The Role of Noncoding RNA Antisense Transcript of the B-Cell Translocation Gene 3 Regulation of BTG3 in Pancreatic Ductal Adenocarcinoma Tumor Progression
ABSTRACT: Background: Antisense transcript of the B-cell translocation gene 3 (ASBEL) is a highly conserved antisense non-coding RNA (ncRNA) and participates in a variety of biological processes. However, the ASBEL expression status in pancreatic ductal adenocarcinoma (PDAC) and its correlation wit...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Current Therapeutic Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0011393X23000103 |
| _version_ | 1797799179319246848 |
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| author | Jing Chen, M.D. Ming-Yuan Zhu, M.D. Yan-Hua Huang, M.D. Yi-Ting Ling, M.D. Tian-Yuan Gu, M.D. Quan Zhou, M.D. Ming-Jian Fei, M.D. Zhong-Cheng Zhou, M.D. |
| author_facet | Jing Chen, M.D. Ming-Yuan Zhu, M.D. Yan-Hua Huang, M.D. Yi-Ting Ling, M.D. Tian-Yuan Gu, M.D. Quan Zhou, M.D. Ming-Jian Fei, M.D. Zhong-Cheng Zhou, M.D. |
| author_sort | Jing Chen, M.D. |
| collection | DOAJ |
| description | ABSTRACT: Background: Antisense transcript of the B-cell translocation gene 3 (ASBEL) is a highly conserved antisense non-coding RNA (ncRNA) and participates in a variety of biological processes. However, the ASBEL expression status in pancreatic ductal adenocarcinoma (PDAC) and its correlation with BTG3 expression and tumor cell progression were not completely clear. Objective: We conducted cell experiments and animal experiments to confirm that ASBEL plays a crucial role in the tumorigenesis of PDAC by targeting BTG3. Methods: ASBEL regulation in PDAC tumorigenesis was evaluated using Western blotting, quantitative polymerase chain reaction, Cell Counting Kit-8 assay, flow cytometry, and cell transfection. We also evaluated the expression of ASBEL and BTG3 in tumor tissues and cells using Western blotting and quantitative real-time polymerase chain reaction. Finally, we explored the role of ASBEL in tumor development by silencing or overexpressing ASBEL gene in AsPC-1 or CFPAC-1 cells, respectively, and evaluated the antitumor activity in vivo using an ASBEL antagonist. Results: Our study revealed the expression of ASBEL in all pancreatic cell lines. The expression level of ASBEL in tumor tissues was found to be higher than that of paracarcinomatous tissues. ASBEL suppresses expression of BTG3, enhances proliferation and suppresses apoptosis, and promotes migration and invasion in pancreatic cancer cell. Antagonist regulates the expression of ASBEL in AsPC-1, and suppresses tumor growth in xenograft mouse model. Conclusions: Our results indicate that ASBEL may play a tumor-promoting factor in PDAC by targeting BTG3 and could be as an important biomarker for PDAC treatment. (Curr Ther Res Clin Exp. 2023; 84:XXX–XXX). |
| first_indexed | 2024-03-13T04:15:03Z |
| format | Article |
| id | doaj.art-da97692f5541458d85dc53d91356ea0f |
| institution | Directory Open Access Journal |
| issn | 0011-393X |
| language | English |
| last_indexed | 2024-03-13T04:15:03Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Therapeutic Research |
| spelling | doaj.art-da97692f5541458d85dc53d91356ea0f2023-06-21T06:50:45ZengElsevierCurrent Therapeutic Research0011-393X2023-01-0198100700The Role of Noncoding RNA Antisense Transcript of the B-Cell Translocation Gene 3 Regulation of BTG3 in Pancreatic Ductal Adenocarcinoma Tumor ProgressionJing Chen, M.D.0Ming-Yuan Zhu, M.D.1Yan-Hua Huang, M.D.2Yi-Ting Ling, M.D.3Tian-Yuan Gu, M.D.4Quan Zhou, M.D.5Ming-Jian Fei, M.D.6Zhong-Cheng Zhou, M.D.7Department of General Surgery, The Second Affiliated Hospital of Jiaxing University, Zhejiang Province, ChinaDepartment of General Surgery, The Second Affiliated Hospital of Jiaxing University, Zhejiang Province, ChinaDepartment of General Surgery, The Second Affiliated Hospital of Jiaxing University, Zhejiang Province, ChinaDepartment of General Surgery, The Second Affiliated Hospital of Jiaxing University, Zhejiang Province, ChinaDepartment of General Surgery, The Second Affiliated Hospital of Jiaxing University, Zhejiang Province, ChinaDepartment of Pathology, The Second Affiliated Hospital of Jiaxing University, Zhejiang Province, ChinaDepartment of Pathology, The Second Affiliated Hospital of Jiaxing University, Zhejiang Province, ChinaDepartment of General Surgery, The Second Affiliated Hospital of Jiaxing University, Zhejiang Province, China; Address correspondence to: Zhong-Cheng Zhou, M.D., 1517, Huancheng North Road, Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Jiaxing University, Jiaxing 314000, Zhejiang Province, China.ABSTRACT: Background: Antisense transcript of the B-cell translocation gene 3 (ASBEL) is a highly conserved antisense non-coding RNA (ncRNA) and participates in a variety of biological processes. However, the ASBEL expression status in pancreatic ductal adenocarcinoma (PDAC) and its correlation with BTG3 expression and tumor cell progression were not completely clear. Objective: We conducted cell experiments and animal experiments to confirm that ASBEL plays a crucial role in the tumorigenesis of PDAC by targeting BTG3. Methods: ASBEL regulation in PDAC tumorigenesis was evaluated using Western blotting, quantitative polymerase chain reaction, Cell Counting Kit-8 assay, flow cytometry, and cell transfection. We also evaluated the expression of ASBEL and BTG3 in tumor tissues and cells using Western blotting and quantitative real-time polymerase chain reaction. Finally, we explored the role of ASBEL in tumor development by silencing or overexpressing ASBEL gene in AsPC-1 or CFPAC-1 cells, respectively, and evaluated the antitumor activity in vivo using an ASBEL antagonist. Results: Our study revealed the expression of ASBEL in all pancreatic cell lines. The expression level of ASBEL in tumor tissues was found to be higher than that of paracarcinomatous tissues. ASBEL suppresses expression of BTG3, enhances proliferation and suppresses apoptosis, and promotes migration and invasion in pancreatic cancer cell. Antagonist regulates the expression of ASBEL in AsPC-1, and suppresses tumor growth in xenograft mouse model. Conclusions: Our results indicate that ASBEL may play a tumor-promoting factor in PDAC by targeting BTG3 and could be as an important biomarker for PDAC treatment. (Curr Ther Res Clin Exp. 2023; 84:XXX–XXX).http://www.sciencedirect.com/science/article/pii/S0011393X23000103ASBELAntagonistLong chain noncoding RNAPancreatic ductal adenocarcinomaRNA interference |
| spellingShingle | Jing Chen, M.D. Ming-Yuan Zhu, M.D. Yan-Hua Huang, M.D. Yi-Ting Ling, M.D. Tian-Yuan Gu, M.D. Quan Zhou, M.D. Ming-Jian Fei, M.D. Zhong-Cheng Zhou, M.D. The Role of Noncoding RNA Antisense Transcript of the B-Cell Translocation Gene 3 Regulation of BTG3 in Pancreatic Ductal Adenocarcinoma Tumor Progression Current Therapeutic Research ASBEL Antagonist Long chain noncoding RNA Pancreatic ductal adenocarcinoma RNA interference |
| title | The Role of Noncoding RNA Antisense Transcript of the B-Cell Translocation Gene 3 Regulation of BTG3 in Pancreatic Ductal Adenocarcinoma Tumor Progression |
| title_full | The Role of Noncoding RNA Antisense Transcript of the B-Cell Translocation Gene 3 Regulation of BTG3 in Pancreatic Ductal Adenocarcinoma Tumor Progression |
| title_fullStr | The Role of Noncoding RNA Antisense Transcript of the B-Cell Translocation Gene 3 Regulation of BTG3 in Pancreatic Ductal Adenocarcinoma Tumor Progression |
| title_full_unstemmed | The Role of Noncoding RNA Antisense Transcript of the B-Cell Translocation Gene 3 Regulation of BTG3 in Pancreatic Ductal Adenocarcinoma Tumor Progression |
| title_short | The Role of Noncoding RNA Antisense Transcript of the B-Cell Translocation Gene 3 Regulation of BTG3 in Pancreatic Ductal Adenocarcinoma Tumor Progression |
| title_sort | role of noncoding rna antisense transcript of the b cell translocation gene 3 regulation of btg3 in pancreatic ductal adenocarcinoma tumor progression |
| topic | ASBEL Antagonist Long chain noncoding RNA Pancreatic ductal adenocarcinoma RNA interference |
| url | http://www.sciencedirect.com/science/article/pii/S0011393X23000103 |
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