Bactericidal activities of the cationic steroid CSA-13 and the cathelicidin peptide LL-37 against <it>Helicobacter pylori </it>in simulated gastric juice
<p>Abstract</p> <p>Background</p> <p>The worldwide appearance of drug-resistant strains of <it>H. pylori </it>motivates a search for new agents with therapeutic potential against this family of bacteria that colonizes the stomach, and is associated with aden...
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| Format: | Article |
| Language: | English |
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BMC
2009-09-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/9/187 |
| _version_ | 1818472699087814656 |
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| author | Janmey Paul A Diamond Scott Savage Paul B Namiot Zbigniew Wen Qi Fein David E Namiot Andrzej Leszczyńska Katarzyna Bucki Robert |
| author_facet | Janmey Paul A Diamond Scott Savage Paul B Namiot Zbigniew Wen Qi Fein David E Namiot Andrzej Leszczyńska Katarzyna Bucki Robert |
| author_sort | Janmey Paul A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The worldwide appearance of drug-resistant strains of <it>H. pylori </it>motivates a search for new agents with therapeutic potential against this family of bacteria that colonizes the stomach, and is associated with adenocarcinoma development. This study was designed to assess <it>in vitro </it>the anti-<it>H. pylori </it>potential of cathelicidin LL-37 peptide, which is naturally present in gastric juice, its optimized synthetic analog WLBU2, and the non-peptide antibacterial agent ceragenin CSA-13.</p> <p>Results</p> <p>In agreement with previous studies, increased expression of hCAP-18/LL-37 was observed in gastric mucosa obtained from <it>H. pylori </it>infected subjects. MBC (minimum bactericidal concentration) values determined in nutrient-containing media range from 100-800 μg/ml for LL-37, 17.8-142 μg/ml for WLBU2 and 0.275-8.9 μg/ml for ceragenin CSA-13. These data indicate substantial, but widely differing antibacterial activities against clinical isolates of <it>H. pylori</it>. After incubation in simulated gastric juice (low pH with presence of pepsin) CSA-13, but not LL-37 or WLBU2, retained antibacterial activity. Compared to LL-37 and WLBU2 peptides, CSA-13 activity was also more resistant to inhibition by isolated host gastric mucins.</p> <p>Conclusion</p> <p>These data indicate that cholic acid-based antimicrobial agents such as CSA-13 resist proteolytic degradation and inhibition by mucin and have potential for treatment of <it>H. pylori </it>infections, including those caused by the clarithromycin and/or metronidazole-resistant strains.</p> |
| first_indexed | 2024-04-14T04:11:10Z |
| format | Article |
| id | doaj.art-da8b13bbea884518aa305beeb2a23341 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-14T04:11:10Z |
| publishDate | 2009-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-da8b13bbea884518aa305beeb2a233412022-12-22T02:13:10ZengBMCBMC Microbiology1471-21802009-09-019118710.1186/1471-2180-9-187Bactericidal activities of the cationic steroid CSA-13 and the cathelicidin peptide LL-37 against <it>Helicobacter pylori </it>in simulated gastric juiceJanmey Paul ADiamond ScottSavage Paul BNamiot ZbigniewWen QiFein David ENamiot AndrzejLeszczyńska KatarzynaBucki Robert<p>Abstract</p> <p>Background</p> <p>The worldwide appearance of drug-resistant strains of <it>H. pylori </it>motivates a search for new agents with therapeutic potential against this family of bacteria that colonizes the stomach, and is associated with adenocarcinoma development. This study was designed to assess <it>in vitro </it>the anti-<it>H. pylori </it>potential of cathelicidin LL-37 peptide, which is naturally present in gastric juice, its optimized synthetic analog WLBU2, and the non-peptide antibacterial agent ceragenin CSA-13.</p> <p>Results</p> <p>In agreement with previous studies, increased expression of hCAP-18/LL-37 was observed in gastric mucosa obtained from <it>H. pylori </it>infected subjects. MBC (minimum bactericidal concentration) values determined in nutrient-containing media range from 100-800 μg/ml for LL-37, 17.8-142 μg/ml for WLBU2 and 0.275-8.9 μg/ml for ceragenin CSA-13. These data indicate substantial, but widely differing antibacterial activities against clinical isolates of <it>H. pylori</it>. After incubation in simulated gastric juice (low pH with presence of pepsin) CSA-13, but not LL-37 or WLBU2, retained antibacterial activity. Compared to LL-37 and WLBU2 peptides, CSA-13 activity was also more resistant to inhibition by isolated host gastric mucins.</p> <p>Conclusion</p> <p>These data indicate that cholic acid-based antimicrobial agents such as CSA-13 resist proteolytic degradation and inhibition by mucin and have potential for treatment of <it>H. pylori </it>infections, including those caused by the clarithromycin and/or metronidazole-resistant strains.</p>http://www.biomedcentral.com/1471-2180/9/187 |
| spellingShingle | Janmey Paul A Diamond Scott Savage Paul B Namiot Zbigniew Wen Qi Fein David E Namiot Andrzej Leszczyńska Katarzyna Bucki Robert Bactericidal activities of the cationic steroid CSA-13 and the cathelicidin peptide LL-37 against <it>Helicobacter pylori </it>in simulated gastric juice BMC Microbiology |
| title | Bactericidal activities of the cationic steroid CSA-13 and the cathelicidin peptide LL-37 against <it>Helicobacter pylori </it>in simulated gastric juice |
| title_full | Bactericidal activities of the cationic steroid CSA-13 and the cathelicidin peptide LL-37 against <it>Helicobacter pylori </it>in simulated gastric juice |
| title_fullStr | Bactericidal activities of the cationic steroid CSA-13 and the cathelicidin peptide LL-37 against <it>Helicobacter pylori </it>in simulated gastric juice |
| title_full_unstemmed | Bactericidal activities of the cationic steroid CSA-13 and the cathelicidin peptide LL-37 against <it>Helicobacter pylori </it>in simulated gastric juice |
| title_short | Bactericidal activities of the cationic steroid CSA-13 and the cathelicidin peptide LL-37 against <it>Helicobacter pylori </it>in simulated gastric juice |
| title_sort | bactericidal activities of the cationic steroid csa 13 and the cathelicidin peptide ll 37 against it helicobacter pylori it in simulated gastric juice |
| url | http://www.biomedcentral.com/1471-2180/9/187 |
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