Incidence of fungus and physiological quality of seeds of Jatropha curcas L. after cryogenic storage

The objective of this work was to evaluate the incidence of fungi in stored seeds of Jatropha curcas. The research was carried out at Cryogenic/UFCG, Sanity/UFPB and Cotton/Embrapa. The material for analysis showed an 8% water level, and 200 seeds were stored for treatment in cryogenic containers wi...

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Main Authors: Míriam Goldfarb, Maria Elita Martins Duarte, Mário Eduardo R. M. Cavalcanti Mata, Luciana Cordeiro do Nascimento, Noelma Miranda de Brito, Francisca Maria Souto
Format: Article
Language:English
Published: Universidade Federal de Santa Catarina 2010-03-01
Series:Biotemas
Subjects:
Online Access:http://www.biotemas.ufsc.br/volumes/pdf/volume231/19a26.pdf
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author Míriam Goldfarb
Maria Elita Martins Duarte
Mário Eduardo R. M. Cavalcanti Mata
Luciana Cordeiro do Nascimento
Noelma Miranda de Brito
Francisca Maria Souto
author_facet Míriam Goldfarb
Maria Elita Martins Duarte
Mário Eduardo R. M. Cavalcanti Mata
Luciana Cordeiro do Nascimento
Noelma Miranda de Brito
Francisca Maria Souto
author_sort Míriam Goldfarb
collection DOAJ
description The objective of this work was to evaluate the incidence of fungi in stored seeds of Jatropha curcas. The research was carried out at Cryogenic/UFCG, Sanity/UFPB and Cotton/Embrapa. The material for analysis showed an 8% water level, and 200 seeds were stored for treatment in cryogenic containers with nitrogen in the vapor and liquid phases. Four periods of crioconservation (0, 30, 60 and 90 days), were employed. After each period, the seeds were tested for sanity (Blotter test) and germination. Superficial disinfestation, was carried out and seeds were distributed in Petri dishes, for incubation at 25 ± 2ºC, over a period of 7 days. The evaluation of the incidence of fungi was carried out in a stereoscopic microscope with observation of fungal structures, and values were expressed as percentages of seeds with fungus. The statistical experiment was completely randomized with temperature x days of storage. Analysis of variance was conducted and the means were compared by Tukey’s test at 5%. After 30 days of cryogenic storage, a greater incidence of Aspergillus sp., Cladosporium sp. and Fusarium sp. was detected. It was concluded that crioconservation at cryogenic temperatures did not reduce the incidence of fungus on Jatropha curcas seeds. The physiological quality was preserved during the cryoconservation.
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spelling doaj.art-da8020cdc24047e9a991bef212bfab352022-12-21T22:21:39ZengUniversidade Federal de Santa CatarinaBiotemas0103-16432010-03-012311926Incidence of fungus and physiological quality of seeds of Jatropha curcas L. after cryogenic storageMíriam GoldfarbMaria Elita Martins DuarteMário Eduardo R. M. Cavalcanti MataLuciana Cordeiro do NascimentoNoelma Miranda de BritoFrancisca Maria SoutoThe objective of this work was to evaluate the incidence of fungi in stored seeds of Jatropha curcas. The research was carried out at Cryogenic/UFCG, Sanity/UFPB and Cotton/Embrapa. The material for analysis showed an 8% water level, and 200 seeds were stored for treatment in cryogenic containers with nitrogen in the vapor and liquid phases. Four periods of crioconservation (0, 30, 60 and 90 days), were employed. After each period, the seeds were tested for sanity (Blotter test) and germination. Superficial disinfestation, was carried out and seeds were distributed in Petri dishes, for incubation at 25 ± 2ºC, over a period of 7 days. The evaluation of the incidence of fungi was carried out in a stereoscopic microscope with observation of fungal structures, and values were expressed as percentages of seeds with fungus. The statistical experiment was completely randomized with temperature x days of storage. Analysis of variance was conducted and the means were compared by Tukey’s test at 5%. After 30 days of cryogenic storage, a greater incidence of Aspergillus sp., Cladosporium sp. and Fusarium sp. was detected. It was concluded that crioconservation at cryogenic temperatures did not reduce the incidence of fungus on Jatropha curcas seeds. The physiological quality was preserved during the cryoconservation.http://www.biotemas.ufsc.br/volumes/pdf/volume231/19a26.pdfcryoconservationoil speciessanity and physiological analyses
spellingShingle Míriam Goldfarb
Maria Elita Martins Duarte
Mário Eduardo R. M. Cavalcanti Mata
Luciana Cordeiro do Nascimento
Noelma Miranda de Brito
Francisca Maria Souto
Incidence of fungus and physiological quality of seeds of Jatropha curcas L. after cryogenic storage
Biotemas
cryoconservation
oil species
sanity and physiological analyses
title Incidence of fungus and physiological quality of seeds of Jatropha curcas L. after cryogenic storage
title_full Incidence of fungus and physiological quality of seeds of Jatropha curcas L. after cryogenic storage
title_fullStr Incidence of fungus and physiological quality of seeds of Jatropha curcas L. after cryogenic storage
title_full_unstemmed Incidence of fungus and physiological quality of seeds of Jatropha curcas L. after cryogenic storage
title_short Incidence of fungus and physiological quality of seeds of Jatropha curcas L. after cryogenic storage
title_sort incidence of fungus and physiological quality of seeds of jatropha curcas l after cryogenic storage
topic cryoconservation
oil species
sanity and physiological analyses
url http://www.biotemas.ufsc.br/volumes/pdf/volume231/19a26.pdf
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