Cerebrospinal Fluid in Classical Trigeminal Neuralgia: An Exploratory Study on Candidate Biomarkers
Trigeminal neuralgia (TN) is a severe type of facial pain. A neurovascular conflict between cranial nerve V and a nearby vessel is the main pathophysiological mechanism, but additional factors are likely necessary to elicit TN. In this study, the primary aim was to explore differences in protein exp...
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MDPI AG
2022-04-01
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| author | Teodor Svedung Wettervik Dick Folkvaljon Torsten Gordh Eva Freyhult Kim Kultima Hans Ericson Sami Abu Hamdeh |
| author_facet | Teodor Svedung Wettervik Dick Folkvaljon Torsten Gordh Eva Freyhult Kim Kultima Hans Ericson Sami Abu Hamdeh |
| author_sort | Teodor Svedung Wettervik |
| collection | DOAJ |
| description | Trigeminal neuralgia (TN) is a severe type of facial pain. A neurovascular conflict between cranial nerve V and a nearby vessel is the main pathophysiological mechanism, but additional factors are likely necessary to elicit TN. In this study, the primary aim was to explore differences in protein expression in the cerebrospinal fluid (CSF) of TN patients in relation to controls. <b>Methods:</b> Sixteen TN patients treated with microvascular decompression and 16 control patients undergoing spinal anesthesia for urological conditions were included. Lumbar CSF was collected preoperatively for the TN patients and before spinal anesthesia for the controls. A multiplexed proximity extension analysis of 91 CSF proteins was conducted using Proseek Multiplex Development 96, including biomarkers of cell communication, cell death, neurogenesis, and inflammation <b>Results:</b> The TN patients and the controls were of similar age, sex, and burden of co-morbidities. The TN patients exhibited higher concentrations of Clec11a, LGMN, MFG-E8, and ANGPTL-4 in CSF than the controls (q < 0.05). <b>Conclusions:</b> TN patients exhibited increased CSF biomarkers indicative of peripheral demyelinating injury (Clec11a), immune tolerance and destruction of myelin (LGMN), neuronal cell death (MFG-E8), and disturbances in myelin clearance (ANGPTL-8). Our findings are hypothesis-generating for candidate biomarkers and pathophysiological processes in classical TN. |
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| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T03:17:54Z |
| publishDate | 2022-04-01 |
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| series | Biomedicines |
| spelling | doaj.art-da8b4d46eca24afca2247b2c7c4ce76d2023-11-23T10:09:35ZengMDPI AGBiomedicines2227-90592022-04-0110599810.3390/biomedicines10050998Cerebrospinal Fluid in Classical Trigeminal Neuralgia: An Exploratory Study on Candidate BiomarkersTeodor Svedung Wettervik0Dick Folkvaljon1Torsten Gordh2Eva Freyhult3Kim Kultima4Hans Ericson5Sami Abu Hamdeh6Department of Medical Sciences, Section of Neurosurgery, Uppsala University, SE-751 85 Uppsala, SwedenDepartment of Medical Sciences, Section of Neurosurgery, Uppsala University, SE-751 85 Uppsala, SwedenDepartment of Surgical Sciences, Pain Research, Anesthesiology and Intensive Care, Uppsala University, SE-751 85 Uppsala, SwedenDepartment of Cell and Molecular Biology, Uppsala University, 75124 Uppsala, SwedenDepartment of Medical Sciences, Chemical Chemistry, Uppsala University, SE-751 85 Uppsala, SwedenDepartment of Medical Sciences, Section of Neurosurgery, Uppsala University, SE-751 85 Uppsala, SwedenDepartment of Medical Sciences, Section of Neurosurgery, Uppsala University, SE-751 85 Uppsala, SwedenTrigeminal neuralgia (TN) is a severe type of facial pain. A neurovascular conflict between cranial nerve V and a nearby vessel is the main pathophysiological mechanism, but additional factors are likely necessary to elicit TN. In this study, the primary aim was to explore differences in protein expression in the cerebrospinal fluid (CSF) of TN patients in relation to controls. <b>Methods:</b> Sixteen TN patients treated with microvascular decompression and 16 control patients undergoing spinal anesthesia for urological conditions were included. Lumbar CSF was collected preoperatively for the TN patients and before spinal anesthesia for the controls. A multiplexed proximity extension analysis of 91 CSF proteins was conducted using Proseek Multiplex Development 96, including biomarkers of cell communication, cell death, neurogenesis, and inflammation <b>Results:</b> The TN patients and the controls were of similar age, sex, and burden of co-morbidities. The TN patients exhibited higher concentrations of Clec11a, LGMN, MFG-E8, and ANGPTL-4 in CSF than the controls (q < 0.05). <b>Conclusions:</b> TN patients exhibited increased CSF biomarkers indicative of peripheral demyelinating injury (Clec11a), immune tolerance and destruction of myelin (LGMN), neuronal cell death (MFG-E8), and disturbances in myelin clearance (ANGPTL-8). Our findings are hypothesis-generating for candidate biomarkers and pathophysiological processes in classical TN.https://www.mdpi.com/2227-9059/10/5/998cerebrospinal fluiddemyelinationneuroinflammationtrigeminal neuralgia |
| spellingShingle | Teodor Svedung Wettervik Dick Folkvaljon Torsten Gordh Eva Freyhult Kim Kultima Hans Ericson Sami Abu Hamdeh Cerebrospinal Fluid in Classical Trigeminal Neuralgia: An Exploratory Study on Candidate Biomarkers Biomedicines cerebrospinal fluid demyelination neuroinflammation trigeminal neuralgia |
| title | Cerebrospinal Fluid in Classical Trigeminal Neuralgia: An Exploratory Study on Candidate Biomarkers |
| title_full | Cerebrospinal Fluid in Classical Trigeminal Neuralgia: An Exploratory Study on Candidate Biomarkers |
| title_fullStr | Cerebrospinal Fluid in Classical Trigeminal Neuralgia: An Exploratory Study on Candidate Biomarkers |
| title_full_unstemmed | Cerebrospinal Fluid in Classical Trigeminal Neuralgia: An Exploratory Study on Candidate Biomarkers |
| title_short | Cerebrospinal Fluid in Classical Trigeminal Neuralgia: An Exploratory Study on Candidate Biomarkers |
| title_sort | cerebrospinal fluid in classical trigeminal neuralgia an exploratory study on candidate biomarkers |
| topic | cerebrospinal fluid demyelination neuroinflammation trigeminal neuralgia |
| url | https://www.mdpi.com/2227-9059/10/5/998 |
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