Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle <i>Platypus cylindrus</i> Fab. (Coleoptera: Curculionidae)
The ambrosia beetle <i>Platypus cylindrus</i> Fab. (Coleoptera: Curculionidae) is a major cork oak pest in Portugal. Female and male beetles have different roles in host tree colonization and are both equipped with prothoracic mycangia for fungal transportation. Despite a known beneficia...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/12/10/881 |
| _version_ | 1797514311692713984 |
|---|---|
| author | Stefano Nones Fernanda Simões Cândida Sofia Trindade José Matos Edmundo Sousa |
| author_facet | Stefano Nones Fernanda Simões Cândida Sofia Trindade José Matos Edmundo Sousa |
| author_sort | Stefano Nones |
| collection | DOAJ |
| description | The ambrosia beetle <i>Platypus cylindrus</i> Fab. (Coleoptera: Curculionidae) is a major cork oak pest in Portugal. Female and male beetles have different roles in host tree colonization and are both equipped with prothoracic mycangia for fungal transportation. Despite a known beneficial role of bacteria in ambrosia beetles, information on bacterial composition associated with prothoracic mycangia structures is scarce. Bacterial community from mycangia of <i>P. cylindrus</i> male and female beetles collected from cork oak galleries was investigated by means of 16S metagenomics. Mycangia anatomical structure was also explored with histological techniques and X-ray computed microtomography to highlight evidence supporting biological sexual dimorphism. A bacterial community with highly diverse bacterial taxa with low abundances at the genus level was revealed. Lactobacillales, <i>Leptotrichia</i>, <i>Neisseria</i>, <i>Rothia</i>, and Sphingomonadaceae were significantly more abundant in males, while <i>Acinetobacter</i>, Chitinophagaceae, Enterobacteriaceae, Erwiniaceae, Microbacteriaceae, and <i>Pseudoclavibacter</i> were more abundant in females. Additionally, a core bacteriome of five genera was shared by both sexes. Histological examination revealed visible connections linking external and internal tissues in females, but none in males. Overall, these results provide the first insights into sexual differentiation for bacteria in a Platypodinae beetle species, identifying key patterns of bacteria distribution in the context of beetle ecology and functional behavior. |
| first_indexed | 2024-03-10T06:30:13Z |
| format | Article |
| id | doaj.art-da83af4e4aa24cbda572c555dc4190aa |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T06:30:13Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-da83af4e4aa24cbda572c555dc4190aa2023-11-22T18:38:59ZengMDPI AGInsects2075-44502021-09-01121088110.3390/insects12100881Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle <i>Platypus cylindrus</i> Fab. (Coleoptera: Curculionidae)Stefano Nones0Fernanda Simões1Cândida Sofia Trindade2José Matos3Edmundo Sousa4Agrarian and Forestry Systems and Vegetal Health Unit, Instituto Nacional de Investigação Agrária e Veterinária (INIAV), Quinta do Marquês, 2780-159 Oeiras, PortugalBiotechnology and Genetic Resources Unit, Instituto Nacional de Investigação Agrária e Veterinária (INIAV), Quinta do Marquês, 2780-159 Oeiras, PortugalAgrarian and Forestry Systems and Vegetal Health Unit, Instituto Nacional de Investigação Agrária e Veterinária (INIAV), Quinta do Marquês, 2780-159 Oeiras, PortugalBiotechnology and Genetic Resources Unit, Instituto Nacional de Investigação Agrária e Veterinária (INIAV), Quinta do Marquês, 2780-159 Oeiras, PortugalAgrarian and Forestry Systems and Vegetal Health Unit, Instituto Nacional de Investigação Agrária e Veterinária (INIAV), Quinta do Marquês, 2780-159 Oeiras, PortugalThe ambrosia beetle <i>Platypus cylindrus</i> Fab. (Coleoptera: Curculionidae) is a major cork oak pest in Portugal. Female and male beetles have different roles in host tree colonization and are both equipped with prothoracic mycangia for fungal transportation. Despite a known beneficial role of bacteria in ambrosia beetles, information on bacterial composition associated with prothoracic mycangia structures is scarce. Bacterial community from mycangia of <i>P. cylindrus</i> male and female beetles collected from cork oak galleries was investigated by means of 16S metagenomics. Mycangia anatomical structure was also explored with histological techniques and X-ray computed microtomography to highlight evidence supporting biological sexual dimorphism. A bacterial community with highly diverse bacterial taxa with low abundances at the genus level was revealed. Lactobacillales, <i>Leptotrichia</i>, <i>Neisseria</i>, <i>Rothia</i>, and Sphingomonadaceae were significantly more abundant in males, while <i>Acinetobacter</i>, Chitinophagaceae, Enterobacteriaceae, Erwiniaceae, Microbacteriaceae, and <i>Pseudoclavibacter</i> were more abundant in females. Additionally, a core bacteriome of five genera was shared by both sexes. Histological examination revealed visible connections linking external and internal tissues in females, but none in males. Overall, these results provide the first insights into sexual differentiation for bacteria in a Platypodinae beetle species, identifying key patterns of bacteria distribution in the context of beetle ecology and functional behavior.https://www.mdpi.com/2075-4450/12/10/881sexual dimorphismmetabarcodingPlatypodinaeprokaryotescork oak |
| spellingShingle | Stefano Nones Fernanda Simões Cândida Sofia Trindade José Matos Edmundo Sousa Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle <i>Platypus cylindrus</i> Fab. (Coleoptera: Curculionidae) Insects sexual dimorphism metabarcoding Platypodinae prokaryotes cork oak |
| title | Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle <i>Platypus cylindrus</i> Fab. (Coleoptera: Curculionidae) |
| title_full | Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle <i>Platypus cylindrus</i> Fab. (Coleoptera: Curculionidae) |
| title_fullStr | Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle <i>Platypus cylindrus</i> Fab. (Coleoptera: Curculionidae) |
| title_full_unstemmed | Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle <i>Platypus cylindrus</i> Fab. (Coleoptera: Curculionidae) |
| title_short | Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle <i>Platypus cylindrus</i> Fab. (Coleoptera: Curculionidae) |
| title_sort | microbiome associated with the mycangia of female and male adults of the ambrosia beetle i platypus cylindrus i fab coleoptera curculionidae |
| topic | sexual dimorphism metabarcoding Platypodinae prokaryotes cork oak |
| url | https://www.mdpi.com/2075-4450/12/10/881 |
| work_keys_str_mv | AT stefanonones microbiomeassociatedwiththemycangiaoffemaleandmaleadultsoftheambrosiabeetleiplatypuscylindrusifabcoleopteracurculionidae AT fernandasimoes microbiomeassociatedwiththemycangiaoffemaleandmaleadultsoftheambrosiabeetleiplatypuscylindrusifabcoleopteracurculionidae AT candidasofiatrindade microbiomeassociatedwiththemycangiaoffemaleandmaleadultsoftheambrosiabeetleiplatypuscylindrusifabcoleopteracurculionidae AT josematos microbiomeassociatedwiththemycangiaoffemaleandmaleadultsoftheambrosiabeetleiplatypuscylindrusifabcoleopteracurculionidae AT edmundosousa microbiomeassociatedwiththemycangiaoffemaleandmaleadultsoftheambrosiabeetleiplatypuscylindrusifabcoleopteracurculionidae |