Biocontrol and Plant-Growth-Promoting Traits of <i>Talaromyces apiculatus</i> and <i>Clonostachys rosea</i> Consortium against Ganoderma Basal Stem Rot Disease of Oil Palm
Basal stem rot (BSR) disease caused by <i>Ganoderma boninense</i> basidiomycetous fungus is the most economically important disease in oil palms in South East Asia. Unfortunately, there is no single most effective control measure available. Tremendous efforts have been directed in incorp...
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MDPI AG
2020-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/8/1138 |
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| author | Yit Kheng Goh Nurul Fadhilah Marzuki Tuan Nur Fatihah Tuan Pa Teik-Khiang Goh Zeng Seng Kee You Keng Goh Mohd Termizi Yusof Vladimir Vujanovic Kah Joo Goh |
| author_facet | Yit Kheng Goh Nurul Fadhilah Marzuki Tuan Nur Fatihah Tuan Pa Teik-Khiang Goh Zeng Seng Kee You Keng Goh Mohd Termizi Yusof Vladimir Vujanovic Kah Joo Goh |
| author_sort | Yit Kheng Goh |
| collection | DOAJ |
| description | Basal stem rot (BSR) disease caused by <i>Ganoderma boninense</i> basidiomycetous fungus is the most economically important disease in oil palms in South East Asia. Unfortunately, there is no single most effective control measure available. Tremendous efforts have been directed in incorporation of environmentally friendly biocontrol approaches in minimizing BSR disease. This study investigated the performance of two potential biocontrol agents (BCAs), AAT0115 and AAB0114 strains recovered from oil palm on suppression of BSR in planta, and also assessed their plant-growth-promoting (PGP) performance. ITS rRNA-sequence phylogeny discriminated the two ascomycetous <i>Talaromyces apiculatus</i> (Ta) AT0115 and <i>Clonostachys rosea</i> (Cr) AAB0114 biocontrol species with PGP characteristics. In vitro studies have demonstrated both Ta and Cr are capable of reducing linear mycelial growth of <i>G. boninense.</i> Inoculation of individual Cr and Ta—as well as Cr+Ta consortium—induced a significant increment in leaf area and bole girth of oil-palm seedlings five months post-inoculation (MPI) under nursery conditions. At five months post-inoculation, shoot and root biomass, and nutrient contents (nitrogen, phosphorus, potassium, calcium, magnesium and boron) were significantly higher in Ta-inoculated seedlings compared to control treated with non-Ta-inoculated maize. Chlorophyll and carotenoids contents in rapidly growing oil-palm seedlings challenged with Cr, Ta or a combination of both were not negatively affected. Cr, Ta and Cr+Ta consortium treated seedlings had 4.9–60% BSR disease reduction compared to the untreated control. Co-inoculation of Cr and Ta resulted in increased BSR control efficiencies by 18–26% (compared with Cr only) and 48–55% (compared with Ta only). Collectively, Cr and Ta, either individually or in consortium showed potential as BSR biocontrol agents while also possess PGP traits in oil palm. |
| first_indexed | 2024-03-10T18:10:31Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
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| last_indexed | 2024-03-10T18:10:31Z |
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| spelling | doaj.art-da94b17cc83147db9a7a1cd1a74d58722023-11-20T08:12:32ZengMDPI AGMicroorganisms2076-26072020-07-0188113810.3390/microorganisms8081138Biocontrol and Plant-Growth-Promoting Traits of <i>Talaromyces apiculatus</i> and <i>Clonostachys rosea</i> Consortium against Ganoderma Basal Stem Rot Disease of Oil PalmYit Kheng Goh0Nurul Fadhilah Marzuki1Tuan Nur Fatihah Tuan Pa2Teik-Khiang Goh3Zeng Seng Kee4You Keng Goh5Mohd Termizi Yusof6Vladimir Vujanovic7Kah Joo Goh8Advanced Agriecological Research Sdn Bhd, Kota Damansara, Petaling Jaya 47810, MalaysiaAdvanced Agriecological Research Sdn Bhd, Kota Damansara, Petaling Jaya 47810, MalaysiaAdvanced Agriecological Research Sdn Bhd, Kota Damansara, Petaling Jaya 47810, MalaysiaAdvanced Agriecological Research Sdn Bhd, Kota Damansara, Petaling Jaya 47810, MalaysiaAdvanced Agriecological Research Sdn Bhd, Kota Damansara, Petaling Jaya 47810, MalaysiaAdvanced Agriecological Research Sdn Bhd, Kota Damansara, Petaling Jaya 47810, MalaysiaDepartment of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, Serdang 43400, MalaysiaDepartment of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK S7N 5A8, CanadaAdvanced Agriecological Research Sdn Bhd, Kota Damansara, Petaling Jaya 47810, MalaysiaBasal stem rot (BSR) disease caused by <i>Ganoderma boninense</i> basidiomycetous fungus is the most economically important disease in oil palms in South East Asia. Unfortunately, there is no single most effective control measure available. Tremendous efforts have been directed in incorporation of environmentally friendly biocontrol approaches in minimizing BSR disease. This study investigated the performance of two potential biocontrol agents (BCAs), AAT0115 and AAB0114 strains recovered from oil palm on suppression of BSR in planta, and also assessed their plant-growth-promoting (PGP) performance. ITS rRNA-sequence phylogeny discriminated the two ascomycetous <i>Talaromyces apiculatus</i> (Ta) AT0115 and <i>Clonostachys rosea</i> (Cr) AAB0114 biocontrol species with PGP characteristics. In vitro studies have demonstrated both Ta and Cr are capable of reducing linear mycelial growth of <i>G. boninense.</i> Inoculation of individual Cr and Ta—as well as Cr+Ta consortium—induced a significant increment in leaf area and bole girth of oil-palm seedlings five months post-inoculation (MPI) under nursery conditions. At five months post-inoculation, shoot and root biomass, and nutrient contents (nitrogen, phosphorus, potassium, calcium, magnesium and boron) were significantly higher in Ta-inoculated seedlings compared to control treated with non-Ta-inoculated maize. Chlorophyll and carotenoids contents in rapidly growing oil-palm seedlings challenged with Cr, Ta or a combination of both were not negatively affected. Cr, Ta and Cr+Ta consortium treated seedlings had 4.9–60% BSR disease reduction compared to the untreated control. Co-inoculation of Cr and Ta resulted in increased BSR control efficiencies by 18–26% (compared with Cr only) and 48–55% (compared with Ta only). Collectively, Cr and Ta, either individually or in consortium showed potential as BSR biocontrol agents while also possess PGP traits in oil palm.https://www.mdpi.com/2076-2607/8/8/1138antagonistic fungusascomycetebiologic controlmycoparasite<i>Ganoderma boninense</i>plant growth |
| spellingShingle | Yit Kheng Goh Nurul Fadhilah Marzuki Tuan Nur Fatihah Tuan Pa Teik-Khiang Goh Zeng Seng Kee You Keng Goh Mohd Termizi Yusof Vladimir Vujanovic Kah Joo Goh Biocontrol and Plant-Growth-Promoting Traits of <i>Talaromyces apiculatus</i> and <i>Clonostachys rosea</i> Consortium against Ganoderma Basal Stem Rot Disease of Oil Palm Microorganisms antagonistic fungus ascomycete biologic control mycoparasite <i>Ganoderma boninense</i> plant growth |
| title | Biocontrol and Plant-Growth-Promoting Traits of <i>Talaromyces apiculatus</i> and <i>Clonostachys rosea</i> Consortium against Ganoderma Basal Stem Rot Disease of Oil Palm |
| title_full | Biocontrol and Plant-Growth-Promoting Traits of <i>Talaromyces apiculatus</i> and <i>Clonostachys rosea</i> Consortium against Ganoderma Basal Stem Rot Disease of Oil Palm |
| title_fullStr | Biocontrol and Plant-Growth-Promoting Traits of <i>Talaromyces apiculatus</i> and <i>Clonostachys rosea</i> Consortium against Ganoderma Basal Stem Rot Disease of Oil Palm |
| title_full_unstemmed | Biocontrol and Plant-Growth-Promoting Traits of <i>Talaromyces apiculatus</i> and <i>Clonostachys rosea</i> Consortium against Ganoderma Basal Stem Rot Disease of Oil Palm |
| title_short | Biocontrol and Plant-Growth-Promoting Traits of <i>Talaromyces apiculatus</i> and <i>Clonostachys rosea</i> Consortium against Ganoderma Basal Stem Rot Disease of Oil Palm |
| title_sort | biocontrol and plant growth promoting traits of i talaromyces apiculatus i and i clonostachys rosea i consortium against ganoderma basal stem rot disease of oil palm |
| topic | antagonistic fungus ascomycete biologic control mycoparasite <i>Ganoderma boninense</i> plant growth |
| url | https://www.mdpi.com/2076-2607/8/8/1138 |
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