The Marine Seagrass <i>Halophila stipulacea</i> as a Source of Bioactive Metabolites against Obesity and Biofouling

Marine organisms, including seagrasses, are important sources of biologically active molecules for the treatment of human diseases. In this study, organic extracts of the marine seagrass <i>Halophila stipulacea</i> obtained by different polarities from leaves (L) and stems (S) (hexane [H...

Full description

Bibliographic Details
Main Authors: Sawssen Bel Mabrouk, Mariana Reis, Maria Lígia Sousa, Tiago Ribeiro, Joana R. Almeida, Sandra Pereira, Jorge Antunes, Filipa Rosa, Vitor Vasconcelos, Lotfi Achour, Adnen Kacem, Ralph Urbatzka
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:Marine Drugs
Subjects:
Online Access:https://www.mdpi.com/1660-3397/18/2/88
_version_ 1818001051656126464
author Sawssen Bel Mabrouk
Mariana Reis
Maria Lígia Sousa
Tiago Ribeiro
Joana R. Almeida
Sandra Pereira
Jorge Antunes
Filipa Rosa
Vitor Vasconcelos
Lotfi Achour
Adnen Kacem
Ralph Urbatzka
author_facet Sawssen Bel Mabrouk
Mariana Reis
Maria Lígia Sousa
Tiago Ribeiro
Joana R. Almeida
Sandra Pereira
Jorge Antunes
Filipa Rosa
Vitor Vasconcelos
Lotfi Achour
Adnen Kacem
Ralph Urbatzka
author_sort Sawssen Bel Mabrouk
collection DOAJ
description Marine organisms, including seagrasses, are important sources of biologically active molecules for the treatment of human diseases. In this study, organic extracts of the marine seagrass <i>Halophila stipulacea</i> obtained by different polarities from leaves (L) and stems (S) (hexane [HL, HS], ethyl acetate [EL, ES], and methanol [ML, MS]) were tested for different bioactivities. The screening comprehended the cytotoxicity activity against cancer cell lines grown as a monolayer culture or as multicellular spheroids (cancer), glucose uptake in cells (diabetes), reduction of lipid content in fatty acid-overloaded liver cells (steatosis), and lipid-reducing activity in zebrafish larvae (obesity), as well as the antifouling activity against marine bacteria (microfouling) and mussel larval settlement (macrofouling). HL, EL, HS, and ES extracts showed statistically significant cytotoxicity against cancer cell lines. The extracts did not have any significant effect on glucose uptake and on the reduction of lipids in liver cells. The EL and ML extracts reduced neutral lipid contents on the larvae of zebrafish with EC<sub>50</sub> values of 2.2 &#181;g/mL for EL and 1.2 &#181;g/mL for ML. For the antifouling activity, the HS and ML extracts showed a significant inhibitory effect (<i>p</i> &lt; 0.05) against the settlement of <i>Mytilus galloprovincialis</i> plantigrade larvae. The metabolite profiling using HR-LC-MS/MS and GNPS (The Global Natural Product Social Molecular Networking) analyses identified a variety of known primary and secondary metabolites in the extracts, along with some unreported molecules. Various compounds were detected with known activities on cancer (polyphenols: Luteolin, apeginin, matairesinol), on metabolic diseases (polyphenols: cirsimarin, spiraeoside, 2,4-dihydroxyheptadec-16-ynyl acetate; amino acids: N-acetyl-L-tyrosine), or on antifouling (fatty acids: 13-decosenamide; cinnamic acids: 3-hydroxy-4-methoxycinnamic acid, alpha-cyano-4-hydroxycinnamic), which could be, in part, responsible for the observed bioactivities. In summary, this study revealed that <i>Halophila stipulacea</i> is a rich source of metabolites with promising activities against obesity and biofouling and suggests that this seagrass could be useful for drug discovery in the future.
first_indexed 2024-04-14T03:29:30Z
format Article
id doaj.art-da9017006d5746a5b3d961f6d68a6797
institution Directory Open Access Journal
issn 1660-3397
language English
last_indexed 2024-04-14T03:29:30Z
publishDate 2020-01-01
publisher MDPI AG
record_format Article
series Marine Drugs
spelling doaj.art-da9017006d5746a5b3d961f6d68a67972022-12-22T02:15:01ZengMDPI AGMarine Drugs1660-33972020-01-011828810.3390/md18020088md18020088The Marine Seagrass <i>Halophila stipulacea</i> as a Source of Bioactive Metabolites against Obesity and BiofoulingSawssen Bel Mabrouk0Mariana Reis1Maria Lígia Sousa2Tiago Ribeiro3Joana R. Almeida4Sandra Pereira5Jorge Antunes6Filipa Rosa7Vitor Vasconcelos8Lotfi Achour9Adnen Kacem10Ralph Urbatzka11Research Laboratory (LR14-ES06): Bioresources: Integrative Biology and Valorization, High Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, TunisiaInterdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Avenida General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Avenida General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Avenida General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Avenida General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Avenida General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Avenida General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Avenida General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Avenida General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalResearch Laboratory (LR14-ES06): Bioresources: Integrative Biology and Valorization, High Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, TunisiaResearch Laboratory (LR14-ES06): Bioresources: Integrative Biology and Valorization, High Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, TunisiaInterdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Avenida General Norton de Matos, s/n, 4450-208 Matosinhos, PortugalMarine organisms, including seagrasses, are important sources of biologically active molecules for the treatment of human diseases. In this study, organic extracts of the marine seagrass <i>Halophila stipulacea</i> obtained by different polarities from leaves (L) and stems (S) (hexane [HL, HS], ethyl acetate [EL, ES], and methanol [ML, MS]) were tested for different bioactivities. The screening comprehended the cytotoxicity activity against cancer cell lines grown as a monolayer culture or as multicellular spheroids (cancer), glucose uptake in cells (diabetes), reduction of lipid content in fatty acid-overloaded liver cells (steatosis), and lipid-reducing activity in zebrafish larvae (obesity), as well as the antifouling activity against marine bacteria (microfouling) and mussel larval settlement (macrofouling). HL, EL, HS, and ES extracts showed statistically significant cytotoxicity against cancer cell lines. The extracts did not have any significant effect on glucose uptake and on the reduction of lipids in liver cells. The EL and ML extracts reduced neutral lipid contents on the larvae of zebrafish with EC<sub>50</sub> values of 2.2 &#181;g/mL for EL and 1.2 &#181;g/mL for ML. For the antifouling activity, the HS and ML extracts showed a significant inhibitory effect (<i>p</i> &lt; 0.05) against the settlement of <i>Mytilus galloprovincialis</i> plantigrade larvae. The metabolite profiling using HR-LC-MS/MS and GNPS (The Global Natural Product Social Molecular Networking) analyses identified a variety of known primary and secondary metabolites in the extracts, along with some unreported molecules. Various compounds were detected with known activities on cancer (polyphenols: Luteolin, apeginin, matairesinol), on metabolic diseases (polyphenols: cirsimarin, spiraeoside, 2,4-dihydroxyheptadec-16-ynyl acetate; amino acids: N-acetyl-L-tyrosine), or on antifouling (fatty acids: 13-decosenamide; cinnamic acids: 3-hydroxy-4-methoxycinnamic acid, alpha-cyano-4-hydroxycinnamic), which could be, in part, responsible for the observed bioactivities. In summary, this study revealed that <i>Halophila stipulacea</i> is a rich source of metabolites with promising activities against obesity and biofouling and suggests that this seagrass could be useful for drug discovery in the future.https://www.mdpi.com/1660-3397/18/2/88<i>halophila stipulacea</i>extractsbioactivitycytotoxicityanti-obesityantifouling
spellingShingle Sawssen Bel Mabrouk
Mariana Reis
Maria Lígia Sousa
Tiago Ribeiro
Joana R. Almeida
Sandra Pereira
Jorge Antunes
Filipa Rosa
Vitor Vasconcelos
Lotfi Achour
Adnen Kacem
Ralph Urbatzka
The Marine Seagrass <i>Halophila stipulacea</i> as a Source of Bioactive Metabolites against Obesity and Biofouling
Marine Drugs
<i>halophila stipulacea</i>
extracts
bioactivity
cytotoxicity
anti-obesity
antifouling
title The Marine Seagrass <i>Halophila stipulacea</i> as a Source of Bioactive Metabolites against Obesity and Biofouling
title_full The Marine Seagrass <i>Halophila stipulacea</i> as a Source of Bioactive Metabolites against Obesity and Biofouling
title_fullStr The Marine Seagrass <i>Halophila stipulacea</i> as a Source of Bioactive Metabolites against Obesity and Biofouling
title_full_unstemmed The Marine Seagrass <i>Halophila stipulacea</i> as a Source of Bioactive Metabolites against Obesity and Biofouling
title_short The Marine Seagrass <i>Halophila stipulacea</i> as a Source of Bioactive Metabolites against Obesity and Biofouling
title_sort marine seagrass i halophila stipulacea i as a source of bioactive metabolites against obesity and biofouling
topic <i>halophila stipulacea</i>
extracts
bioactivity
cytotoxicity
anti-obesity
antifouling
url https://www.mdpi.com/1660-3397/18/2/88
work_keys_str_mv AT sawssenbelmabrouk themarineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT marianareis themarineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT marialigiasousa themarineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT tiagoribeiro themarineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT joanaralmeida themarineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT sandrapereira themarineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT jorgeantunes themarineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT filiparosa themarineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT vitorvasconcelos themarineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT lotfiachour themarineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT adnenkacem themarineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT ralphurbatzka themarineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT sawssenbelmabrouk marineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT marianareis marineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT marialigiasousa marineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT tiagoribeiro marineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT joanaralmeida marineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT sandrapereira marineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT jorgeantunes marineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT filiparosa marineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT vitorvasconcelos marineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT lotfiachour marineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT adnenkacem marineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling
AT ralphurbatzka marineseagrassihalophilastipulaceaiasasourceofbioactivemetabolitesagainstobesityandbiofouling