Gut microbial similarity in twins is driven by shared environment and aging
Summary: Background: Human gut microbiome composition is influenced by genetics, diet and environmental factors. We investigated the microbial composition in several gastrointestinal (GI) compartments to evaluate the impact of genetics, delivery mode, diet, household sharing and aging on microbial...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | EBioMedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396422001955 |
| _version_ | 1828490537805545472 |
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| author | Ramiro Vilchez-Vargas Jurgita Skieceviciene Konrad Lehr Greta Varkalaite Cosima Thon Mindaugas Urba Egidijus Morkūnas Laimutis Kucinskas Karolina Bauraite Denny Schanze Martin Zenker Peter Malfertheiner Juozas Kupcinskas Alexander Link |
| author_facet | Ramiro Vilchez-Vargas Jurgita Skieceviciene Konrad Lehr Greta Varkalaite Cosima Thon Mindaugas Urba Egidijus Morkūnas Laimutis Kucinskas Karolina Bauraite Denny Schanze Martin Zenker Peter Malfertheiner Juozas Kupcinskas Alexander Link |
| author_sort | Ramiro Vilchez-Vargas |
| collection | DOAJ |
| description | Summary: Background: Human gut microbiome composition is influenced by genetics, diet and environmental factors. We investigated the microbial composition in several gastrointestinal (GI) compartments to evaluate the impact of genetics, delivery mode, diet, household sharing and aging on microbial similarity in monozygotic and dizygotic twins. Methods: Fecal, biopsy and saliva samples were obtained from total 108 twins. DNA and/or RNA was extracted and the region V1-V2 of the 16S rRNA gene was amplified and sequenced. Bray-Curtis similarity was used for further microbiome comparisons, Mann-Whitney test was applied to evaluate the significant differences between groups and Spearman test was applied to reveal potential correlations between data. Findings: The global bacterial profiles were grouped into two clusters separating the upper and lower GI. The upper GI microbiome composition was strictly dependent on the Helicobacter pylori status. With a positivity rate of 55%, H. pylori completely colonized the stomach and separated infected twins from non-infected twins irrespective of zygosity status. Lower GI microbiome similarity between the twins was defined mainly by household-sharing and aging; whereas delivery mode and host genetics had no influence. There was a progredient decrease in the bacterial similarity with aging. Shared vs. non-shared phylotypes analysis showed that in both siblings the shared phylotypes progressively diminished with aging, while the non-shared phylotypes increased. Interpretation: Our findings strongly highlight the aging and shared household as they key determinants in gut microbial similarity and drift in twins irrespective of their zygotic state. Funding: This work was supported by the grant of the Research Council of Lithuania (Project no. APP-2/2016) and also partially supported by the funds of European Commission through the “European funds for regional development” (EFRE) as well as by the regional Ministry of Economy, Science and Digitalization as part of the “LiLife” Project as part of the “Autonomy in old Age” research group (Project ID: ZS/2018/11/95324). |
| first_indexed | 2024-12-11T10:45:22Z |
| format | Article |
| id | doaj.art-da849e27cbdb45b388f493aaa6d81e1c |
| institution | Directory Open Access Journal |
| issn | 2352-3964 |
| language | English |
| last_indexed | 2024-12-11T10:45:22Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EBioMedicine |
| spelling | doaj.art-da849e27cbdb45b388f493aaa6d81e1c2022-12-22T01:10:29ZengElsevierEBioMedicine2352-39642022-05-0179104011Gut microbial similarity in twins is driven by shared environment and agingRamiro Vilchez-Vargas0Jurgita Skieceviciene1Konrad Lehr2Greta Varkalaite3Cosima Thon4Mindaugas Urba5Egidijus Morkūnas6Laimutis Kucinskas7Karolina Bauraite8Denny Schanze9Martin Zenker10Peter Malfertheiner11Juozas Kupcinskas12Alexander Link13Department of Gastroenterology, Hepatology and Infectious Diseases, Section of Molecular Gastroenterology and Microbiota-associated Diseases, Otto-von-Guericke University, Leipziger Str. 44, Magdeburg 39120, GermanyDepartment of Gastroenterology, Lithuanian University of Health Sciences, Kaunas 44307, Lithuania; Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas 44307, LithuaniaDepartment of Gastroenterology, Hepatology and Infectious Diseases, Section of Molecular Gastroenterology and Microbiota-associated Diseases, Otto-von-Guericke University, Leipziger Str. 44, Magdeburg 39120, GermanyInstitute for Digestive Research, Lithuanian University of Health Sciences, Kaunas 44307, LithuaniaDepartment of Gastroenterology, Hepatology and Infectious Diseases, Section of Molecular Gastroenterology and Microbiota-associated Diseases, Otto-von-Guericke University, Leipziger Str. 44, Magdeburg 39120, GermanyDepartment of Gastroenterology, Lithuanian University of Health Sciences, Kaunas 44307, Lithuania; Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas 44307, LithuaniaDepartment of Gastroenterology, Lithuanian University of Health Sciences, Kaunas 44307, Lithuania; Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas 44307, LithuaniaInstitute of Biology Systems and Genetic Research, Lithuanian University of Health Sciences, 44307 Kaunas, LithuaniaDepartment of Gastroenterology, Lithuanian University of Health Sciences, Kaunas 44307, LithuaniaInstitute of Human Genetics, Otto-von-Guericke University Magdeburg, GermanyInstitute of Human Genetics, Otto-von-Guericke University Magdeburg, GermanyDepartment of Gastroenterology, Hepatology and Infectious Diseases, Section of Molecular Gastroenterology and Microbiota-associated Diseases, Otto-von-Guericke University, Leipziger Str. 44, Magdeburg 39120, GermanyDepartment of Gastroenterology, Lithuanian University of Health Sciences, Kaunas 44307, Lithuania; Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas 44307, Lithuania; Corresponding author at: Department of Gastroenterology, Lithuanian University of Health Sciences, Kaunas 44307, Lithuania.Department of Gastroenterology, Hepatology and Infectious Diseases, Section of Molecular Gastroenterology and Microbiota-associated Diseases, Otto-von-Guericke University, Leipziger Str. 44, Magdeburg 39120, Germany; Corresponding author at: Department of Gastroenterology, Hepatology and Infectious Diseases, Section of Molecular Gastroenterology and Microbiota-associated Diseases, Otto-von-Guericke University, Leipziger Str. 44, Magdeburg 39120, GermanySummary: Background: Human gut microbiome composition is influenced by genetics, diet and environmental factors. We investigated the microbial composition in several gastrointestinal (GI) compartments to evaluate the impact of genetics, delivery mode, diet, household sharing and aging on microbial similarity in monozygotic and dizygotic twins. Methods: Fecal, biopsy and saliva samples were obtained from total 108 twins. DNA and/or RNA was extracted and the region V1-V2 of the 16S rRNA gene was amplified and sequenced. Bray-Curtis similarity was used for further microbiome comparisons, Mann-Whitney test was applied to evaluate the significant differences between groups and Spearman test was applied to reveal potential correlations between data. Findings: The global bacterial profiles were grouped into two clusters separating the upper and lower GI. The upper GI microbiome composition was strictly dependent on the Helicobacter pylori status. With a positivity rate of 55%, H. pylori completely colonized the stomach and separated infected twins from non-infected twins irrespective of zygosity status. Lower GI microbiome similarity between the twins was defined mainly by household-sharing and aging; whereas delivery mode and host genetics had no influence. There was a progredient decrease in the bacterial similarity with aging. Shared vs. non-shared phylotypes analysis showed that in both siblings the shared phylotypes progressively diminished with aging, while the non-shared phylotypes increased. Interpretation: Our findings strongly highlight the aging and shared household as they key determinants in gut microbial similarity and drift in twins irrespective of their zygotic state. Funding: This work was supported by the grant of the Research Council of Lithuania (Project no. APP-2/2016) and also partially supported by the funds of European Commission through the “European funds for regional development” (EFRE) as well as by the regional Ministry of Economy, Science and Digitalization as part of the “LiLife” Project as part of the “Autonomy in old Age” research group (Project ID: ZS/2018/11/95324).http://www.sciencedirect.com/science/article/pii/S2352396422001955Microbiome16S rRNA sequencingAgingShared householdStomachHelicobacter pylori |
| spellingShingle | Ramiro Vilchez-Vargas Jurgita Skieceviciene Konrad Lehr Greta Varkalaite Cosima Thon Mindaugas Urba Egidijus Morkūnas Laimutis Kucinskas Karolina Bauraite Denny Schanze Martin Zenker Peter Malfertheiner Juozas Kupcinskas Alexander Link Gut microbial similarity in twins is driven by shared environment and aging EBioMedicine Microbiome 16S rRNA sequencing Aging Shared household Stomach Helicobacter pylori |
| title | Gut microbial similarity in twins is driven by shared environment and aging |
| title_full | Gut microbial similarity in twins is driven by shared environment and aging |
| title_fullStr | Gut microbial similarity in twins is driven by shared environment and aging |
| title_full_unstemmed | Gut microbial similarity in twins is driven by shared environment and aging |
| title_short | Gut microbial similarity in twins is driven by shared environment and aging |
| title_sort | gut microbial similarity in twins is driven by shared environment and aging |
| topic | Microbiome 16S rRNA sequencing Aging Shared household Stomach Helicobacter pylori |
| url | http://www.sciencedirect.com/science/article/pii/S2352396422001955 |
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