Detection of Paenibacillus larvae spores in honey by conventional PCR and its potential for American foulbrood control
The present study attempted to detect Paenibacillus larvae spores in naturally contaminated honeys by conventional PCR and to determine the sensitivity of the reaction with different primer pairs in order to assess its potential for American foulbrood control. For this purpose, duplicated honey sam-...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria
2019-06-01
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| Series: | Bulgarian Journal of Veterinary Medicine |
| Subjects: | |
| Online Access: | http://tru.uni-sz.bg/bjvm/BJVM%20June%202019%20p.163-170.pdf |
| _version_ | 1819028116925841408 |
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| author | N. Rusenova P. Parvanov S. Stanilova |
| author_facet | N. Rusenova P. Parvanov S. Stanilova |
| author_sort | N. Rusenova |
| collection | DOAJ |
| description | The present study attempted to detect Paenibacillus larvae spores in naturally contaminated honeys by conventional PCR and to determine the sensitivity of the reaction with different primer pairs in order to assess its potential for American foulbrood control. For this purpose, duplicated honey sam-ples were collected from 5 bee colonies with clinical American foulbrood and 5 clinically healthy colonies in the same apiary. The samples were analysed for the presence of Paenibacillus larvae spores by culture method and subsequent PCR detection in bacterial colonies. The PCR performed directly with spore DNA failed in 6 out of the 20 honeys investigated with spore load of 10–46 cfu/g. The established sensitivity of 70% of the reaction in the present study shows that the adequate control of American foulbrood by analysis of honeys for Paenibacillus larvae spore contamination should be done by combination of culture method followed by PCR in bacterial colonies, whose sensitivity was 100%. |
| first_indexed | 2024-12-21T05:53:15Z |
| format | Article |
| id | doaj.art-da89ab77469d4896890093be9c2a27cc |
| institution | Directory Open Access Journal |
| issn | 1311-1477 1313-3543 |
| language | English |
| last_indexed | 2024-12-21T05:53:15Z |
| publishDate | 2019-06-01 |
| publisher | Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria |
| record_format | Article |
| series | Bulgarian Journal of Veterinary Medicine |
| spelling | doaj.art-da89ab77469d4896890093be9c2a27cc2022-12-21T19:13:55ZengFaculty of Veterinary Medicine, Trakia University, Stara Zagora, BulgariaBulgarian Journal of Veterinary Medicine1311-14771313-35432019-06-0122216317010.15547/bjvm.2159Detection of Paenibacillus larvae spores in honey by conventional PCR and its potential for American foulbrood controlN. Rusenova0P. Parvanov1S. Stanilova2Faculty of Veterinary Medicine, Trakia University, Stara Zagora, BulgariaFaculty of Veterinary Medicine, Trakia University, Stara Zagora, BulgariaFaculty of Medicine, Trakia University, Stara Zagora, BulgariaThe present study attempted to detect Paenibacillus larvae spores in naturally contaminated honeys by conventional PCR and to determine the sensitivity of the reaction with different primer pairs in order to assess its potential for American foulbrood control. For this purpose, duplicated honey sam-ples were collected from 5 bee colonies with clinical American foulbrood and 5 clinically healthy colonies in the same apiary. The samples were analysed for the presence of Paenibacillus larvae spores by culture method and subsequent PCR detection in bacterial colonies. The PCR performed directly with spore DNA failed in 6 out of the 20 honeys investigated with spore load of 10–46 cfu/g. The established sensitivity of 70% of the reaction in the present study shows that the adequate control of American foulbrood by analysis of honeys for Paenibacillus larvae spore contamination should be done by combination of culture method followed by PCR in bacterial colonies, whose sensitivity was 100%.http://tru.uni-sz.bg/bjvm/BJVM%20June%202019%20p.163-170.pdfAmerican foulbroodcontrolhoneyPaenibacillus larvaePCRspores |
| spellingShingle | N. Rusenova P. Parvanov S. Stanilova Detection of Paenibacillus larvae spores in honey by conventional PCR and its potential for American foulbrood control Bulgarian Journal of Veterinary Medicine American foulbrood control honey Paenibacillus larvae PCR spores |
| title | Detection of Paenibacillus larvae spores in honey by conventional PCR and its potential for American foulbrood control |
| title_full | Detection of Paenibacillus larvae spores in honey by conventional PCR and its potential for American foulbrood control |
| title_fullStr | Detection of Paenibacillus larvae spores in honey by conventional PCR and its potential for American foulbrood control |
| title_full_unstemmed | Detection of Paenibacillus larvae spores in honey by conventional PCR and its potential for American foulbrood control |
| title_short | Detection of Paenibacillus larvae spores in honey by conventional PCR and its potential for American foulbrood control |
| title_sort | detection of paenibacillus larvae spores in honey by conventional pcr and its potential for american foulbrood control |
| topic | American foulbrood control honey Paenibacillus larvae PCR spores |
| url | http://tru.uni-sz.bg/bjvm/BJVM%20June%202019%20p.163-170.pdf |
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