Tooth-Specific <i>Streptococcus mutans</i> Distribution and Associated Microbiome
Dental caries is multifactorial and polymicrobial in nature and remains one of the most common oral diseases. While caries research has focused on <i>Streptococcus mutans</i> as the main etiological pathogen, its impact at the tooth level is not fully understood. In this cross-sectional...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/6/1129 |
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| author | Márcia Dinis William Traynor Melissa Agnello Myung-Shin Sim Xuesong He Wenyuan Shi Renate Lux Nini Chaichanasakul Tran |
| author_facet | Márcia Dinis William Traynor Melissa Agnello Myung-Shin Sim Xuesong He Wenyuan Shi Renate Lux Nini Chaichanasakul Tran |
| author_sort | Márcia Dinis |
| collection | DOAJ |
| description | Dental caries is multifactorial and polymicrobial in nature and remains one of the most common oral diseases. While caries research has focused on <i>Streptococcus mutans</i> as the main etiological pathogen, its impact at the tooth level is not fully understood. In this cross-sectional study, the levels and distribution of <i>S. mutans</i> in the posterior teeth at different dentition stages were investigated along with the corresponding tooth-specific microbiome. Occlusal plaque samples of 87 individual posterior teeth were collected from thirty children in three dentition stages (primary, mixed, and permanent). The <i>S. mutans</i> levels in the occlusal plaque of individual posterior teeth were quantified with qPCR, and those with preferential colonization were selected for tooth-specific microbiome analysis using 16S rRNA sequencing. Results: Quantification of <i>S. mutans</i> levels in the occlusal plaque confirmed the preferential colonization on the first primary and permanent molars. These teeth were selected for further tooth-specific microbiome sequencing, as they also displayed high caries experience. There were significant differences in the relative abundance of the four most abundant genera: <i>Neisseria</i>, <i>Streptococcus</i>, <i>Rothia,</i> and <i>Veillonella</i>. Furthermore, the tooth-level caries experience was correlated with a reduction in the microbiome diversity. Analyzing the different tooth-associated microbial communities, distinct tooth-specific core microbiomes were identified. Conclusions: Our findings suggest that caries susceptibility at the tooth level, depending on tooth type and dentition stage, is influenced by individual species as well as plaque community. |
| first_indexed | 2024-03-09T23:00:29Z |
| format | Article |
| id | doaj.art-da81488a13254076a4ffd7c473f7f570 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:00:29Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-da81488a13254076a4ffd7c473f7f5702023-11-23T18:03:29ZengMDPI AGMicroorganisms2076-26072022-05-01106112910.3390/microorganisms10061129Tooth-Specific <i>Streptococcus mutans</i> Distribution and Associated MicrobiomeMárcia Dinis0William Traynor1Melissa Agnello2Myung-Shin Sim3Xuesong He4Wenyuan Shi5Renate Lux6Nini Chaichanasakul Tran7Section of Pediatric Dentistry, School of Dentistry, University of California, Los Angeles, CA 90095, USASection of Pediatric Dentistry, School of Dentistry, University of California, Los Angeles, CA 90095, USASection of Oral Biology, School of Dentistry, University of California, Los Angeles, CA 90095, USADivision of General Internal Medicine and Health Services Research, Department of Medicine Statistics Core, University of California, Los Angeles, CA 90095, USASection of Oral Biology, School of Dentistry, University of California, Los Angeles, CA 90095, USASection of Oral Biology, School of Dentistry, University of California, Los Angeles, CA 90095, USASection of Periodontics, School of Dentistry, University of California, Los Angeles, CA 90095, USASection of Pediatric Dentistry, School of Dentistry, University of California, Los Angeles, CA 90095, USADental caries is multifactorial and polymicrobial in nature and remains one of the most common oral diseases. While caries research has focused on <i>Streptococcus mutans</i> as the main etiological pathogen, its impact at the tooth level is not fully understood. In this cross-sectional study, the levels and distribution of <i>S. mutans</i> in the posterior teeth at different dentition stages were investigated along with the corresponding tooth-specific microbiome. Occlusal plaque samples of 87 individual posterior teeth were collected from thirty children in three dentition stages (primary, mixed, and permanent). The <i>S. mutans</i> levels in the occlusal plaque of individual posterior teeth were quantified with qPCR, and those with preferential colonization were selected for tooth-specific microbiome analysis using 16S rRNA sequencing. Results: Quantification of <i>S. mutans</i> levels in the occlusal plaque confirmed the preferential colonization on the first primary and permanent molars. These teeth were selected for further tooth-specific microbiome sequencing, as they also displayed high caries experience. There were significant differences in the relative abundance of the four most abundant genera: <i>Neisseria</i>, <i>Streptococcus</i>, <i>Rothia,</i> and <i>Veillonella</i>. Furthermore, the tooth-level caries experience was correlated with a reduction in the microbiome diversity. Analyzing the different tooth-associated microbial communities, distinct tooth-specific core microbiomes were identified. Conclusions: Our findings suggest that caries susceptibility at the tooth level, depending on tooth type and dentition stage, is influenced by individual species as well as plaque community.https://www.mdpi.com/2076-2607/10/6/1129<i>Streptococcus mutans</i>dental cariesposterior teethdentition stagestooth-associated microbiome |
| spellingShingle | Márcia Dinis William Traynor Melissa Agnello Myung-Shin Sim Xuesong He Wenyuan Shi Renate Lux Nini Chaichanasakul Tran Tooth-Specific <i>Streptococcus mutans</i> Distribution and Associated Microbiome Microorganisms <i>Streptococcus mutans</i> dental caries posterior teeth dentition stages tooth-associated microbiome |
| title | Tooth-Specific <i>Streptococcus mutans</i> Distribution and Associated Microbiome |
| title_full | Tooth-Specific <i>Streptococcus mutans</i> Distribution and Associated Microbiome |
| title_fullStr | Tooth-Specific <i>Streptococcus mutans</i> Distribution and Associated Microbiome |
| title_full_unstemmed | Tooth-Specific <i>Streptococcus mutans</i> Distribution and Associated Microbiome |
| title_short | Tooth-Specific <i>Streptococcus mutans</i> Distribution and Associated Microbiome |
| title_sort | tooth specific i streptococcus mutans i distribution and associated microbiome |
| topic | <i>Streptococcus mutans</i> dental caries posterior teeth dentition stages tooth-associated microbiome |
| url | https://www.mdpi.com/2076-2607/10/6/1129 |
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