of Field Crops, Adana, TürkiyeÇukurova University, Faculty of Agriculture, Department of Field Crops, Adana, TürkiyeThe maize breeding programs focuses on the development of homozygous parental lines for hybrid breeding to obtain heterosis. The Improvement of homozygous lines in a effective time is crucial for hybrid maize breeding. Objectives of this research were to obtain homozygous lines of maize in a short time by using of in-vivo maternal haploid (DH) technique and characterizing them morphologically. The experiments were carried out at the Eastern Mediterranean Agricultural Research Institute(EMARI). Inducers, RWS, RWK-76 and their hybrid RWS X RWK-76 were used as male parent. As female parent, 56 F2 segregated material obtained by selfing of 66 F1 hybrids developed in maize breeding program of Eastern Mediterranean Agricultural Research Institute (EMARI) and 2 F2 segregated material selected from the open pollinated plants of 9 commercial maize variety. As a result of the experiment, 29 doubled haploid lines (DH) were developed. In the developed DH lines, days to tasseling, plant height, height of first ear , ear length, ear diameter, number of kernel per ear and thousand kernel weight varied as 57-78 days, 151-248 cm, 43-112 cm, 11.20-24.50 cm, 24-45 mm, 224-537 kernels/ear, and 180-320 g, respectively. In conclusion, in-vivo maternal DH technique is a highly effective method for obtaining homozygous lineshttp://www.agrifoodscience.com/index.php/TURJAF/article/view/6237double haploidhomozygous linesinducer linemaize |
| spellingShingle | İbrahim Cerit Gönül Cömertpay Bülent Çakır Rüştü Hatipoğlu Hakan Özkan Development of Homozygous Lines by In Vivo Doubled Haploid Technique in Hybrid Maize Breeding Turkish Journal of Agriculture: Food Science and Technology double haploid homozygous lines inducer line maize |
| title | Development of Homozygous Lines by In Vivo Doubled Haploid Technique in Hybrid Maize Breeding |
| title_full | Development of Homozygous Lines by In Vivo Doubled Haploid Technique in Hybrid Maize Breeding |
| title_fullStr | Development of Homozygous Lines by In Vivo Doubled Haploid Technique in Hybrid Maize Breeding |
| title_full_unstemmed | Development of Homozygous Lines by In Vivo Doubled Haploid Technique in Hybrid Maize Breeding |
| title_short | Development of Homozygous Lines by In Vivo Doubled Haploid Technique in Hybrid Maize Breeding |
| title_sort | development of homozygous lines by in vivo doubled haploid technique in hybrid maize breeding |
| topic | double haploid homozygous lines inducer line maize |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/6237 |
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