Regulation of squalene epoxidase activity and comparison of catalytic properties of rat liver and Chinese hamster ovary cell-derived enzymes.
Squalene epoxidase activity has been studied in cell-free preparations of Chinese hamster ovary (CHO) cells and rat liver. In contrast to rat liver microsomal squalene epoxidase, the enzyme of CHO cells is only slightly activated by the autologous cytosolic fraction, whereas phosphatidylglycerol or...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
1987-12-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520385783 |
| _version_ | 1819293704152678400 |
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| author | H Eilenberg I Shechter |
| author_facet | H Eilenberg I Shechter |
| author_sort | H Eilenberg |
| collection | DOAJ |
| description | Squalene epoxidase activity has been studied in cell-free preparations of Chinese hamster ovary (CHO) cells and rat liver. In contrast to rat liver microsomal squalene epoxidase, the enzyme of CHO cells is only slightly activated by the autologous cytosolic fraction, whereas phosphatidylglycerol or rat liver cytosolic preparations are potent stimulators of this enzyme. Triton X-100, a known stimulator of the hepatic squalene epoxidase, has no activating effect on the enzyme of CHO cells. The squalene epoxidase activity of both rat liver and CHO cells varies significantly according to the lipid content of the growth medium or diet. The changes in enzyme activity are shown to be entirely due to altered microsomal enzyme per se and not to changes in the activating properties of the soluble fraction. These results further support the proposed regulatory role of squalene epoxidase in cholesterogenesis. |
| first_indexed | 2024-12-24T04:14:39Z |
| format | Article |
| id | doaj.art-da80c1f9e844471394338085bca4edea |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-24T04:14:39Z |
| publishDate | 1987-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-da80c1f9e844471394338085bca4edea2022-12-21T17:15:58ZengElsevierJournal of Lipid Research0022-22751987-12-01281213981404Regulation of squalene epoxidase activity and comparison of catalytic properties of rat liver and Chinese hamster ovary cell-derived enzymes.H Eilenberg0I Shechter1Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.Squalene epoxidase activity has been studied in cell-free preparations of Chinese hamster ovary (CHO) cells and rat liver. In contrast to rat liver microsomal squalene epoxidase, the enzyme of CHO cells is only slightly activated by the autologous cytosolic fraction, whereas phosphatidylglycerol or rat liver cytosolic preparations are potent stimulators of this enzyme. Triton X-100, a known stimulator of the hepatic squalene epoxidase, has no activating effect on the enzyme of CHO cells. The squalene epoxidase activity of both rat liver and CHO cells varies significantly according to the lipid content of the growth medium or diet. The changes in enzyme activity are shown to be entirely due to altered microsomal enzyme per se and not to changes in the activating properties of the soluble fraction. These results further support the proposed regulatory role of squalene epoxidase in cholesterogenesis.http://www.sciencedirect.com/science/article/pii/S0022227520385783 |
| spellingShingle | H Eilenberg I Shechter Regulation of squalene epoxidase activity and comparison of catalytic properties of rat liver and Chinese hamster ovary cell-derived enzymes. Journal of Lipid Research |
| title | Regulation of squalene epoxidase activity and comparison of catalytic properties of rat liver and Chinese hamster ovary cell-derived enzymes. |
| title_full | Regulation of squalene epoxidase activity and comparison of catalytic properties of rat liver and Chinese hamster ovary cell-derived enzymes. |
| title_fullStr | Regulation of squalene epoxidase activity and comparison of catalytic properties of rat liver and Chinese hamster ovary cell-derived enzymes. |
| title_full_unstemmed | Regulation of squalene epoxidase activity and comparison of catalytic properties of rat liver and Chinese hamster ovary cell-derived enzymes. |
| title_short | Regulation of squalene epoxidase activity and comparison of catalytic properties of rat liver and Chinese hamster ovary cell-derived enzymes. |
| title_sort | regulation of squalene epoxidase activity and comparison of catalytic properties of rat liver and chinese hamster ovary cell derived enzymes |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520385783 |
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