Cecal Infusion of Sodium Propionate Promotes Intestinal Development and Jejunal Barrier Function in Growing Pigs
Short-chain fatty acids (SCFAs) produced by microbial fermentation facilitate the differentiation and proliferation of intestinal epithelium. However, the role of individual SCFAs, such as propionate, on intestinal development is still unclear. In the present study, sixteen barrows fitted with a cec...
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2019-05-01
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author | Yanan Zhang Huizi Chen Weiyun Zhu Kaifan Yu |
author_facet | Yanan Zhang Huizi Chen Weiyun Zhu Kaifan Yu |
author_sort | Yanan Zhang |
collection | DOAJ |
description | Short-chain fatty acids (SCFAs) produced by microbial fermentation facilitate the differentiation and proliferation of intestinal epithelium. However, the role of individual SCFAs, such as propionate, on intestinal development is still unclear. In the present study, sixteen barrows fitted with a cecal fistula were randomly divided into two groups for cecal infusion of either saline (control group) or sodium propionate (propionate group). After 28 days, the length and the relative weight of intestinal segments were calculated, the intestinal morphology was assessed, and the expression of tight junction protein was measured using qPCR and Western blotting. Compared to the saline group, the length of the colon was significantly increased in the propionate group (<i>p</i> < 0.05). The jejunal villi length and villi/crypt ratio in the propionate group were significantly higher than in the saline group (<i>p</i> < 0.05). Furthermore, propionate infusion significantly upregulated the mRNA levels of <i>Claudin-4</i> and the expression of Claudin-1, Claudin-4, and Occludin protein in the jejunal mucosa (<i>p</i> < 0.05). Collectively, these findings revealed that the short-chain fatty acid propionate in the hindgut contributed to intestinal development, and selectively enhanced jejunal tight junction protein expression. |
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spelling | doaj.art-da916e24e084496d996b2ea1aa9c8d9e2022-12-22T00:08:31ZengMDPI AGAnimals2076-26152019-05-019628410.3390/ani9060284ani9060284Cecal Infusion of Sodium Propionate Promotes Intestinal Development and Jejunal Barrier Function in Growing PigsYanan Zhang0Huizi Chen1Weiyun Zhu2Kaifan Yu3Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaShort-chain fatty acids (SCFAs) produced by microbial fermentation facilitate the differentiation and proliferation of intestinal epithelium. However, the role of individual SCFAs, such as propionate, on intestinal development is still unclear. In the present study, sixteen barrows fitted with a cecal fistula were randomly divided into two groups for cecal infusion of either saline (control group) or sodium propionate (propionate group). After 28 days, the length and the relative weight of intestinal segments were calculated, the intestinal morphology was assessed, and the expression of tight junction protein was measured using qPCR and Western blotting. Compared to the saline group, the length of the colon was significantly increased in the propionate group (<i>p</i> < 0.05). The jejunal villi length and villi/crypt ratio in the propionate group were significantly higher than in the saline group (<i>p</i> < 0.05). Furthermore, propionate infusion significantly upregulated the mRNA levels of <i>Claudin-4</i> and the expression of Claudin-1, Claudin-4, and Occludin protein in the jejunal mucosa (<i>p</i> < 0.05). Collectively, these findings revealed that the short-chain fatty acid propionate in the hindgut contributed to intestinal development, and selectively enhanced jejunal tight junction protein expression.https://www.mdpi.com/2076-2615/9/6/284propionateintestinal developmenttight junctionpigs |
spellingShingle | Yanan Zhang Huizi Chen Weiyun Zhu Kaifan Yu Cecal Infusion of Sodium Propionate Promotes Intestinal Development and Jejunal Barrier Function in Growing Pigs Animals propionate intestinal development tight junction pigs |
title | Cecal Infusion of Sodium Propionate Promotes Intestinal Development and Jejunal Barrier Function in Growing Pigs |
title_full | Cecal Infusion of Sodium Propionate Promotes Intestinal Development and Jejunal Barrier Function in Growing Pigs |
title_fullStr | Cecal Infusion of Sodium Propionate Promotes Intestinal Development and Jejunal Barrier Function in Growing Pigs |
title_full_unstemmed | Cecal Infusion of Sodium Propionate Promotes Intestinal Development and Jejunal Barrier Function in Growing Pigs |
title_short | Cecal Infusion of Sodium Propionate Promotes Intestinal Development and Jejunal Barrier Function in Growing Pigs |
title_sort | cecal infusion of sodium propionate promotes intestinal development and jejunal barrier function in growing pigs |
topic | propionate intestinal development tight junction pigs |
url | https://www.mdpi.com/2076-2615/9/6/284 |
work_keys_str_mv | AT yananzhang cecalinfusionofsodiumpropionatepromotesintestinaldevelopmentandjejunalbarrierfunctioningrowingpigs AT huizichen cecalinfusionofsodiumpropionatepromotesintestinaldevelopmentandjejunalbarrierfunctioningrowingpigs AT weiyunzhu cecalinfusionofsodiumpropionatepromotesintestinaldevelopmentandjejunalbarrierfunctioningrowingpigs AT kaifanyu cecalinfusionofsodiumpropionatepromotesintestinaldevelopmentandjejunalbarrierfunctioningrowingpigs |