Distribution, Polymorphism and Function Characteristics of the GST-Encoding <i>Fhb7</i> in <i>Triticeae</i>
Encoding a glutathione S-transferase (GST) and conferring resistance to Fusarium head blight (FHB), <i>Fhb7</i> was successfully isolated from the newly assembled <i>Thinopyrum elongatum</i> genome by researchers, with blasting searches revealing that <i>Thinopyrum</...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/16/2074 |
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| author | Xianrui Guo Mian Wang Houyang Kang Yonghong Zhou Fangpu Han |
| author_facet | Xianrui Guo Mian Wang Houyang Kang Yonghong Zhou Fangpu Han |
| author_sort | Xianrui Guo |
| collection | DOAJ |
| description | Encoding a glutathione S-transferase (GST) and conferring resistance to Fusarium head blight (FHB), <i>Fhb7</i> was successfully isolated from the newly assembled <i>Thinopyrum elongatum</i> genome by researchers, with blasting searches revealing that <i>Thinopyrum</i> gained <i>Fhb7</i> through horizontal gene transfer from an endophytic <i>Epichloë</i> species. On the contrary, our molecular evidence reveals that the homologs of <i>Fhb7</i> are distributed commonly in <i>Triticeae</i>. Other than <i>Thinopyrum</i>, the <i>Fhb7</i> homologs were also detected in four other genera, <i>Elymus</i>, <i>Leymus</i>, <i>Roegneria</i> and <i>Pseudoroegneria</i>, respectively. Sequence comparisons revealed that the protein sequences were at least 94% identical across all of the <i>Fhb7</i> homologs in <i>Triticeae</i> plants, which in turn suggested that the horizontal gene transfer of the <i>Fhb7</i> might have occurred before <i>Triticeae</i> differentiation instead of <i>Thinopyrum</i>. The multiple <i>Fhb7</i> homologs detected in some <i>Triticeae</i> accessions and wheat-<i>Thinopyrum</i> derivatives might be attributed to the alloploid nature and gene duplication during evolution. In addition, we discovered that some wheat-<i>Thinopyrum</i> derivatives carrying the <i>Fhb7</i> homologs had a completely different reaction to Fusarium head blight, which made us question the ability of the GST-encoding <i>Fhb7</i> to resist FHB. |
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| language | English |
| last_indexed | 2024-03-09T09:51:21Z |
| publishDate | 2022-08-01 |
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| series | Plants |
| spelling | doaj.art-da8a1790b0814b06baaa2810244fd14c2023-12-02T00:10:47ZengMDPI AGPlants2223-77472022-08-011116207410.3390/plants11162074Distribution, Polymorphism and Function Characteristics of the GST-Encoding <i>Fhb7</i> in <i>Triticeae</i>Xianrui Guo0Mian Wang1Houyang Kang2Yonghong Zhou3Fangpu Han4State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaEncoding a glutathione S-transferase (GST) and conferring resistance to Fusarium head blight (FHB), <i>Fhb7</i> was successfully isolated from the newly assembled <i>Thinopyrum elongatum</i> genome by researchers, with blasting searches revealing that <i>Thinopyrum</i> gained <i>Fhb7</i> through horizontal gene transfer from an endophytic <i>Epichloë</i> species. On the contrary, our molecular evidence reveals that the homologs of <i>Fhb7</i> are distributed commonly in <i>Triticeae</i>. Other than <i>Thinopyrum</i>, the <i>Fhb7</i> homologs were also detected in four other genera, <i>Elymus</i>, <i>Leymus</i>, <i>Roegneria</i> and <i>Pseudoroegneria</i>, respectively. Sequence comparisons revealed that the protein sequences were at least 94% identical across all of the <i>Fhb7</i> homologs in <i>Triticeae</i> plants, which in turn suggested that the horizontal gene transfer of the <i>Fhb7</i> might have occurred before <i>Triticeae</i> differentiation instead of <i>Thinopyrum</i>. The multiple <i>Fhb7</i> homologs detected in some <i>Triticeae</i> accessions and wheat-<i>Thinopyrum</i> derivatives might be attributed to the alloploid nature and gene duplication during evolution. In addition, we discovered that some wheat-<i>Thinopyrum</i> derivatives carrying the <i>Fhb7</i> homologs had a completely different reaction to Fusarium head blight, which made us question the ability of the GST-encoding <i>Fhb7</i> to resist FHB.https://www.mdpi.com/2223-7747/11/16/2074horizontal gene transfer<i>Fhb7</i><i>Triticeae</i><i>Thinopyrum</i>Fusarium head blight |
| spellingShingle | Xianrui Guo Mian Wang Houyang Kang Yonghong Zhou Fangpu Han Distribution, Polymorphism and Function Characteristics of the GST-Encoding <i>Fhb7</i> in <i>Triticeae</i> Plants horizontal gene transfer <i>Fhb7</i> <i>Triticeae</i> <i>Thinopyrum</i> Fusarium head blight |
| title | Distribution, Polymorphism and Function Characteristics of the GST-Encoding <i>Fhb7</i> in <i>Triticeae</i> |
| title_full | Distribution, Polymorphism and Function Characteristics of the GST-Encoding <i>Fhb7</i> in <i>Triticeae</i> |
| title_fullStr | Distribution, Polymorphism and Function Characteristics of the GST-Encoding <i>Fhb7</i> in <i>Triticeae</i> |
| title_full_unstemmed | Distribution, Polymorphism and Function Characteristics of the GST-Encoding <i>Fhb7</i> in <i>Triticeae</i> |
| title_short | Distribution, Polymorphism and Function Characteristics of the GST-Encoding <i>Fhb7</i> in <i>Triticeae</i> |
| title_sort | distribution polymorphism and function characteristics of the gst encoding i fhb7 i in i triticeae i |
| topic | horizontal gene transfer <i>Fhb7</i> <i>Triticeae</i> <i>Thinopyrum</i> Fusarium head blight |
| url | https://www.mdpi.com/2223-7747/11/16/2074 |
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